Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers

Di Chuan,* Yuelong Wang,* Rangrang Fan, Liangxue Zhou, Haifeng Chen, Jianguo Xu, Gang Guo State Key Laboratory of Biotherapy and Cancer Center and Department of Neurosurgery, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, People&rsquo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chuan D, Wang Y, Fan R, Zhou L, Chen H, Xu J, Guo G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	poly(lactic acid) dura matter substitute stereocomplex crystallites electrospinning Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/98cd8626a32e49618c132ca2b3d1ad8c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98cd8626a32e49618c132ca2b3d1ad8c
record_format	dspace
spelling	oai:doaj.org-article:98cd8626a32e49618c132ca2b3d1ad8c2021-12-02T12:35:54ZFabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers1178-2013https://doaj.org/article/98cd8626a32e49618c132ca2b3d1ad8c2020-05-01T00:00:00Zhttps://www.dovepress.com/fabrication-and-properties-of-a-biomimetic-dura-matter-substitute-base-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Di Chuan,* Yuelong Wang,* Rangrang Fan, Liangxue Zhou, Haifeng Chen, Jianguo Xu, Gang Guo State Key Laboratory of Biotherapy and Cancer Center and Department of Neurosurgery, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gang Guo; Liangxue ZhouState Key Laboratory of Biotherapy and Cancer Center, and Department of Neurosurgery, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No. 17, Block 3, Southern Renmin Road, Chengdu 610041, People’s Republic of ChinaTel +86 28-8516 4063Fax +86 28 85164060Email guogang@scu.edu.cn; liangxue_zhou@126.comBackground: Duraplasty is one of the most critical issues in neurosurgical procedures because the defect of dura matter will cause many complications. Electrospinning can mimic the 3D structure of the natural extracellular matrix whose structure is similar to that of dura matter. Poly(L-lactic acid) (PLLA) has been used to fabricate dura matter substitutes and showed compatibility to dural tissue. However, the mechanical properties of the PLLA substitute cannot match the mechanical properties of the human dura mater.Methods and Results: We prepared stereocomplex nanofiber membranes based on enantiomeric poly(lactic acid) and poly(D-lactic acid)-grafted tetracalcium phosphate via electrospinning. X-ray diffraction results showed the formation of stereocomplex crystallites (SC) in the composite nanofiber membranes. Scanning electron microscope observation images showed that composites nanofibers with higher SC formation can keep its original morphologies after heat treatment, suggesting the heat resistance of composite nanofiber membranes. Differential scanning calorimeter tests confirmed that the melting temperature of composite nanofiber membranes was approximately 222°C, higher than that of PLLA. Tensile testing indicated that the ultimate tensile strength and the elongation break of the stereocomplex nanofiber membranes were close to human dura matter. In vitro cytotoxicity studies proved that the stereocomplex nanofiber membranes were non-toxic. The neuron-like differentiation of marrow stem cells on the stereocomplex nanofiber membranes indicated its neuron compatibility.Conclusion: The stereocomplex nanofiber membranes have the potential to serve as a dura mater substitute.Keywords: poly(lactic acid), dura matter substitute, stereocomplex crystallites, electrospinningChuan DWang YFan RZhou LChen HXu JGuo GDove Medical Pressarticlepoly(lactic acid)dura matter substitutestereocomplex crystalliteselectrospinningMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3729-3740 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	poly(lactic acid) dura matter substitute stereocomplex crystallites electrospinning Medicine (General) R5-920
spellingShingle	poly(lactic acid) dura matter substitute stereocomplex crystallites electrospinning Medicine (General) R5-920 Chuan D Wang Y Fan R Zhou L Chen H Xu J Guo G Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
description	Di Chuan,* Yuelong Wang,* Rangrang Fan, Liangxue Zhou, Haifeng Chen, Jianguo Xu, Gang Guo State Key Laboratory of Biotherapy and Cancer Center and Department of Neurosurgery, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gang Guo; Liangxue ZhouState Key Laboratory of Biotherapy and Cancer Center, and Department of Neurosurgery, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No. 17, Block 3, Southern Renmin Road, Chengdu 610041, People’s Republic of ChinaTel +86 28-8516 4063Fax +86 28 85164060Email guogang@scu.edu.cn; liangxue_zhou@126.comBackground: Duraplasty is one of the most critical issues in neurosurgical procedures because the defect of dura matter will cause many complications. Electrospinning can mimic the 3D structure of the natural extracellular matrix whose structure is similar to that of dura matter. Poly(L-lactic acid) (PLLA) has been used to fabricate dura matter substitutes and showed compatibility to dural tissue. However, the mechanical properties of the PLLA substitute cannot match the mechanical properties of the human dura mater.Methods and Results: We prepared stereocomplex nanofiber membranes based on enantiomeric poly(lactic acid) and poly(D-lactic acid)-grafted tetracalcium phosphate via electrospinning. X-ray diffraction results showed the formation of stereocomplex crystallites (SC) in the composite nanofiber membranes. Scanning electron microscope observation images showed that composites nanofibers with higher SC formation can keep its original morphologies after heat treatment, suggesting the heat resistance of composite nanofiber membranes. Differential scanning calorimeter tests confirmed that the melting temperature of composite nanofiber membranes was approximately 222°C, higher than that of PLLA. Tensile testing indicated that the ultimate tensile strength and the elongation break of the stereocomplex nanofiber membranes were close to human dura matter. In vitro cytotoxicity studies proved that the stereocomplex nanofiber membranes were non-toxic. The neuron-like differentiation of marrow stem cells on the stereocomplex nanofiber membranes indicated its neuron compatibility.Conclusion: The stereocomplex nanofiber membranes have the potential to serve as a dura mater substitute.Keywords: poly(lactic acid), dura matter substitute, stereocomplex crystallites, electrospinning
format	article
author	Chuan D Wang Y Fan R Zhou L Chen H Xu J Guo G
author_facet	Chuan D Wang Y Fan R Zhou L Chen H Xu J Guo G
author_sort	Chuan D
title	Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
title_short	Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
title_full	Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
title_fullStr	Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
title_full_unstemmed	Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers
title_sort	fabrication and properties of a biomimetic dura matter substitute based on stereocomplex poly(lactic acid) nanofibers
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/98cd8626a32e49618c132ca2b3d1ad8c
work_keys_str_mv	AT chuand fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT wangy fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT fanr fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT zhoul fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT chenh fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT xuj fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers AT guog fabricationandpropertiesofabiomimeticduramattersubstitutebasedonstereocomplexpolylacticacidnanofibers
_version_	1718393814724378624

Fabrication and Properties of a Biomimetic Dura Matter Substitute Based on Stereocomplex Poly(Lactic Acid) Nanofibers

Ejemplares similares