ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

BACKGROUND AND OBJECTIVE: Chronic obstructive pulmonary disease (COPD), a lung disease which is characterized by decreased expiratory flow rates, increased pulmonary resistance and hyperinflation. In vitro studies indicate that the activity of cytochrome C oxidase (COX) can be directly regulated by...

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Autores principales:	MS Hakhamaneshi, S.AR Mesbah Namin, , Houshmand, A Sahebghadam Lotfi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2008
Materias:	copd cox mitochondria specific activity Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/98d76561263a4179963b6beb967c0bc3
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spelling	oai:doaj.org-article:98d76561263a4179963b6beb967c0bc32021-11-10T09:07:19ZASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE1561-41072251-7170https://doaj.org/article/98d76561263a4179963b6beb967c0bc32008-04-01T00:00:00Zhttp://jbums.org/article-1-2478-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Chronic obstructive pulmonary disease (COPD), a lung disease which is characterized by decreased expiratory flow rates, increased pulmonary resistance and hyperinflation. In vitro studies indicate that the activity of cytochrome C oxidase (COX) can be directly regulated by the presence of molecular oxygen. The aim of this study was to assess cytochrome C oxidase activity in white cells of patients with chronic obstructive pulmonary disease. METHODS: This case-control study was performed on 42 patients under clinically stable conditions and 50 healthy sedentary volunteers of similar age. Whole blood was collected and white blood cells (WBCs) separated and lysed. The homogenates were centrifuged and the supernatants were used for total protein content, COX and citrate synthase activity. Absolute specific COX activity and relative activities were determined. FINDINGS: Mitochondrial COX activity and specific activity (absolute & relative) in WBCs were significantly increased (about 3.5 time) in patients with COPD in comparison with control samples. CONCLUSION: These results indicate that the activity of COX is increased in WBCs of patients with COPD but whether this is a primary or secondary change relevant to hypoxic condition in these patients is not clear and need further study.MS Hakhamaneshi,S.AR Mesbah Namin,, HoushmandA Sahebghadam LotfiBabol University of Medical Sciencesarticlecopdcoxmitochondriaspecific activityMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 10, Iss 1, Pp 14-19 (2008)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	copd cox mitochondria specific activity Medicine R Medicine (General) R5-920
spellingShingle	copd cox mitochondria specific activity Medicine R Medicine (General) R5-920 MS Hakhamaneshi, S.AR Mesbah Namin, , Houshmand A Sahebghadam Lotfi ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
description	BACKGROUND AND OBJECTIVE: Chronic obstructive pulmonary disease (COPD), a lung disease which is characterized by decreased expiratory flow rates, increased pulmonary resistance and hyperinflation. In vitro studies indicate that the activity of cytochrome C oxidase (COX) can be directly regulated by the presence of molecular oxygen. The aim of this study was to assess cytochrome C oxidase activity in white cells of patients with chronic obstructive pulmonary disease. METHODS: This case-control study was performed on 42 patients under clinically stable conditions and 50 healthy sedentary volunteers of similar age. Whole blood was collected and white blood cells (WBCs) separated and lysed. The homogenates were centrifuged and the supernatants were used for total protein content, COX and citrate synthase activity. Absolute specific COX activity and relative activities were determined. FINDINGS: Mitochondrial COX activity and specific activity (absolute & relative) in WBCs were significantly increased (about 3.5 time) in patients with COPD in comparison with control samples. CONCLUSION: These results indicate that the activity of COX is increased in WBCs of patients with COPD but whether this is a primary or secondary change relevant to hypoxic condition in these patients is not clear and need further study.
format	article
author	MS Hakhamaneshi, S.AR Mesbah Namin, , Houshmand A Sahebghadam Lotfi
author_facet	MS Hakhamaneshi, S.AR Mesbah Namin, , Houshmand A Sahebghadam Lotfi
author_sort	MS Hakhamaneshi,
title	ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_short	ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_full	ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_fullStr	ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_full_unstemmed	ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_sort	assessment of cytochrome c oxidase activity in white blood cells of patients with chronic obstructive pulmonary disease
publisher	Babol University of Medical Sciences
publishDate	2008
url	https://doaj.org/article/98d76561263a4179963b6beb967c0bc3
work_keys_str_mv	AT mshakhamaneshi assessmentofcytochromecoxidaseactivityinwhitebloodcellsofpatientswithchronicobstructivepulmonarydisease AT sarmesbahnamin assessmentofcytochromecoxidaseactivityinwhitebloodcellsofpatientswithchronicobstructivepulmonarydisease AT houshmand assessmentofcytochromecoxidaseactivityinwhitebloodcellsofpatientswithchronicobstructivepulmonarydisease AT asahebghadamlotfi assessmentofcytochromecoxidaseactivityinwhitebloodcellsofpatientswithchronicobstructivepulmonarydisease
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ASSESSMENT OF CYTOCHROME C OXIDASE ACTIVITY IN WHITE BLOOD CELLS OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

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