Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome

Abstract Sophora flavescens are widely used for their pharmacological effects. As its main pharmacological components, alkaloids and flavonoids are distributed in the root tissues wherein molecular mechanisms remain elusive. In this study, metabolite profiles are analyzed using metabolomes to obtain...

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Autores principales:	Guangfei Wei, Yongzhong Chen, Xiaotong Guo, Jianhe Wei, Linlin Dong, Shilin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98a3c6dc0f33472ca12782cb43dfe19c
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spelling	oai:doaj.org-article:98a3c6dc0f33472ca12782cb43dfe19c2021-12-02T13:26:58ZBiosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome10.1038/s41598-021-86970-02045-2322https://doaj.org/article/98a3c6dc0f33472ca12782cb43dfe19c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86970-0https://doaj.org/toc/2045-2322Abstract Sophora flavescens are widely used for their pharmacological effects. As its main pharmacological components, alkaloids and flavonoids are distributed in the root tissues wherein molecular mechanisms remain elusive. In this study, metabolite profiles are analyzed using metabolomes to obtain biomarkers detected in different root tissues. These biomarkers include alkaloids, phenylpropanoids, and flavonoids. The high-performance liquid chromatography analysis results indicate the differences in principal component contents. Oxymatrine, sophoridine, and matrine contents are the highest in the phloem, whereas trifolirhizin, maackiain, and kushenol I contents are the highest in the xylem. The transcript expression profiles also show tissue specificity in the roots. A total of 52 and 39 transcripts involved in alkaloid and flavonoid syntheses are found, respectively. Among them, the expression levels of LYSA1, LYSA2, AO2, AO6, PMT1, PMT17, PMT34, and PMT35 transcripts are highly and positively correlated with alkaloids contents. The expression levels of 4CL1, 4CL3, 4CL12, CHI5, CHI7, and CHI9 transcripts are markedly and positively correlated with flavonoids contents. Moreover, the quantitative profiles of alkaloids and flavonoids are provided, and the pivotal genes regulating their distribution in S. flavescens are determined. These results contribute to the existing data for the genetic improvement and target breeding of S. flavescens.Guangfei WeiYongzhong ChenXiaotong GuoJianhe WeiLinlin DongShilin ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guangfei Wei Yongzhong Chen Xiaotong Guo Jianhe Wei Linlin Dong Shilin Chen Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
description	Abstract Sophora flavescens are widely used for their pharmacological effects. As its main pharmacological components, alkaloids and flavonoids are distributed in the root tissues wherein molecular mechanisms remain elusive. In this study, metabolite profiles are analyzed using metabolomes to obtain biomarkers detected in different root tissues. These biomarkers include alkaloids, phenylpropanoids, and flavonoids. The high-performance liquid chromatography analysis results indicate the differences in principal component contents. Oxymatrine, sophoridine, and matrine contents are the highest in the phloem, whereas trifolirhizin, maackiain, and kushenol I contents are the highest in the xylem. The transcript expression profiles also show tissue specificity in the roots. A total of 52 and 39 transcripts involved in alkaloid and flavonoid syntheses are found, respectively. Among them, the expression levels of LYSA1, LYSA2, AO2, AO6, PMT1, PMT17, PMT34, and PMT35 transcripts are highly and positively correlated with alkaloids contents. The expression levels of 4CL1, 4CL3, 4CL12, CHI5, CHI7, and CHI9 transcripts are markedly and positively correlated with flavonoids contents. Moreover, the quantitative profiles of alkaloids and flavonoids are provided, and the pivotal genes regulating their distribution in S. flavescens are determined. These results contribute to the existing data for the genetic improvement and target breeding of S. flavescens.
format	article
author	Guangfei Wei Yongzhong Chen Xiaotong Guo Jianhe Wei Linlin Dong Shilin Chen
author_facet	Guangfei Wei Yongzhong Chen Xiaotong Guo Jianhe Wei Linlin Dong Shilin Chen
author_sort	Guangfei Wei
title	Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
title_short	Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
title_full	Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
title_fullStr	Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
title_full_unstemmed	Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome
title_sort	biosyntheses characterization of alkaloids and flavonoids in sophora flavescens by combining metabolome and transcriptome
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/98a3c6dc0f33472ca12782cb43dfe19c
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Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome

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