Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples

Abstract Many emerging technologies are reliant on circulating cell-free DNA (cfDNA) and cell-free RNA (cfRNA) applications in the clinic. However, the impact of diurnal cycles or daily meals on circulating analytes are poorly understood and may be confounding factors when developing diagnostic plat...

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Autores principales:	Josiah T. Wagner, Hyun Ji Kim, Katie C. Johnson-Camacho, Taylor Kelley, Laura F. Newell, Paul T. Spellman, Thuy T. M. Ngo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98bffb7158944ae683a9bed573a1b3d2
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spelling	oai:doaj.org-article:98bffb7158944ae683a9bed573a1b3d22021-12-02T18:37:08ZDiurnal stability of cell-free DNA and cell-free RNA in human plasma samples10.1038/s41598-020-73350-32045-2322https://doaj.org/article/98bffb7158944ae683a9bed573a1b3d22020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73350-3https://doaj.org/toc/2045-2322Abstract Many emerging technologies are reliant on circulating cell-free DNA (cfDNA) and cell-free RNA (cfRNA) applications in the clinic. However, the impact of diurnal cycles or daily meals on circulating analytes are poorly understood and may be confounding factors when developing diagnostic platforms. To begin addressing this knowledge gap, we obtained plasma from four healthy donors serially sampled five times during 12 h in a single day. For all samples, we measured concentrations of cfDNA and cfRNA using both bulk measurements and gene-specific digital droplet PCR. We found no significant variation attributed to blood draw number for the cfDNA or cfRNA. This indicated that natural diurnal cycles and meal consumption do not appear to significantly affect abundance of total cfDNA, total cfRNA, or our two selected cfRNA transcripts. Conversely, we observed significant variation between individual donors for cfDNA and one of the cfRNA transcripts. The results of this work suggest that it will be important to consider patient-specific baselines when designing reliable circulating cfDNA or cfRNA clinical assays.Josiah T. WagnerHyun Ji KimKatie C. Johnson-CamachoTaylor KelleyLaura F. NewellPaul T. SpellmanThuy T. M. NgoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Josiah T. Wagner Hyun Ji Kim Katie C. Johnson-Camacho Taylor Kelley Laura F. Newell Paul T. Spellman Thuy T. M. Ngo Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
description	Abstract Many emerging technologies are reliant on circulating cell-free DNA (cfDNA) and cell-free RNA (cfRNA) applications in the clinic. However, the impact of diurnal cycles or daily meals on circulating analytes are poorly understood and may be confounding factors when developing diagnostic platforms. To begin addressing this knowledge gap, we obtained plasma from four healthy donors serially sampled five times during 12 h in a single day. For all samples, we measured concentrations of cfDNA and cfRNA using both bulk measurements and gene-specific digital droplet PCR. We found no significant variation attributed to blood draw number for the cfDNA or cfRNA. This indicated that natural diurnal cycles and meal consumption do not appear to significantly affect abundance of total cfDNA, total cfRNA, or our two selected cfRNA transcripts. Conversely, we observed significant variation between individual donors for cfDNA and one of the cfRNA transcripts. The results of this work suggest that it will be important to consider patient-specific baselines when designing reliable circulating cfDNA or cfRNA clinical assays.
format	article
author	Josiah T. Wagner Hyun Ji Kim Katie C. Johnson-Camacho Taylor Kelley Laura F. Newell Paul T. Spellman Thuy T. M. Ngo
author_facet	Josiah T. Wagner Hyun Ji Kim Katie C. Johnson-Camacho Taylor Kelley Laura F. Newell Paul T. Spellman Thuy T. M. Ngo
author_sort	Josiah T. Wagner
title	Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
title_short	Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
title_full	Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
title_fullStr	Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
title_full_unstemmed	Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples
title_sort	diurnal stability of cell-free dna and cell-free rna in human plasma samples
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/98bffb7158944ae683a9bed573a1b3d2
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Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples

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