Gelsolin-independent podosome formation in dendritic cells.

Podosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filame...

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Autores principales:	Oscar Hammarfjord, Hervé Falet, Christine Gurniak, John H Hartwig, Robert P A Wallin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/990cd1672b7d4f18ab0170464c3c1b4b
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spelling	oai:doaj.org-article:990cd1672b7d4f18ab0170464c3c1b4b2021-11-18T06:50:29ZGelsolin-independent podosome formation in dendritic cells.1932-620310.1371/journal.pone.0021615https://doaj.org/article/990cd1672b7d4f18ab0170464c3c1b4b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779330/?tool=EBIhttps://doaj.org/toc/1932-6203Podosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filament scaffolds onto which proteins important for their function, such as matrix metallo-proteases and integrins, attach. The dynamics of the podosomes require the action of many proteins regulating actin assembly and disassembly. One such protein, gelsolin, which associates to podosomes, has been reported to be important for podosome formation and function in osteoclasts. However, podosome-like structures have been reported in gelsolin-deficient dendritic cells, but the identity of these structures was not confirmed, and their dynamics and function was not investigated. Like many other cells, dendritic cells of the immune system also form matrix degrading podosomes. In the present study, we show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. Furthermore, we found that gelsolin is not required for TLR4-induced podosome disassembly. Thus, the actin cytoskeleton of podosomes involved in dendritic cell extracellular matrix degradation appears to be regulated differently than the cytoskeleton in podosomes of osteoclasts mediating bone resorption.Oscar HammarfjordHervé FaletChristine GurniakJohn H HartwigRobert P A WallinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21615 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin Gelsolin-independent podosome formation in dendritic cells.
description	Podosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filament scaffolds onto which proteins important for their function, such as matrix metallo-proteases and integrins, attach. The dynamics of the podosomes require the action of many proteins regulating actin assembly and disassembly. One such protein, gelsolin, which associates to podosomes, has been reported to be important for podosome formation and function in osteoclasts. However, podosome-like structures have been reported in gelsolin-deficient dendritic cells, but the identity of these structures was not confirmed, and their dynamics and function was not investigated. Like many other cells, dendritic cells of the immune system also form matrix degrading podosomes. In the present study, we show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. Furthermore, we found that gelsolin is not required for TLR4-induced podosome disassembly. Thus, the actin cytoskeleton of podosomes involved in dendritic cell extracellular matrix degradation appears to be regulated differently than the cytoskeleton in podosomes of osteoclasts mediating bone resorption.
format	article
author	Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin
author_facet	Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin
author_sort	Oscar Hammarfjord
title	Gelsolin-independent podosome formation in dendritic cells.
title_short	Gelsolin-independent podosome formation in dendritic cells.
title_full	Gelsolin-independent podosome formation in dendritic cells.
title_fullStr	Gelsolin-independent podosome formation in dendritic cells.
title_full_unstemmed	Gelsolin-independent podosome formation in dendritic cells.
title_sort	gelsolin-independent podosome formation in dendritic cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/990cd1672b7d4f18ab0170464c3c1b4b
work_keys_str_mv	AT oscarhammarfjord gelsolinindependentpodosomeformationindendriticcells AT hervefalet gelsolinindependentpodosomeformationindendriticcells AT christinegurniak gelsolinindependentpodosomeformationindendriticcells AT johnhhartwig gelsolinindependentpodosomeformationindendriticcells AT robertpawallin gelsolinindependentpodosomeformationindendriticcells
_version_	1718424308158562304

Gelsolin-independent podosome formation in dendritic cells.

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