Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures

Abstract Genetic, transcriptional, and morphological differences have been reported in pancreatic ductal adenocarcinoma (PDAC) cases. We recently found that epithelial or mesenchymal features were enhanced in three-dimensional (3D) cultures compared to two-dimensional (2D) cultures. In this study, w...

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Autores principales:	Fuuka Minami, Norihiko Sasaki, Yuuki Shichi, Fujiya Gomi, Masaki Michishita, Kozo Ohkusu-Tsukada, Masashi Toyoda, Kimimasa Takahashi, Toshiyuki Ishiwata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98c8eef5297c4fc69d1fbd8dca4302e7
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spelling	oai:doaj.org-article:98c8eef5297c4fc69d1fbd8dca4302e72021-12-02T16:36:05ZMorphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures10.1038/s41598-021-86028-12045-2322https://doaj.org/article/98c8eef5297c4fc69d1fbd8dca4302e72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86028-1https://doaj.org/toc/2045-2322Abstract Genetic, transcriptional, and morphological differences have been reported in pancreatic ductal adenocarcinoma (PDAC) cases. We recently found that epithelial or mesenchymal features were enhanced in three-dimensional (3D) cultures compared to two-dimensional (2D) cultures. In this study, we examined the differences in the morphological and functional characteristics of eight PDAC cell lines in 2D and 3D cultures. Most PDAC cells showed similar pleomorphic morphologies in 2D culture. Under 3D culture, PDAC cells with high E-cadherin and low vimentin expression levels (epithelial) formed small round spheres encircled with flat lining cells, whereas those with high vimentin and low E-cadherin expression levels (mesenchymal) formed large grape-like spheres without lining cells and were highly proliferative. In 3D culture, gemcitabine was more effective for the spheres formed by PDAC cells with epithelial features, while abraxane was more effective on those with mesenchymal features. The expression levels of drug transporters were highest PDAC cells with high vimentin expression levels. These findings indicate that PDAC cells possess various levels of epithelial and mesenchymal characteristics. The 3D-culture method is useful for investigating the diversity of PDAC cell lines and may play important roles in the development of personalized early diagnostic methods and anticancer drugs for PDAC.Fuuka MinamiNorihiko SasakiYuuki ShichiFujiya GomiMasaki MichishitaKozo Ohkusu-TsukadaMasashi ToyodaKimimasa TakahashiToshiyuki IshiwataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fuuka Minami Norihiko Sasaki Yuuki Shichi Fujiya Gomi Masaki Michishita Kozo Ohkusu-Tsukada Masashi Toyoda Kimimasa Takahashi Toshiyuki Ishiwata Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
description	Abstract Genetic, transcriptional, and morphological differences have been reported in pancreatic ductal adenocarcinoma (PDAC) cases. We recently found that epithelial or mesenchymal features were enhanced in three-dimensional (3D) cultures compared to two-dimensional (2D) cultures. In this study, we examined the differences in the morphological and functional characteristics of eight PDAC cell lines in 2D and 3D cultures. Most PDAC cells showed similar pleomorphic morphologies in 2D culture. Under 3D culture, PDAC cells with high E-cadherin and low vimentin expression levels (epithelial) formed small round spheres encircled with flat lining cells, whereas those with high vimentin and low E-cadherin expression levels (mesenchymal) formed large grape-like spheres without lining cells and were highly proliferative. In 3D culture, gemcitabine was more effective for the spheres formed by PDAC cells with epithelial features, while abraxane was more effective on those with mesenchymal features. The expression levels of drug transporters were highest PDAC cells with high vimentin expression levels. These findings indicate that PDAC cells possess various levels of epithelial and mesenchymal characteristics. The 3D-culture method is useful for investigating the diversity of PDAC cell lines and may play important roles in the development of personalized early diagnostic methods and anticancer drugs for PDAC.
format	article
author	Fuuka Minami Norihiko Sasaki Yuuki Shichi Fujiya Gomi Masaki Michishita Kozo Ohkusu-Tsukada Masashi Toyoda Kimimasa Takahashi Toshiyuki Ishiwata
author_facet	Fuuka Minami Norihiko Sasaki Yuuki Shichi Fujiya Gomi Masaki Michishita Kozo Ohkusu-Tsukada Masashi Toyoda Kimimasa Takahashi Toshiyuki Ishiwata
author_sort	Fuuka Minami
title	Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
title_short	Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
title_full	Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
title_fullStr	Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
title_full_unstemmed	Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
title_sort	morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/98c8eef5297c4fc69d1fbd8dca4302e7
work_keys_str_mv	AT fuukaminami morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT norihikosasaki morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT yuukishichi morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT fujiyagomi morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT masakimichishita morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT kozoohkusutsukada morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT masashitoyoda morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT kimimasatakahashi morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures AT toshiyukiishiwata morphofunctionalanalysisofhumanpancreaticcancercelllinesin2and3dimensionalcultures
_version_	1718383646674518016

Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures

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