Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand

Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MD...

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Autores principales:	Chommanad Lerdkrai, Nuch Phungphosop
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	herding dog breeds multi-drug resistance 1 gene mutation p-glycoprotein thai ridgeback dogs Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/98c98faf98f94212b6e46233aa4f1ae4
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spelling	oai:doaj.org-article:98c98faf98f94212b6e46233aa4f1ae42021-11-27T13:03:25ZPrevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand10.14202/vetworld.2021.3015-30200972-89882231-0916https://doaj.org/article/98c98faf98f94212b6e46233aa4f1ae42021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/23.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MDR1 gene mutations have been identified globally, especially in dogs from the USA and European countries. This study aimed to investigate the prevalence of MDR1 nt230(del4) in herding dog breeds and Thai Ridgebacks in Thailand. Materials and Methods: We clarified the prevalence of MDR1 nt230(del4) in 263 dogs of eight purebred dog breeds in Thailand using an allele-specific multiplex polymerase chain reaction method and direct DNA sequencing. Results: Rough Collies, Australian Shepherds, Shetland Sheepdogs, and Old English Sheepdogs were affected by the mutation with mutant allelic frequencies of 57.14%, 12.82%, 11.28%, and 8.33%, respectively. Among these populations, the prevalence of the MDR1 (+/–) genotype was 57.14% (12/21) for Rough Collies, 25.64% (10/39) for Australian Shepherds, 16.13% (15/93) for Shetland Sheepdogs, and 16.67% (2/12) for Old English Sheepdogs, whereas the MDR1 (–/–) mutation was only identified in Rough Collies and Shetland Sheepdogs, with prevalences of 28.57% (6/21) and 3.22% (3/93), respectively. However, the MDR1 nt230(del4) was not identified in Border Collies, German Shepherds, White Swiss Shepherds, or Thai Ridgebacks. Conclusion: This study provides the current situation regarding MDR1 nt230(del4) in herding dog breeds in Thailand. In this survey, we investigated for the first time the status of MDR1 genotype in Thai Ridgebacks. These results are helpful for veterinarians managing effective therapeutic plans for commonly affected dog breeds, and these results will encourage all breeders to improve their selective breeding programs based on the MDR1 nt230(del4) status.Chommanad LerdkraiNuch PhungphosopVeterinary Worldarticleherding dog breedsmulti-drug resistance 1 genemutationp-glycoproteinthai ridgeback dogsAnimal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 3015-3020 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	herding dog breeds multi-drug resistance 1 gene mutation p-glycoprotein thai ridgeback dogs Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	herding dog breeds multi-drug resistance 1 gene mutation p-glycoprotein thai ridgeback dogs Animal culture SF1-1100 Veterinary medicine SF600-1100 Chommanad Lerdkrai Nuch Phungphosop Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
description	Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MDR1 gene mutations have been identified globally, especially in dogs from the USA and European countries. This study aimed to investigate the prevalence of MDR1 nt230(del4) in herding dog breeds and Thai Ridgebacks in Thailand. Materials and Methods: We clarified the prevalence of MDR1 nt230(del4) in 263 dogs of eight purebred dog breeds in Thailand using an allele-specific multiplex polymerase chain reaction method and direct DNA sequencing. Results: Rough Collies, Australian Shepherds, Shetland Sheepdogs, and Old English Sheepdogs were affected by the mutation with mutant allelic frequencies of 57.14%, 12.82%, 11.28%, and 8.33%, respectively. Among these populations, the prevalence of the MDR1 (+/–) genotype was 57.14% (12/21) for Rough Collies, 25.64% (10/39) for Australian Shepherds, 16.13% (15/93) for Shetland Sheepdogs, and 16.67% (2/12) for Old English Sheepdogs, whereas the MDR1 (–/–) mutation was only identified in Rough Collies and Shetland Sheepdogs, with prevalences of 28.57% (6/21) and 3.22% (3/93), respectively. However, the MDR1 nt230(del4) was not identified in Border Collies, German Shepherds, White Swiss Shepherds, or Thai Ridgebacks. Conclusion: This study provides the current situation regarding MDR1 nt230(del4) in herding dog breeds in Thailand. In this survey, we investigated for the first time the status of MDR1 genotype in Thai Ridgebacks. These results are helpful for veterinarians managing effective therapeutic plans for commonly affected dog breeds, and these results will encourage all breeders to improve their selective breeding programs based on the MDR1 nt230(del4) status.
format	article
author	Chommanad Lerdkrai Nuch Phungphosop
author_facet	Chommanad Lerdkrai Nuch Phungphosop
author_sort	Chommanad Lerdkrai
title	Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
title_short	Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
title_full	Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
title_fullStr	Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
title_full_unstemmed	Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
title_sort	prevalence of the mdr1 gene mutation in herding dog breeds and thai ridgebacks in thailand
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/98c98faf98f94212b6e46233aa4f1ae4
work_keys_str_mv	AT chommanadlerdkrai prevalenceofthemdr1genemutationinherdingdogbreedsandthairidgebacksinthailand AT nuchphungphosop prevalenceofthemdr1genemutationinherdingdogbreedsandthairidgebacksinthailand
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Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand

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