Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

Wei-Bin Shen,1,2 Celine Plachez,2,3 Amanda Chan,4 Deborah Yarnell,1 Adam C Puche,3 Paul S Fishman,1,5 Paul Yarowsky1,21Research Service, VA Maryland Health Care System, Baltimore, MD, USA; 2Department of Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA; 3Department of Anat...

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Autores principales:	Shen WB, Plachez C, Chan A, Yarnell D, Puche AC, Fishman PS, Yarowsky P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/98dc7bcea2414ae9ba95d5b144f26e16
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spelling	oai:doaj.org-article:98dc7bcea2414ae9ba95d5b144f26e162021-12-02T04:20:57ZHuman neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B1176-91141178-2013https://doaj.org/article/98dc7bcea2414ae9ba95d5b144f26e162013-11-01T00:00:00Zhttp://www.dovepress.com/human-neural-progenitor-cells-retain-viability-phenotype-proliferation-a15121https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Wei-Bin Shen,1,2 Celine Plachez,2,3 Amanda Chan,4 Deborah Yarnell,1 Adam C Puche,3 Paul S Fishman,1,5 Paul Yarowsky1,21Research Service, VA Maryland Health Care System, Baltimore, MD, USA; 2Department of Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA; 3Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA; 4Notre Dame of Maryland School of Pharmacy, Baltimore, MD, USA; 5Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, USAAbstract: Ultrasmall superparamagnetic iron-oxide particles (USPIOs) loaded into stem cells have been suggested as a way to track stem cell transplantation with magnetic resonance imaging, but the labeling, and post-labeling proliferation, viability, differentiation, and retention of USPIOs within the stem cells have yet to be determined for each type of stem cell and for each type of USPIO. Molday ION Rhodamine B™ (BioPAL, Worcester, MA, USA) (MIRB) has been shown to be a USPIO labeling agent for mesenchymal stem cells, glial progenitor cells, and stem cell lines. In this study, we have evaluated MIRB labeling in human neuroprogenitor cells and found that human neuroprogenitor cells are effectively labeled with MIRB without use of transfection reagents. Viability, proliferation, and differentiation properties are unchanged between MIRB-labeled neuroprogenitors cells and unlabeled cells. Moreover, MIRB-labeled human neuroprogenitor cells can be frozen, thawed, and replated without loss of MIRB or even without loss of their intrinsic biology. Overall, those results show that MIRB has advantageous properties that can be used for cell-based therapy.Keywords: ferumoxides, USPIO, MION, neural stem cells, SC121 antibody, human, toxicologyShen WBPlachez CChan AYarnell DPuche ACFishman PSYarowsky PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4593-4600 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shen WB Plachez C Chan A Yarnell D Puche AC Fishman PS Yarowsky P Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
description	Wei-Bin Shen,1,2 Celine Plachez,2,3 Amanda Chan,4 Deborah Yarnell,1 Adam C Puche,3 Paul S Fishman,1,5 Paul Yarowsky1,21Research Service, VA Maryland Health Care System, Baltimore, MD, USA; 2Department of Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA; 3Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA; 4Notre Dame of Maryland School of Pharmacy, Baltimore, MD, USA; 5Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, USAAbstract: Ultrasmall superparamagnetic iron-oxide particles (USPIOs) loaded into stem cells have been suggested as a way to track stem cell transplantation with magnetic resonance imaging, but the labeling, and post-labeling proliferation, viability, differentiation, and retention of USPIOs within the stem cells have yet to be determined for each type of stem cell and for each type of USPIO. Molday ION Rhodamine B™ (BioPAL, Worcester, MA, USA) (MIRB) has been shown to be a USPIO labeling agent for mesenchymal stem cells, glial progenitor cells, and stem cell lines. In this study, we have evaluated MIRB labeling in human neuroprogenitor cells and found that human neuroprogenitor cells are effectively labeled with MIRB without use of transfection reagents. Viability, proliferation, and differentiation properties are unchanged between MIRB-labeled neuroprogenitors cells and unlabeled cells. Moreover, MIRB-labeled human neuroprogenitor cells can be frozen, thawed, and replated without loss of MIRB or even without loss of their intrinsic biology. Overall, those results show that MIRB has advantageous properties that can be used for cell-based therapy.Keywords: ferumoxides, USPIO, MION, neural stem cells, SC121 antibody, human, toxicology
format	article
author	Shen WB Plachez C Chan A Yarnell D Puche AC Fishman PS Yarowsky P
author_facet	Shen WB Plachez C Chan A Yarnell D Puche AC Fishman PS Yarowsky P
author_sort	Shen WB
title	Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
title_short	Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
title_full	Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
title_fullStr	Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
title_full_unstemmed	Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B
title_sort	human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, molday ion rhodamine b
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/98dc7bcea2414ae9ba95d5b144f26e16
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Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

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