DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS

Objective: To evaluate the burden of carbapenemases producers among carbapenem-resistant isolates. Study Design: Cross-sectional study. Place and Duration of Study: This cross-sectional study was conducted at microbiology lab, Pathology department, Allama Iqbal Medical College Lahore, from Jul 20...

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Autores principales:	Mariam Awan, Farhan Rasheed, Muhammad Saeed, Shagufta Irum, Fouzia Ashraf, Ambreen Anwar Imran
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	carbapenem carbapenemases healthcare workers Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/98d8aa56777f45ae8e62c7d34d57c0f0
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spelling	oai:doaj.org-article:98d8aa56777f45ae8e62c7d34d57c0f02021-11-15T04:57:11ZDISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS0030-96482411-8842https://doaj.org/article/98d8aa56777f45ae8e62c7d34d57c0f02019-02-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/2486/2043https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To evaluate the burden of carbapenemases producers among carbapenem-resistant isolates. Study Design: Cross-sectional study. Place and Duration of Study: This cross-sectional study was conducted at microbiology lab, Pathology department, Allama Iqbal Medical College Lahore, from Jul 2016 to Apr 2017. Material and Methods: A total of 12126 clinical specimens were enrolled through a non-probability/consecutive sampling technique, every sample was processed for bacterial culture, identification was done on the basis of colonial morphology, Gram stain, and biochemical profile. All those isolates that were Gram-negative rods, were processed for carbapenemases detection by Modified Hodge test (MHT) in accordance with CLSI 2016. Results: Out of total n=12126 samples, culture positive were 35.9% (n=4361) of which 40.5% (n=1770) were Gram-negative rods, among these 9.6% (n=170) were carbapenem-resistant isolates of which 50% (n=85) were carbapanamases producers (MHT positive). Organisms wise carbapenem resistance was detected in Acineto- bacter species 44.%, Pseudomonas species 34%, E.coli 7%, Klebsiella species 8%, Proteus species 3%, Citrobacter species 1%, Enterobacter species 0.7% Among these isolates MHT positivity was as followed Acinetobacter species were 53%, Pseudomonas species 51%, E.coli 36%, Klebsiella species 50%, Proteus species 20%, Citrobacter species 66%, Enterobacter species 50%, department wise distribution of carbapenemases producer showed that among total sample received from surgical units 51.4%, ICU 65.3% medical units 43.5%, pediatric ward 71.4%, orthopedic 11.1% neurosurgery 20%, other wards 55.1% were MHT positive. The almost similar resistant pattern was observed in MHT positive and negative isolates. Conclusion: Emergence of carbapenem resistance is an alarming situation in clinical settings.resuMariam AwanFarhan RasheedMuhammad SaeedShagufta IrumFouzia AshrafAmbreen Anwar ImranArmy Medical College Rawalpindiarticlecarbapenemcarbapenemaseshealthcare workersMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 1, Pp 9-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbapenem carbapenemases healthcare workers Medicine R Medicine (General) R5-920
spellingShingle	carbapenem carbapenemases healthcare workers Medicine R Medicine (General) R5-920 Mariam Awan Farhan Rasheed Muhammad Saeed Shagufta Irum Fouzia Ashraf Ambreen Anwar Imran DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
description	Objective: To evaluate the burden of carbapenemases producers among carbapenem-resistant isolates. Study Design: Cross-sectional study. Place and Duration of Study: This cross-sectional study was conducted at microbiology lab, Pathology department, Allama Iqbal Medical College Lahore, from Jul 2016 to Apr 2017. Material and Methods: A total of 12126 clinical specimens were enrolled through a non-probability/consecutive sampling technique, every sample was processed for bacterial culture, identification was done on the basis of colonial morphology, Gram stain, and biochemical profile. All those isolates that were Gram-negative rods, were processed for carbapenemases detection by Modified Hodge test (MHT) in accordance with CLSI 2016. Results: Out of total n=12126 samples, culture positive were 35.9% (n=4361) of which 40.5% (n=1770) were Gram-negative rods, among these 9.6% (n=170) were carbapenem-resistant isolates of which 50% (n=85) were carbapanamases producers (MHT positive). Organisms wise carbapenem resistance was detected in Acineto- bacter species 44.%, Pseudomonas species 34%, E.coli 7%, Klebsiella species 8%, Proteus species 3%, Citrobacter species 1%, Enterobacter species 0.7% Among these isolates MHT positivity was as followed Acinetobacter species were 53%, Pseudomonas species 51%, E.coli 36%, Klebsiella species 50%, Proteus species 20%, Citrobacter species 66%, Enterobacter species 50%, department wise distribution of carbapenemases producer showed that among total sample received from surgical units 51.4%, ICU 65.3% medical units 43.5%, pediatric ward 71.4%, orthopedic 11.1% neurosurgery 20%, other wards 55.1% were MHT positive. The almost similar resistant pattern was observed in MHT positive and negative isolates. Conclusion: Emergence of carbapenem resistance is an alarming situation in clinical settings.resu
format	article
author	Mariam Awan Farhan Rasheed Muhammad Saeed Shagufta Irum Fouzia Ashraf Ambreen Anwar Imran
author_facet	Mariam Awan Farhan Rasheed Muhammad Saeed Shagufta Irum Fouzia Ashraf Ambreen Anwar Imran
author_sort	Mariam Awan
title	DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
title_short	DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
title_full	DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
title_fullStr	DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
title_full_unstemmed	DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS
title_sort	dissemination and detection of carbapenemases producing gram-negative rods
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/98d8aa56777f45ae8e62c7d34d57c0f0
work_keys_str_mv	AT mariamawan disseminationanddetectionofcarbapenemasesproducinggramnegativerods AT farhanrasheed disseminationanddetectionofcarbapenemasesproducinggramnegativerods AT muhammadsaeed disseminationanddetectionofcarbapenemasesproducinggramnegativerods AT shaguftairum disseminationanddetectionofcarbapenemasesproducinggramnegativerods AT fouziaashraf disseminationanddetectionofcarbapenemasesproducinggramnegativerods AT ambreenanwarimran disseminationanddetectionofcarbapenemasesproducinggramnegativerods
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DISSEMINATION AND DETECTION OF CARBAPENEMASES PRODUCING GRAM-NEGATIVE RODS

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