Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells

Abstract Mechanisms mediating adult enteric neurogenesis are largely unknown. Using inflammation-associated neurogenesis models and a transgenic approach, we aimed to understand the cell-source for new neurons in infectious and inflammatory colitis. Dextran sodium sulfate (DSS) and Citrobacter roden...

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Autores principales:	Jaime Belkind-Gerson, Hannah K. Graham, Justin Reynolds, Ryo Hotta, Nandor Nagy, Lily Cheng, Michal Kamionek, Hai Ning Shi, Carol M. Aherne, Allan M. Goldstein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98a0aa1a66ae46da8336099aaa713215
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spelling	oai:doaj.org-article:98a0aa1a66ae46da8336099aaa7132152021-12-02T15:06:15ZColitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells10.1038/s41598-017-02890-y2045-2322https://doaj.org/article/98a0aa1a66ae46da8336099aaa7132152017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02890-yhttps://doaj.org/toc/2045-2322Abstract Mechanisms mediating adult enteric neurogenesis are largely unknown. Using inflammation-associated neurogenesis models and a transgenic approach, we aimed to understand the cell-source for new neurons in infectious and inflammatory colitis. Dextran sodium sulfate (DSS) and Citrobacter rodentium colitis (CC) was induced in adult mice and colonic neurons were quantified. Sox2GFP and PLP1GFP mice confirmed the cell-type specificity of these markers. Sox2CreER:YFP and PLP1creER:tdT mice were used to determine the fate of these cells after colitis. Sox2 expression was investigated in colonic neurons of human patients with Clostridium difficile or ulcerative colitis. Both DSS and CC led to increased colonic neurons. Following colitis in adult Sox2CreER:YFP mice, YFP initially expressed predominantly by glia becomes expressed by neurons following colitis, without observable DNA replication. Similarly in PLP1CreER:tdT mice, PLP1 cells that co-express S100b but not RET also give rise to neurons following colitis. In human colitis, Sox2-expressing neurons increase from 1–2% to an average 14% in colitis. The new neurons predominantly express calretinin, thus appear to be excitatory. These results suggest that colitis promotes rapid enteric neurogenesis in adult mice and humans through differentiation of Sox2- and PLP1-expressing cells, which represent enteric glia and/or neural progenitors. Further defining neurogenesis will improve understanding and treatment of injury-associated intestinal motility/sensory disorders.Jaime Belkind-GersonHannah K. GrahamJustin ReynoldsRyo HottaNandor NagyLily ChengMichal KamionekHai Ning ShiCarol M. AherneAllan M. GoldsteinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaime Belkind-Gerson Hannah K. Graham Justin Reynolds Ryo Hotta Nandor Nagy Lily Cheng Michal Kamionek Hai Ning Shi Carol M. Aherne Allan M. Goldstein Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
description	Abstract Mechanisms mediating adult enteric neurogenesis are largely unknown. Using inflammation-associated neurogenesis models and a transgenic approach, we aimed to understand the cell-source for new neurons in infectious and inflammatory colitis. Dextran sodium sulfate (DSS) and Citrobacter rodentium colitis (CC) was induced in adult mice and colonic neurons were quantified. Sox2GFP and PLP1GFP mice confirmed the cell-type specificity of these markers. Sox2CreER:YFP and PLP1creER:tdT mice were used to determine the fate of these cells after colitis. Sox2 expression was investigated in colonic neurons of human patients with Clostridium difficile or ulcerative colitis. Both DSS and CC led to increased colonic neurons. Following colitis in adult Sox2CreER:YFP mice, YFP initially expressed predominantly by glia becomes expressed by neurons following colitis, without observable DNA replication. Similarly in PLP1CreER:tdT mice, PLP1 cells that co-express S100b but not RET also give rise to neurons following colitis. In human colitis, Sox2-expressing neurons increase from 1–2% to an average 14% in colitis. The new neurons predominantly express calretinin, thus appear to be excitatory. These results suggest that colitis promotes rapid enteric neurogenesis in adult mice and humans through differentiation of Sox2- and PLP1-expressing cells, which represent enteric glia and/or neural progenitors. Further defining neurogenesis will improve understanding and treatment of injury-associated intestinal motility/sensory disorders.
format	article
author	Jaime Belkind-Gerson Hannah K. Graham Justin Reynolds Ryo Hotta Nandor Nagy Lily Cheng Michal Kamionek Hai Ning Shi Carol M. Aherne Allan M. Goldstein
author_facet	Jaime Belkind-Gerson Hannah K. Graham Justin Reynolds Ryo Hotta Nandor Nagy Lily Cheng Michal Kamionek Hai Ning Shi Carol M. Aherne Allan M. Goldstein
author_sort	Jaime Belkind-Gerson
title	Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
title_short	Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
title_full	Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
title_fullStr	Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
title_full_unstemmed	Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
title_sort	colitis promotes neuronal differentiation of sox2+ and plp1+ enteric cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/98a0aa1a66ae46da8336099aaa713215
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_version_	1718388530474909696

Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells

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