Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]

Background: A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. Mortierella is one of the significant genera in this field and contains about hundred species. Moreover, M. alpina is the main si...

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Autores principales:	Fuzia Elfituri Muftah Eltariki, Kartikeya Tiwari, Mohammed Abdelfatah Alhoot
Formato:	article
Lenguaje:	EN
Publicado:	F1000 Research Ltd 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98cd46162bd946c3aa1052a63743c3b1
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spelling	oai:doaj.org-article:98cd46162bd946c3aa1052a63743c3b12021-11-08T10:53:47ZMolecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]2046-140210.12688/f1000research.70644.1https://doaj.org/article/98cd46162bd946c3aa1052a63743c3b12021-09-01T00:00:00Zhttps://f1000research.com/articles/10-895/v1https://doaj.org/toc/2046-1402Background: A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. Mortierella is one of the significant genera in this field and contains about hundred species. Moreover, M. alpina is the main single cell oil producer at commercial scale under this genus. Methods: Soil samples from four unique locations of North-East Libya were collected for the isolation of oleaginous Mortierella alpina strains by a serial dilution method. Morphological identification was carried out using light microscopy (Olympus, Japan) and genetic diversity of the isolated Mortierella alpina strains was assessed using conserved internal transcribed spacer (ITS) gene sequences available on the NCBI GenBank database for the confirmation of novelty. The nucleotide sequences reported in this study have been deposited at GenBank (accession no. MZ298831:MZ298835). The MultAlin program was used to align the sequences of closely related strains. The DNA sequences were analyzed for phylogenetic relationships by molecular evolutionary genetic analysis using MEGA X software consisting of Clustal_X v.2.1 for multiple sequence alignment. The neighbour-joining tree was constructed using the Kimura 2-parameter substitution model. Results: The present research study confirms four oleaginous fungal isolates from Libyan soil. These isolates (barcoded as MSU-101, MSU-201, MSU-401 and MSU-501) were discovered and reported for the first time from diverse soil samples of district Aljabal Al-Akhdar in North-East Libya and fall in the class: Zygomycetes; order: Mortierellales. Conclusions: Four oleaginous fungal isolates barcoded as MSU-101, MSU-201, MSU-401 and MSU-501 were identified and confirmed by morphological and molecular analysis. These fungal isolates showed highest similarity with Mortierella alpina species and can be potentialistic single cell oil producers. Thus, the present research study provides insight to the unseen fungal diversity and contributes to more comprehensive Mortierella alpina reference collections worldwide.Fuzia Elfituri Muftah EltarikiKartikeya TiwariMohammed Abdelfatah AlhootF1000 Research LtdarticleMedicineRScienceQENF1000Research, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fuzia Elfituri Muftah Eltariki Kartikeya Tiwari Mohammed Abdelfatah Alhoot Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
description	Background: A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. Mortierella is one of the significant genera in this field and contains about hundred species. Moreover, M. alpina is the main single cell oil producer at commercial scale under this genus. Methods: Soil samples from four unique locations of North-East Libya were collected for the isolation of oleaginous Mortierella alpina strains by a serial dilution method. Morphological identification was carried out using light microscopy (Olympus, Japan) and genetic diversity of the isolated Mortierella alpina strains was assessed using conserved internal transcribed spacer (ITS) gene sequences available on the NCBI GenBank database for the confirmation of novelty. The nucleotide sequences reported in this study have been deposited at GenBank (accession no. MZ298831:MZ298835). The MultAlin program was used to align the sequences of closely related strains. The DNA sequences were analyzed for phylogenetic relationships by molecular evolutionary genetic analysis using MEGA X software consisting of Clustal_X v.2.1 for multiple sequence alignment. The neighbour-joining tree was constructed using the Kimura 2-parameter substitution model. Results: The present research study confirms four oleaginous fungal isolates from Libyan soil. These isolates (barcoded as MSU-101, MSU-201, MSU-401 and MSU-501) were discovered and reported for the first time from diverse soil samples of district Aljabal Al-Akhdar in North-East Libya and fall in the class: Zygomycetes; order: Mortierellales. Conclusions: Four oleaginous fungal isolates barcoded as MSU-101, MSU-201, MSU-401 and MSU-501 were identified and confirmed by morphological and molecular analysis. These fungal isolates showed highest similarity with Mortierella alpina species and can be potentialistic single cell oil producers. Thus, the present research study provides insight to the unseen fungal diversity and contributes to more comprehensive Mortierella alpina reference collections worldwide.
format	article
author	Fuzia Elfituri Muftah Eltariki Kartikeya Tiwari Mohammed Abdelfatah Alhoot
author_facet	Fuzia Elfituri Muftah Eltariki Kartikeya Tiwari Mohammed Abdelfatah Alhoot
author_sort	Fuzia Elfituri Muftah Eltariki
title	Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
title_short	Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
title_full	Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
title_fullStr	Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
title_full_unstemmed	Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]
title_sort	molecular characterization and genetic diversity of four undescribed novel oleaginous mortierella alpina strains from libya [version 1; peer review: 3 approved]
publisher	F1000 Research Ltd
publishDate	2021
url	https://doaj.org/article/98cd46162bd946c3aa1052a63743c3b1
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Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya [version 1; peer review: 3 approved]

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