IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES

Immunomodulatory activity of a native antimicrobial peptide indolicidin and its synthetic structural analogues (indolicidin 7, 8,20,21,22), with spatial uncoupling of hydrophobic and hydrophilic parts of the molecule and different net charges was under investigation. It was revealed that indolicidin...

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Autores principales:	A. Yu. Artamonov, S. N. Shanin, D. S. Orlov, O. V. Shamova, N. I. Kolodkin, E. G. Rybakina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	antimicrobial peptides indolicidin structural analogues immunomodulatory activity Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/98dfcd9cccc745a09b6885d01dc93c1b
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spelling	oai:doaj.org-article:98dfcd9cccc745a09b6885d01dc93c1b2021-11-18T08:03:41ZIMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES1563-06252313-741X10.15789/1563-0625-2009-1-101-104https://doaj.org/article/98dfcd9cccc745a09b6885d01dc93c1b2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/516https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XImmunomodulatory activity of a native antimicrobial peptide indolicidin and its synthetic structural analogues (indolicidin 7, 8,20,21,22), with spatial uncoupling of hydrophobic and hydrophilic parts of the molecule and different net charges was under investigation. It was revealed that indolicidin 7 and 22 at concentrations of, respectively, 0.6 uM and 5 uM suppressed cytotoxic activity of natural killer spleen cells against tumor cells (K-562 line), while indolicidins 8,20 and 21 at the concentrations of 1.3 uM, 2.5 uM and 5 uM respectively, did not influence it. It was shown for the first time, that native indolicidin and its structural analogues indolicidin 8 and 20 did not increase activity of cell division, whereas indolicidin 7 and 22 had a direct mitogenic activity on spleen cells. The investigated peptides showed inhibitory effect upon mitogenic transformation of splenocytes induced by Con A. In addition, indolicidin and its structural analogues suppressed co-mitogenic activity of Interleikin-1 towards splenocytes. The results obtained allow of a conclusion that the structural changes of indolicidin molecule lead to emergence of novel immunomodulatory activities of the peptides, whereas the methods of structural modifications demonstrate potential approaches to directed design of molecules with optimal biological properties.A. Yu. ArtamonovS. N. ShaninD. S. OrlovO. V. ShamovaN. I. KolodkinE. G. RybakinaSPb RAACIarticleantimicrobial peptidesindolicidinstructural analoguesimmunomodulatory activityImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 1, Pp 101-104 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	antimicrobial peptides indolicidin structural analogues immunomodulatory activity Immunologic diseases. Allergy RC581-607
spellingShingle	antimicrobial peptides indolicidin structural analogues immunomodulatory activity Immunologic diseases. Allergy RC581-607 A. Yu. Artamonov S. N. Shanin D. S. Orlov O. V. Shamova N. I. Kolodkin E. G. Rybakina IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
description	Immunomodulatory activity of a native antimicrobial peptide indolicidin and its synthetic structural analogues (indolicidin 7, 8,20,21,22), with spatial uncoupling of hydrophobic and hydrophilic parts of the molecule and different net charges was under investigation. It was revealed that indolicidin 7 and 22 at concentrations of, respectively, 0.6 uM and 5 uM suppressed cytotoxic activity of natural killer spleen cells against tumor cells (K-562 line), while indolicidins 8,20 and 21 at the concentrations of 1.3 uM, 2.5 uM and 5 uM respectively, did not influence it. It was shown for the first time, that native indolicidin and its structural analogues indolicidin 8 and 20 did not increase activity of cell division, whereas indolicidin 7 and 22 had a direct mitogenic activity on spleen cells. The investigated peptides showed inhibitory effect upon mitogenic transformation of splenocytes induced by Con A. In addition, indolicidin and its structural analogues suppressed co-mitogenic activity of Interleikin-1 towards splenocytes. The results obtained allow of a conclusion that the structural changes of indolicidin molecule lead to emergence of novel immunomodulatory activities of the peptides, whereas the methods of structural modifications demonstrate potential approaches to directed design of molecules with optimal biological properties.
format	article
author	A. Yu. Artamonov S. N. Shanin D. S. Orlov O. V. Shamova N. I. Kolodkin E. G. Rybakina
author_facet	A. Yu. Artamonov S. N. Shanin D. S. Orlov O. V. Shamova N. I. Kolodkin E. G. Rybakina
author_sort	A. Yu. Artamonov
title	IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
title_short	IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
title_full	IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
title_fullStr	IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
title_full_unstemmed	IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES
title_sort	immunomodulatory activity of antimicrobial peptide indolicidin and its structural analogues
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/98dfcd9cccc745a09b6885d01dc93c1b
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IMMUNOMODULATORY ACTIVITY OF ANTIMICROBIAL PEPTIDE INDOLICIDIN AND ITS STRUCTURAL ANALOGUES

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