Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation

Chien-Yu Chiu,1,2 Tze-Wen Chung,3,4 Si-Yi Chen,2 Yunn-Hwa Ma2,51Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan, Taoyuan City 33302, Taiwan, ROC; 2Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, G...

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Autores principales:	Chiu CY, Chung TW, Chen SY, Ma YH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	polyethylene glycol magnetic nanoparticles hemodynamics microcirculation magnetic targeting Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/989b3c7bc5cd4020a50cb54982ac9bb9
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spelling	oai:doaj.org-article:989b3c7bc5cd4020a50cb54982ac9bb92021-12-02T04:14:58ZEffects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation1178-2013https://doaj.org/article/989b3c7bc5cd4020a50cb54982ac9bb92019-07-01T00:00:00Zhttps://www.dovepress.com/effects-of-pegylation-on-capture-of-dextran-coated-magnetic-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chien-Yu Chiu,1,2 Tze-Wen Chung,3,4 Si-Yi Chen,2 Yunn-Hwa Ma2,51Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan, Taoyuan City 33302, Taiwan, ROC; 2Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Guishan, Taoyuan City 33302, Taiwan, ROC; 3Department of Biomedical Engineering, National Yang-Ming University, Beitou, Taipei City 11221, Taiwan, ROC; 4Center for Advanced Pharmaceutical Research and Drug Delivery, National Yang-Ming University, Beitou, Taipei City 11221, Taiwan, ROC; 5Department of Neurology, Chang Gung Memorial Hospital, Guishan, Taoyuan City 33305, Taiwan, ROCBackground: Magnetic nanoparticles (MNPs) can be localized against hemodynamic forces in blood vessels with the application of an external magnetic field. In addition, PEGylation of nanoparticles may increase the half-life of nanocomposites in circulation. In this work, we examined the effect of PEGylation on the magnetic capture of MNPs in vivo.Methods: Laser speckle contrast imaging and capillaroscopy were used to assess the magnetic capture of dextran-coated MNPs and red blood cell (RBC) flow in cremaster microvessels of anesthetized rats. Magnetic capture of MNPs in serum flow was visualized with an in vitro circulating system. The effect of PEGylation on MNP-endothelial cell interaction was studied in cultured cells using an iron assay.Results: In microcirculation through cremaster muscle, magnet-induced retention of 250 nm MNPs was associated with a variable reduction in RBC flow, suggesting a dynamic coupling of hemodynamic and magnetic forces. After magnet removal, faster restoration of flow was observed in PEG(+) than PEG(–) group, which may be attributed to a reduced interaction with vascular endothelium. However, PEGylation appears to be required for magnetic capture of 50 nm MNPs in microvessels, which was associated with increased hydrodynamic diameter to 130±6 nm in serum, but independent of the &sigmaf;-potential.Conclusion: These results suggest that PEGylation may enhance magnetic capture of smaller MNPs and dispersion of larger MNPs after magnet removal, which may potentially affect the targeting, pharmacokinetics and therapeutic efficacy.Keywords: polyethylene glycol, magnetic nanoparticles, hemodynamics, microcirculation, magnetic targetingChiu CYChung TWChen SYMa YHDove Medical Pressarticlepolyethylene glycolmagnetic nanoparticleshemodynamicsmicrocirculationmagnetic targetingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4767-4780 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyethylene glycol magnetic nanoparticles hemodynamics microcirculation magnetic targeting Medicine (General) R5-920
spellingShingle	polyethylene glycol magnetic nanoparticles hemodynamics microcirculation magnetic targeting Medicine (General) R5-920 Chiu CY Chung TW Chen SY Ma YH Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
description	Chien-Yu Chiu,1,2 Tze-Wen Chung,3,4 Si-Yi Chen,2 Yunn-Hwa Ma2,51Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan, Taoyuan City 33302, Taiwan, ROC; 2Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Guishan, Taoyuan City 33302, Taiwan, ROC; 3Department of Biomedical Engineering, National Yang-Ming University, Beitou, Taipei City 11221, Taiwan, ROC; 4Center for Advanced Pharmaceutical Research and Drug Delivery, National Yang-Ming University, Beitou, Taipei City 11221, Taiwan, ROC; 5Department of Neurology, Chang Gung Memorial Hospital, Guishan, Taoyuan City 33305, Taiwan, ROCBackground: Magnetic nanoparticles (MNPs) can be localized against hemodynamic forces in blood vessels with the application of an external magnetic field. In addition, PEGylation of nanoparticles may increase the half-life of nanocomposites in circulation. In this work, we examined the effect of PEGylation on the magnetic capture of MNPs in vivo.Methods: Laser speckle contrast imaging and capillaroscopy were used to assess the magnetic capture of dextran-coated MNPs and red blood cell (RBC) flow in cremaster microvessels of anesthetized rats. Magnetic capture of MNPs in serum flow was visualized with an in vitro circulating system. The effect of PEGylation on MNP-endothelial cell interaction was studied in cultured cells using an iron assay.Results: In microcirculation through cremaster muscle, magnet-induced retention of 250 nm MNPs was associated with a variable reduction in RBC flow, suggesting a dynamic coupling of hemodynamic and magnetic forces. After magnet removal, faster restoration of flow was observed in PEG(+) than PEG(–) group, which may be attributed to a reduced interaction with vascular endothelium. However, PEGylation appears to be required for magnetic capture of 50 nm MNPs in microvessels, which was associated with increased hydrodynamic diameter to 130±6 nm in serum, but independent of the &sigmaf;-potential.Conclusion: These results suggest that PEGylation may enhance magnetic capture of smaller MNPs and dispersion of larger MNPs after magnet removal, which may potentially affect the targeting, pharmacokinetics and therapeutic efficacy.Keywords: polyethylene glycol, magnetic nanoparticles, hemodynamics, microcirculation, magnetic targeting
format	article
author	Chiu CY Chung TW Chen SY Ma YH
author_facet	Chiu CY Chung TW Chen SY Ma YH
author_sort	Chiu CY
title	Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
title_short	Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
title_full	Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
title_fullStr	Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
title_full_unstemmed	Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation
title_sort	effects of pegylation on capture of dextran-coated magnetic nanoparticles in microcirculation
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/989b3c7bc5cd4020a50cb54982ac9bb9
work_keys_str_mv	AT chiucy effectsofpegylationoncaptureofdextrancoatedmagneticnanoparticlesinmicrocirculation AT chungtw effectsofpegylationoncaptureofdextrancoatedmagneticnanoparticlesinmicrocirculation AT chensy effectsofpegylationoncaptureofdextrancoatedmagneticnanoparticlesinmicrocirculation AT mayh effectsofpegylationoncaptureofdextrancoatedmagneticnanoparticlesinmicrocirculation
_version_	1718401386874404864

Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation

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