3D cell neighbour dynamics in growing pseudostratified epithelia

During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell seg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Harold Fernando Gómez, Mathilde Sabine Dumond, Leonie Hodel, Roman Vetter, Dagmar Iber
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	epithelial organisation Euler's formula Lewis' law Aboav-Weaire's law physical principles lightsheet microscopy Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/98dedc46931b426191b956caacc72998
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98dedc46931b426191b956caacc72998
record_format	dspace
spelling	oai:doaj.org-article:98dedc46931b426191b956caacc729982021-11-05T12:00:36Z3D cell neighbour dynamics in growing pseudostratified epithelia10.7554/eLife.681352050-084Xe68135https://doaj.org/article/98dedc46931b426191b956caacc729982021-10-01T00:00:00Zhttps://elifesciences.org/articles/68135https://doaj.org/toc/2050-084XDuring morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell segmentation, and physical theory to unravel the principles behind 3D cell organisation in growing pseudostratified epithelia. We find that cells have highly irregular 3D shapes and exhibit numerous neighbour intercalations along the apical-basal axis as well as over time. Despite the fluidic nature, the cell packing configurations follow fundamental relationships previously described for apical epithelial layers, that is, Euler's polyhedron formula, Lewis’ law, and Aboav-Weaire's law, at all times and across the entire tissue thickness. This arrangement minimises the lateral cell-cell surface energy for a given cross-sectional area variability, generated primarily by the distribution and movement of nuclei. We conclude that the complex 3D cell organisation in growing epithelia emerges from simple physical principles.Harold Fernando GómezMathilde Sabine DumondLeonie HodelRoman VetterDagmar IbereLife Sciences Publications Ltdarticleepithelial organisationEuler's formulaLewis' lawAboav-Weaire's lawphysical principleslightsheet microscopyMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	epithelial organisation Euler's formula Lewis' law Aboav-Weaire's law physical principles lightsheet microscopy Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	epithelial organisation Euler's formula Lewis' law Aboav-Weaire's law physical principles lightsheet microscopy Medicine R Science Q Biology (General) QH301-705.5 Harold Fernando Gómez Mathilde Sabine Dumond Leonie Hodel Roman Vetter Dagmar Iber 3D cell neighbour dynamics in growing pseudostratified epithelia
description	During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell segmentation, and physical theory to unravel the principles behind 3D cell organisation in growing pseudostratified epithelia. We find that cells have highly irregular 3D shapes and exhibit numerous neighbour intercalations along the apical-basal axis as well as over time. Despite the fluidic nature, the cell packing configurations follow fundamental relationships previously described for apical epithelial layers, that is, Euler's polyhedron formula, Lewis’ law, and Aboav-Weaire's law, at all times and across the entire tissue thickness. This arrangement minimises the lateral cell-cell surface energy for a given cross-sectional area variability, generated primarily by the distribution and movement of nuclei. We conclude that the complex 3D cell organisation in growing epithelia emerges from simple physical principles.
format	article
author	Harold Fernando Gómez Mathilde Sabine Dumond Leonie Hodel Roman Vetter Dagmar Iber
author_facet	Harold Fernando Gómez Mathilde Sabine Dumond Leonie Hodel Roman Vetter Dagmar Iber
author_sort	Harold Fernando Gómez
title	3D cell neighbour dynamics in growing pseudostratified epithelia
title_short	3D cell neighbour dynamics in growing pseudostratified epithelia
title_full	3D cell neighbour dynamics in growing pseudostratified epithelia
title_fullStr	3D cell neighbour dynamics in growing pseudostratified epithelia
title_full_unstemmed	3D cell neighbour dynamics in growing pseudostratified epithelia
title_sort	3d cell neighbour dynamics in growing pseudostratified epithelia
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/98dedc46931b426191b956caacc72998
work_keys_str_mv	AT haroldfernandogomez 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT mathildesabinedumond 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT leoniehodel 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT romanvetter 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT dagmariber 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia
_version_	1718444303934554112

3D cell neighbour dynamics in growing pseudostratified epithelia

Ejemplares similares