The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis

The intrinsic flexibility of glycans complicates the study of their structures and dynamics, which are often important for their biological function. NMR has provided insights into the conformational, dynamic and recognition features of glycans, but suffers from severe chemical shift degeneracy. We...

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Autores principales:	Ana Poveda, Giulio Fittolani, Peter H. Seeberger, Martina Delbianco, Jesús Jiménez-Barbero
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glycans NMR RDC 13C-labelling automated glycan assembly Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/98de83ee4f304507a90349ff696d2cbb
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spelling	oai:doaj.org-article:98de83ee4f304507a90349ff696d2cbb2021-11-10T06:53:29ZThe Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis2296-889X10.3389/fmolb.2021.784318https://doaj.org/article/98de83ee4f304507a90349ff696d2cbb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.784318/fullhttps://doaj.org/toc/2296-889XThe intrinsic flexibility of glycans complicates the study of their structures and dynamics, which are often important for their biological function. NMR has provided insights into the conformational, dynamic and recognition features of glycans, but suffers from severe chemical shift degeneracy. We employed labelled glycans to explore the conformational behaviour of a β(1-6)-Glc hexasaccharide model through residual dipolar couplings (RDCs). RDC delivered information on the relative orientation of specific residues along the glycan chain and provided experimental clues for the existence of certain geometries. The use of two different aligning media demonstrated the adaptability of flexible oligosaccharide structures to different environments.Ana PovedaGiulio FittolaniGiulio FittolaniPeter H. SeebergerPeter H. SeebergerMartina DelbiancoJesús Jiménez-BarberoJesús Jiménez-BarberoJesús Jiménez-BarberoJesús Jiménez-BarberoFrontiers Media S.A.articleglycansNMRRDC13C-labellingautomated glycan assemblyBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycans NMR RDC 13C-labelling automated glycan assembly Biology (General) QH301-705.5
spellingShingle	glycans NMR RDC 13C-labelling automated glycan assembly Biology (General) QH301-705.5 Ana Poveda Giulio Fittolani Giulio Fittolani Peter H. Seeberger Peter H. Seeberger Martina Delbianco Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
description	The intrinsic flexibility of glycans complicates the study of their structures and dynamics, which are often important for their biological function. NMR has provided insights into the conformational, dynamic and recognition features of glycans, but suffers from severe chemical shift degeneracy. We employed labelled glycans to explore the conformational behaviour of a β(1-6)-Glc hexasaccharide model through residual dipolar couplings (RDCs). RDC delivered information on the relative orientation of specific residues along the glycan chain and provided experimental clues for the existence of certain geometries. The use of two different aligning media demonstrated the adaptability of flexible oligosaccharide structures to different environments.
format	article
author	Ana Poveda Giulio Fittolani Giulio Fittolani Peter H. Seeberger Peter H. Seeberger Martina Delbianco Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero
author_facet	Ana Poveda Giulio Fittolani Giulio Fittolani Peter H. Seeberger Peter H. Seeberger Martina Delbianco Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero Jesús Jiménez-Barbero
author_sort	Ana Poveda
title	The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
title_short	The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
title_full	The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
title_fullStr	The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
title_full_unstemmed	The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis
title_sort	flexibility of oligosaccharides unveiled through residual dipolar coupling analysis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/98de83ee4f304507a90349ff696d2cbb
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The Flexibility of Oligosaccharides Unveiled Through Residual Dipolar Coupling Analysis

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