Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.

It is now commonly accepted that the intestinal microbiota plays a crucial role in the gut physiology and homeostasis, and that both qualitative and quantitative alterations in the compositions of the gut flora exert profound effects on the host's intestinal cells. In spite of this, the details...

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Autores principales:	Blanda Di Luccia, Nicola Manzo, Loredana Baccigalupi, Viola Calabrò, Elvira Crescenzi, Ezio Ricca, Alessandra Pollice
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98b46e40b0104ad1b8c700de8d41891a
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spelling	oai:doaj.org-article:98b46e40b0104ad1b8c700de8d41891a2021-11-18T09:03:32ZLactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.1932-620310.1371/journal.pone.0069102https://doaj.org/article/98b46e40b0104ad1b8c700de8d41891a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23894414/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203It is now commonly accepted that the intestinal microbiota plays a crucial role in the gut physiology and homeostasis, and that both qualitative and quantitative alterations in the compositions of the gut flora exert profound effects on the host's intestinal cells. In spite of this, the details of the interaction between commensal bacteria and intestinal cells are still largely unknown and only in few cases the molecular mechanisms have been elucidated. Here we analyze the effects of molecules produced and secreted by Lactobacillus gasseri SF1183 on human intestinal HCT116 cells. L. gasseri is a well known species of lactic acid bacteria, commonly associated to the human intestine and SF1183 is a human strain previously isolated from an ileal biopsy of an healthy volunteer. SF1183 produces and secretes, in a growth phase-dependent way, molecule(s) able to drastically interfere with HCT116 cell proliferation. Although several attempts to purify and identify the bioactive molecule(s) have been so far unsuccessful, a partial characterization has indicated that it is smaller than 3 kDa, thermostable and of proteinaceous nature. L. gasseri molecule(s) stimulate a G1-phase arrest of the cell cycle by up-regulation of p21WAF1 rendering cells protected from intrinsic and extrinsic apoptosis. A L. gasseri-mediated reduction of apoptosis and of cell proliferation could be relevant in protecting epithelial barrier integrity and helping in reconstituting tissutal homeostasis.Blanda Di LucciaNicola ManzoLoredana BaccigalupiViola CalabròElvira CrescenziEzio RiccaAlessandra PollicePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69102 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Blanda Di Luccia Nicola Manzo Loredana Baccigalupi Viola Calabrò Elvira Crescenzi Ezio Ricca Alessandra Pollice Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
description	It is now commonly accepted that the intestinal microbiota plays a crucial role in the gut physiology and homeostasis, and that both qualitative and quantitative alterations in the compositions of the gut flora exert profound effects on the host's intestinal cells. In spite of this, the details of the interaction between commensal bacteria and intestinal cells are still largely unknown and only in few cases the molecular mechanisms have been elucidated. Here we analyze the effects of molecules produced and secreted by Lactobacillus gasseri SF1183 on human intestinal HCT116 cells. L. gasseri is a well known species of lactic acid bacteria, commonly associated to the human intestine and SF1183 is a human strain previously isolated from an ileal biopsy of an healthy volunteer. SF1183 produces and secretes, in a growth phase-dependent way, molecule(s) able to drastically interfere with HCT116 cell proliferation. Although several attempts to purify and identify the bioactive molecule(s) have been so far unsuccessful, a partial characterization has indicated that it is smaller than 3 kDa, thermostable and of proteinaceous nature. L. gasseri molecule(s) stimulate a G1-phase arrest of the cell cycle by up-regulation of p21WAF1 rendering cells protected from intrinsic and extrinsic apoptosis. A L. gasseri-mediated reduction of apoptosis and of cell proliferation could be relevant in protecting epithelial barrier integrity and helping in reconstituting tissutal homeostasis.
format	article
author	Blanda Di Luccia Nicola Manzo Loredana Baccigalupi Viola Calabrò Elvira Crescenzi Ezio Ricca Alessandra Pollice
author_facet	Blanda Di Luccia Nicola Manzo Loredana Baccigalupi Viola Calabrò Elvira Crescenzi Ezio Ricca Alessandra Pollice
author_sort	Blanda Di Luccia
title	Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
title_short	Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
title_full	Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
title_fullStr	Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
title_full_unstemmed	Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.
title_sort	lactobacillus gasseri sf1183 affects intestinal epithelial cell survival and growth.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/98b46e40b0104ad1b8c700de8d41891a
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Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth.

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