Pancreatic islet macroencapsulation using microwell porous membranes

Abstract Allogeneic islet transplantation into the liver in combination with immune suppressive drug therapy is widely regarded as a potential cure for type 1 diabetes. However, the intrahepatic system is suboptimal as the concentration of drugs and nutrients there is higher compared to pancreas, wh...

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Autores principales:	Katarzyna Skrzypek, Milou Groot Nibbelink, Jéré van Lente, Mijke Buitinga, Marten A. Engelse, Eelco J. P. de Koning, Marcel Karperien, Aart van Apeldoorn, Dimitrios Stamatialis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98a2240eb1094834a9e554ca7f3d85d2
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spelling	oai:doaj.org-article:98a2240eb1094834a9e554ca7f3d85d22021-12-02T12:32:55ZPancreatic islet macroencapsulation using microwell porous membranes10.1038/s41598-017-09647-72045-2322https://doaj.org/article/98a2240eb1094834a9e554ca7f3d85d22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09647-7https://doaj.org/toc/2045-2322Abstract Allogeneic islet transplantation into the liver in combination with immune suppressive drug therapy is widely regarded as a potential cure for type 1 diabetes. However, the intrahepatic system is suboptimal as the concentration of drugs and nutrients there is higher compared to pancreas, which negatively affects islet function. Islet encapsulation within semipermeable membranes is a promising strategy that allows for the islet transplantation outside the suboptimal liver portal system and provides environment, where islets can perform their endocrine function. In this study, we develop a macroencapsulation device based on thin microwell membranes. The islets are seeded in separate microwells to avoid aggregation, whereas the membrane porosity is tailored to achieve sufficient transport of nutrients, glucose and insulin. The non-degradable, microwell membranes are composed of poly (ether sulfone)/polyvinylpyrrolidone and manufactured via phase separation micro molding. Our results show that the device prevents aggregation and preserves the islet’s native morphology. Moreover, the encapsulated islets maintain their glucose responsiveness and function after 7 days of culture (stimulation index above 2 for high glucose stimulation), demonstrating the potential of this novel device for islet transplantation.Katarzyna SkrzypekMilou Groot NibbelinkJéré van LenteMijke BuitingaMarten A. EngelseEelco J. P. de KoningMarcel KarperienAart van ApeldoornDimitrios StamatialisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Skrzypek Milou Groot Nibbelink Jéré van Lente Mijke Buitinga Marten A. Engelse Eelco J. P. de Koning Marcel Karperien Aart van Apeldoorn Dimitrios Stamatialis Pancreatic islet macroencapsulation using microwell porous membranes
description	Abstract Allogeneic islet transplantation into the liver in combination with immune suppressive drug therapy is widely regarded as a potential cure for type 1 diabetes. However, the intrahepatic system is suboptimal as the concentration of drugs and nutrients there is higher compared to pancreas, which negatively affects islet function. Islet encapsulation within semipermeable membranes is a promising strategy that allows for the islet transplantation outside the suboptimal liver portal system and provides environment, where islets can perform their endocrine function. In this study, we develop a macroencapsulation device based on thin microwell membranes. The islets are seeded in separate microwells to avoid aggregation, whereas the membrane porosity is tailored to achieve sufficient transport of nutrients, glucose and insulin. The non-degradable, microwell membranes are composed of poly (ether sulfone)/polyvinylpyrrolidone and manufactured via phase separation micro molding. Our results show that the device prevents aggregation and preserves the islet’s native morphology. Moreover, the encapsulated islets maintain their glucose responsiveness and function after 7 days of culture (stimulation index above 2 for high glucose stimulation), demonstrating the potential of this novel device for islet transplantation.
format	article
author	Katarzyna Skrzypek Milou Groot Nibbelink Jéré van Lente Mijke Buitinga Marten A. Engelse Eelco J. P. de Koning Marcel Karperien Aart van Apeldoorn Dimitrios Stamatialis
author_facet	Katarzyna Skrzypek Milou Groot Nibbelink Jéré van Lente Mijke Buitinga Marten A. Engelse Eelco J. P. de Koning Marcel Karperien Aart van Apeldoorn Dimitrios Stamatialis
author_sort	Katarzyna Skrzypek
title	Pancreatic islet macroencapsulation using microwell porous membranes
title_short	Pancreatic islet macroencapsulation using microwell porous membranes
title_full	Pancreatic islet macroencapsulation using microwell porous membranes
title_fullStr	Pancreatic islet macroencapsulation using microwell porous membranes
title_full_unstemmed	Pancreatic islet macroencapsulation using microwell porous membranes
title_sort	pancreatic islet macroencapsulation using microwell porous membranes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/98a2240eb1094834a9e554ca7f3d85d2
work_keys_str_mv	AT katarzynaskrzypek pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT milougrootnibbelink pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT jerevanlente pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT mijkebuitinga pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT martenaengelse pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT eelcojpdekoning pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT marcelkarperien pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT aartvanapeldoorn pancreaticisletmacroencapsulationusingmicrowellporousmembranes AT dimitriosstamatialis pancreaticisletmacroencapsulationusingmicrowellporousmembranes
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Pancreatic islet macroencapsulation using microwell porous membranes

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