Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation

ABSTRACT Substantial concentrations of penicillin V potassium (PVK) have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, bacterial strains capabl...

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Autores principales:	Peng Wang, Chen Shen, Kaili Xu, Qinqin Cong, Zhe Dong, Luwei Li, Jinfeng Guo, Jialin Lu, Shouxin Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	antibiotic bacterial residue penicillin V potassium biodegradation Ochrobactrum tritici biotransformation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/98aab3eaf39e42e29a61509744f0a038
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spelling	oai:doaj.org-article:98aab3eaf39e42e29a61509744f0a0382021-11-15T15:29:16ZIsolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation10.1128/mSphere.00058-202379-5042https://doaj.org/article/98aab3eaf39e42e29a61509744f0a0382020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00058-20https://doaj.org/toc/2379-5042ABSTRACT Substantial concentrations of penicillin V potassium (PVK) have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, bacterial strains capable of degrading PVK were isolated from sludge and characterized. Strain X-2 was selected for biodegradation of PVK. Based on morphological observations and 16S rRNA gene sequencing, strain X-2 was identified as an Ochrobactrum tritici strain. To enhance the PVK degradation ability of PVK, a whole-cell biodegradation process of Ochrobactrum tritici X-2 was established and optimized. In the whole-cell biodegradation process, the optimal temperature and pH were 30°C and 7.0, respectively. Under the optimized conditions, the degradation rate using 0.5 mg/ml PVK reached 100% within 3 h. During biodegradation, two major metabolites were detected: penicilloic acid and phenolic acid. The present study provides a novel method for the biodegradation of PVK using Ochrobactrum tritici strains, which represent promising candidates for the industrial biodegradation of PVK. IMPORTANCE Substantial concentrations of penicillin V potassium (PVK) have been found in the environment, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, antibiotic-degrading bacterial strains for PVK were isolated from sludge and characterized. Ochrobactrum tritici was selected for the biodegradation of PVK with high efficiency. To enhance its PVK degradation ability, a whole-cell biodegradation process was established and optimized using Ochrobactrum tritici. The degradation rate with 0.5 mg/ml PVK reached 100% within 3 h. The potential biodegradation pathway was also investigated. To the best of our knowledge, the present study provides new insights into the biodegradation of PVK using an Ochrobactrum tritici strain, a promising candidate strain for the industrial biodegradation of β-lactam antibiotics.Peng WangChen ShenKaili XuQinqin CongZhe DongLuwei LiJinfeng GuoJialin LuShouxin LiuAmerican Society for Microbiologyarticleantibioticbacterial residuepenicillin V potassiumbiodegradationOchrobactrum triticibiotransformationMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic bacterial residue penicillin V potassium biodegradation Ochrobactrum tritici biotransformation Microbiology QR1-502
spellingShingle	antibiotic bacterial residue penicillin V potassium biodegradation Ochrobactrum tritici biotransformation Microbiology QR1-502 Peng Wang Chen Shen Kaili Xu Qinqin Cong Zhe Dong Luwei Li Jinfeng Guo Jialin Lu Shouxin Liu Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
description	ABSTRACT Substantial concentrations of penicillin V potassium (PVK) have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, bacterial strains capable of degrading PVK were isolated from sludge and characterized. Strain X-2 was selected for biodegradation of PVK. Based on morphological observations and 16S rRNA gene sequencing, strain X-2 was identified as an Ochrobactrum tritici strain. To enhance the PVK degradation ability of PVK, a whole-cell biodegradation process of Ochrobactrum tritici X-2 was established and optimized. In the whole-cell biodegradation process, the optimal temperature and pH were 30°C and 7.0, respectively. Under the optimized conditions, the degradation rate using 0.5 mg/ml PVK reached 100% within 3 h. During biodegradation, two major metabolites were detected: penicilloic acid and phenolic acid. The present study provides a novel method for the biodegradation of PVK using Ochrobactrum tritici strains, which represent promising candidates for the industrial biodegradation of PVK. IMPORTANCE Substantial concentrations of penicillin V potassium (PVK) have been found in the environment, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, antibiotic-degrading bacterial strains for PVK were isolated from sludge and characterized. Ochrobactrum tritici was selected for the biodegradation of PVK with high efficiency. To enhance its PVK degradation ability, a whole-cell biodegradation process was established and optimized using Ochrobactrum tritici. The degradation rate with 0.5 mg/ml PVK reached 100% within 3 h. The potential biodegradation pathway was also investigated. To the best of our knowledge, the present study provides new insights into the biodegradation of PVK using an Ochrobactrum tritici strain, a promising candidate strain for the industrial biodegradation of β-lactam antibiotics.
format	article
author	Peng Wang Chen Shen Kaili Xu Qinqin Cong Zhe Dong Luwei Li Jinfeng Guo Jialin Lu Shouxin Liu
author_facet	Peng Wang Chen Shen Kaili Xu Qinqin Cong Zhe Dong Luwei Li Jinfeng Guo Jialin Lu Shouxin Liu
author_sort	Peng Wang
title	Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
title_short	Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
title_full	Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
title_fullStr	Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
title_full_unstemmed	Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation
title_sort	isolation and characterization of <named-content content-type="genus-species">ochrobactrum tritici</named-content> for penicillin v potassium degradation
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/98aab3eaf39e42e29a61509744f0a038
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Isolation and Characterization of <named-content content-type="genus-species">Ochrobactrum tritici</named-content> for Penicillin V Potassium Degradation

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