Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.

Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in viv...

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Autores principales:	Hanneke van Zoggel, Gilles Carpentier, Célia Dos Santos, Yamina Hamma-Kourbali, José Courty, Mohamed Amiche, Jean Delbé
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9911662aeebc4a99ba9aa6590f8c9104
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spelling	oai:doaj.org-article:9911662aeebc4a99ba9aa6590f8c91042021-11-18T07:04:49ZAntitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.1932-620310.1371/journal.pone.0044351https://doaj.org/article/9911662aeebc4a99ba9aa6590f8c91042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028527/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in vivo antitumor activity of this molecule and investigated its mechanism of action. We showed that Drs B2 inhibits the proliferation and colony formation of various human tumor cell types, and the proliferation and capillary formation of endothelial cells in vitro. Furthermore, Drs B2 inhibited tumor growth of the human prostate adenocarcinoma cell line PC3 in a xenograft model in vivo. Research on the mechanism of action of Drs B2 on tumor PC3 cells demonstrated a rapid increasing amount of cytosolic lactate dehydrogenase, no activation of caspase-3, and no changes in mitochondrial membrane potential. Confocal microscopy analysis revealed that Drs B2 can interact with the tumor cell surface, aggregate and penetrate the cells. These data together indicate that Drs B2 does not act by apoptosis but possibly by necrosis. In conclusion, Drs B2 could be considered as an interesting and promising pharmacological and therapeutic leader molecule for the treatment of cancer.Hanneke van ZoggelGilles CarpentierCélia Dos SantosYamina Hamma-KourbaliJosé CourtyMohamed AmicheJean DelbéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44351 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanneke van Zoggel Gilles Carpentier Célia Dos Santos Yamina Hamma-Kourbali José Courty Mohamed Amiche Jean Delbé Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
description	Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in vivo antitumor activity of this molecule and investigated its mechanism of action. We showed that Drs B2 inhibits the proliferation and colony formation of various human tumor cell types, and the proliferation and capillary formation of endothelial cells in vitro. Furthermore, Drs B2 inhibited tumor growth of the human prostate adenocarcinoma cell line PC3 in a xenograft model in vivo. Research on the mechanism of action of Drs B2 on tumor PC3 cells demonstrated a rapid increasing amount of cytosolic lactate dehydrogenase, no activation of caspase-3, and no changes in mitochondrial membrane potential. Confocal microscopy analysis revealed that Drs B2 can interact with the tumor cell surface, aggregate and penetrate the cells. These data together indicate that Drs B2 does not act by apoptosis but possibly by necrosis. In conclusion, Drs B2 could be considered as an interesting and promising pharmacological and therapeutic leader molecule for the treatment of cancer.
format	article
author	Hanneke van Zoggel Gilles Carpentier Célia Dos Santos Yamina Hamma-Kourbali José Courty Mohamed Amiche Jean Delbé
author_facet	Hanneke van Zoggel Gilles Carpentier Célia Dos Santos Yamina Hamma-Kourbali José Courty Mohamed Amiche Jean Delbé
author_sort	Hanneke van Zoggel
title	Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
title_short	Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
title_full	Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
title_fullStr	Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
title_full_unstemmed	Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.
title_sort	antitumor and angiostatic activities of the antimicrobial peptide dermaseptin b2.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9911662aeebc4a99ba9aa6590f8c9104
work_keys_str_mv	AT hannekevanzoggel antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT gillescarpentier antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT celiadossantos antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT yaminahammakourbali antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT josecourty antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT mohamedamiche antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT jeandelbe antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2
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Antitumor and angiostatic activities of the antimicrobial peptide dermaseptin B2.

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