Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.

Sorcin, a protein overexpressed in many multi-drug resistant cancers, dynamically localizes to distinct subcellular sites in 3T3-L1 fibroblasts during cell-cycle progression. During interphase sorcin is in the nucleus, in the plasma membrane, in endoplasmic reticulum (ER) cisternae, and in ER-derive...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vasiliki S Lalioti, Andrea Ilari, David J O'Connell, Elena Poser, Ignacio V Sandoval, Gianni Colotti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98ccc40e4dd24fc0aa07dcae20e8ae3a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98ccc40e4dd24fc0aa07dcae20e8ae3a
record_format	dspace
spelling	oai:doaj.org-article:98ccc40e4dd24fc0aa07dcae20e8ae3a2021-11-18T08:38:00ZSorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.1932-620310.1371/journal.pone.0085438https://doaj.org/article/98ccc40e4dd24fc0aa07dcae20e8ae3a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24427308/?tool=EBIhttps://doaj.org/toc/1932-6203Sorcin, a protein overexpressed in many multi-drug resistant cancers, dynamically localizes to distinct subcellular sites in 3T3-L1 fibroblasts during cell-cycle progression. During interphase sorcin is in the nucleus, in the plasma membrane, in endoplasmic reticulum (ER) cisternae, and in ER-derived vesicles localized along the microtubules. These vesicles are positive to RyR, SERCA, calreticulin and Rab10. At the beginning of mitosis, sorcin-containing vesicles associate with the mitotic spindle, and during telophase are concentrated in the cleavage furrow and, subsequently, in the midbody. Sorcin regulates dimensions and calcium load of the ER vesicles by inhibiting RYR and activating SERCA. Analysis of sorcin interactome reveals calcium-dependent interactions with many proteins, including Polo-like kinase 1 (PLK1), Aurora A and Aurora B kinases. Sorcin interacts physically with PLK1, is phosphorylated by PLK1 and induces PLK1 autophosphorylation, thereby regulating kinase activity. Knockdown of sorcin results in major defects in mitosis and cytokinesis, increase in the number of rounded polynucleated cells, blockage of cell progression in G2/M, apoptosis and cell death. Sorcin regulates calcium homeostasis and is necessary for the activation of mitosis and cytokinesis.Vasiliki S LaliotiAndrea IlariDavid J O'ConnellElena PoserIgnacio V SandovalGianni ColottiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85438 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vasiliki S Lalioti Andrea Ilari David J O'Connell Elena Poser Ignacio V Sandoval Gianni Colotti Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
description	Sorcin, a protein overexpressed in many multi-drug resistant cancers, dynamically localizes to distinct subcellular sites in 3T3-L1 fibroblasts during cell-cycle progression. During interphase sorcin is in the nucleus, in the plasma membrane, in endoplasmic reticulum (ER) cisternae, and in ER-derived vesicles localized along the microtubules. These vesicles are positive to RyR, SERCA, calreticulin and Rab10. At the beginning of mitosis, sorcin-containing vesicles associate with the mitotic spindle, and during telophase are concentrated in the cleavage furrow and, subsequently, in the midbody. Sorcin regulates dimensions and calcium load of the ER vesicles by inhibiting RYR and activating SERCA. Analysis of sorcin interactome reveals calcium-dependent interactions with many proteins, including Polo-like kinase 1 (PLK1), Aurora A and Aurora B kinases. Sorcin interacts physically with PLK1, is phosphorylated by PLK1 and induces PLK1 autophosphorylation, thereby regulating kinase activity. Knockdown of sorcin results in major defects in mitosis and cytokinesis, increase in the number of rounded polynucleated cells, blockage of cell progression in G2/M, apoptosis and cell death. Sorcin regulates calcium homeostasis and is necessary for the activation of mitosis and cytokinesis.
format	article
author	Vasiliki S Lalioti Andrea Ilari David J O'Connell Elena Poser Ignacio V Sandoval Gianni Colotti
author_facet	Vasiliki S Lalioti Andrea Ilari David J O'Connell Elena Poser Ignacio V Sandoval Gianni Colotti
author_sort	Vasiliki S Lalioti
title	Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
title_short	Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
title_full	Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
title_fullStr	Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
title_full_unstemmed	Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
title_sort	sorcin links calcium signaling to vesicle trafficking, regulates polo-like kinase 1 and is necessary for mitosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/98ccc40e4dd24fc0aa07dcae20e8ae3a
work_keys_str_mv	AT vasilikislalioti sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis AT andreailari sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis AT davidjoconnell sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis AT elenaposer sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis AT ignaciovsandoval sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis AT giannicolotti sorcinlinkscalciumsignalingtovesicletraffickingregulatespololikekinase1andisnecessaryformitosis
_version_	1718421515841568768

Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.

Ejemplares similares