Non-invasive imaging of engineered human tumors in the living chicken embryo

Abstract The growing interest in engineered tumor models prompted us to devise a method for the non-invasive assessment of such models. Here, we report on bioluminescence imaging (BLI) for the assessment of engineered tumor models in the fertilized chicken egg, i.e, chick chorioallantoic membrane (C...

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Autores principales:	Benedict Jefferies, Florian Lenze, Anuja Sathe, Nguyen Truong, Martina Anton, Rüdiger von Eisenhart-Rothe, Roman Nawroth, Philipp Mayer-Kuckuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98d24628b56844d89348bbffb195f2cc
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spelling	oai:doaj.org-article:98d24628b56844d89348bbffb195f2cc2021-12-02T15:06:23ZNon-invasive imaging of engineered human tumors in the living chicken embryo10.1038/s41598-017-04572-12045-2322https://doaj.org/article/98d24628b56844d89348bbffb195f2cc2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04572-1https://doaj.org/toc/2045-2322Abstract The growing interest in engineered tumor models prompted us to devise a method for the non-invasive assessment of such models. Here, we report on bioluminescence imaging (BLI) for the assessment of engineered tumor models in the fertilized chicken egg, i.e, chick chorioallantoic membrane (CAM) assay. One prostate cancer (PC-3) and two osteosarcoma (MG63 and HOS) cell lines were modified with luciferase reporter genes. To create engineered tumors, these cell lines were seeded either onto basement membrane extract (BME) or gelfoam scaffolds, and subsequently grafted in vivo onto the CAM. BLI enabled non-invasive, specific detection of the engineered tumors on the CAM in the living chicken embryo. Further, BLI permitted daily, quantitative monitoring of the engineered tumors over the course of up to 7 days. Data showed that an extracellular matrix (ECM) composed of BME supported growth of reporter gene marked PC-3 tumors but did not support MG63 or HOS tumor growth. However, MG63 tumors engineered on the collagen-based gelfoam ECM showed a temporal proliferation burst in MG63 tumors. Together, the data demonstrated imaging of engineered human cancer models in living chicken embryos. The combination of CAM assay and BLI holds significant potential for the examination of a broad range of engineered tumor models.Benedict JefferiesFlorian LenzeAnuja SatheNguyen TruongMartina AntonRüdiger von Eisenhart-RotheRoman NawrothPhilipp Mayer-KuckukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benedict Jefferies Florian Lenze Anuja Sathe Nguyen Truong Martina Anton Rüdiger von Eisenhart-Rothe Roman Nawroth Philipp Mayer-Kuckuk Non-invasive imaging of engineered human tumors in the living chicken embryo
description	Abstract The growing interest in engineered tumor models prompted us to devise a method for the non-invasive assessment of such models. Here, we report on bioluminescence imaging (BLI) for the assessment of engineered tumor models in the fertilized chicken egg, i.e, chick chorioallantoic membrane (CAM) assay. One prostate cancer (PC-3) and two osteosarcoma (MG63 and HOS) cell lines were modified with luciferase reporter genes. To create engineered tumors, these cell lines were seeded either onto basement membrane extract (BME) or gelfoam scaffolds, and subsequently grafted in vivo onto the CAM. BLI enabled non-invasive, specific detection of the engineered tumors on the CAM in the living chicken embryo. Further, BLI permitted daily, quantitative monitoring of the engineered tumors over the course of up to 7 days. Data showed that an extracellular matrix (ECM) composed of BME supported growth of reporter gene marked PC-3 tumors but did not support MG63 or HOS tumor growth. However, MG63 tumors engineered on the collagen-based gelfoam ECM showed a temporal proliferation burst in MG63 tumors. Together, the data demonstrated imaging of engineered human cancer models in living chicken embryos. The combination of CAM assay and BLI holds significant potential for the examination of a broad range of engineered tumor models.
format	article
author	Benedict Jefferies Florian Lenze Anuja Sathe Nguyen Truong Martina Anton Rüdiger von Eisenhart-Rothe Roman Nawroth Philipp Mayer-Kuckuk
author_facet	Benedict Jefferies Florian Lenze Anuja Sathe Nguyen Truong Martina Anton Rüdiger von Eisenhart-Rothe Roman Nawroth Philipp Mayer-Kuckuk
author_sort	Benedict Jefferies
title	Non-invasive imaging of engineered human tumors in the living chicken embryo
title_short	Non-invasive imaging of engineered human tumors in the living chicken embryo
title_full	Non-invasive imaging of engineered human tumors in the living chicken embryo
title_fullStr	Non-invasive imaging of engineered human tumors in the living chicken embryo
title_full_unstemmed	Non-invasive imaging of engineered human tumors in the living chicken embryo
title_sort	non-invasive imaging of engineered human tumors in the living chicken embryo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/98d24628b56844d89348bbffb195f2cc
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Non-invasive imaging of engineered human tumors in the living chicken embryo

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