Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats

Xing-Jie Li,1,* Li Liang,2,* Hong-Xia Shi,1 Xiao-Ping Sun,1 Jing Wang,1 Lian-Sheng Zhang1 1Health Management Center, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China; 2Department of Pharmacy, Lanzhou University Second Hospital, Lanzhou, People’s Republic o...

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Autores principales:	Li XJ, Liang L, Shi HX, Sun XP, Wang J, Zhang LS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Curdione cerebral ischemia reperfusion oxidative stress apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/99147b2674a7456987dc766b8d54ff77
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spelling	oai:doaj.org-article:99147b2674a7456987dc766b8d54ff772021-12-02T01:26:04ZNeuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats1178-2021https://doaj.org/article/99147b2674a7456987dc766b8d54ff772017-06-01T00:00:00Zhttps://www.dovepress.com/neuroprotective-effects-of-curdione-against-focal-cerebral-ischemia-re-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Xing-Jie Li,1,* Li Liang,2,* Hong-Xia Shi,1 Xiao-Ping Sun,1 Jing Wang,1 Lian-Sheng Zhang1 1Health Management Center, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China; 2Department of Pharmacy, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China *These authors contributed equally to this work Background: Curdione is one of the most highly concentrated component of the active constituents in E-zhu, which has been reported to possess a variety of activities. However, the pharmacologic neuroprotective activity of curdione has not been evaluated. The present study aimed to investigate the protective effect of curdione on focal cerebral ischemia reperfusion-induced injury in rats and further exploring the underlying mechanisms. Materials and methods: Adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO) surgery for 2 h, followed by reperfusion stage. All animals received treatment once a day for 7 days before surgery and 14 days from 4 h after the reperfusion started. The neurological deficit test and Morris water maze test were performed at 1, 4, 7 and 14 days after MCAO. The infarct size of animals was determined by the 2,3,5-triphenyltetrazolium chloride staining, and pathological brain damage was estimated by hematoxylin–eosin staining. The malonaldehyde (MDA) levels and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) were detected by enzyme-linked immunosorbent assay. Expression of apoptotic proteins was measured by Western blot. Results: Our results showed that curdione could significantly reduce the infarct size and neurological deficits, promote cognitive function recovery and recover neuronal morphologic damages in MCAO rats. It also blocked the increase of MDA content and elevated the activities of SOD, CAT and GSH-PX. Moreover, curdione attenuated the expression of Cyt-C, c-caspase-3 and c-caspase-9 increased the Bcl-2/Bax ratio and hence decreased the cellular apoptosis. Conclusion: Curdione possessed potential neuroprotective effect on rats in the MCAO model. The anti-oxidative and anti-apoptotic properties may be involved in the underlying mechanisms. Keywords: curdione, cerebral ischemia reperfusion, oxidative stress, apoptosis Li XJLiang LShi HXSun XPWang JZhang LSDove Medical PressarticleCurdionecerebral ischemia reperfusionoxidative stressapoptosisNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 13, Pp 1733-1740 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Curdione cerebral ischemia reperfusion oxidative stress apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Curdione cerebral ischemia reperfusion oxidative stress apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Li XJ Liang L Shi HX Sun XP Wang J Zhang LS Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
description	Xing-Jie Li,1,* Li Liang,2,* Hong-Xia Shi,1 Xiao-Ping Sun,1 Jing Wang,1 Lian-Sheng Zhang1 1Health Management Center, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China; 2Department of Pharmacy, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China *These authors contributed equally to this work Background: Curdione is one of the most highly concentrated component of the active constituents in E-zhu, which has been reported to possess a variety of activities. However, the pharmacologic neuroprotective activity of curdione has not been evaluated. The present study aimed to investigate the protective effect of curdione on focal cerebral ischemia reperfusion-induced injury in rats and further exploring the underlying mechanisms. Materials and methods: Adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO) surgery for 2 h, followed by reperfusion stage. All animals received treatment once a day for 7 days before surgery and 14 days from 4 h after the reperfusion started. The neurological deficit test and Morris water maze test were performed at 1, 4, 7 and 14 days after MCAO. The infarct size of animals was determined by the 2,3,5-triphenyltetrazolium chloride staining, and pathological brain damage was estimated by hematoxylin–eosin staining. The malonaldehyde (MDA) levels and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) were detected by enzyme-linked immunosorbent assay. Expression of apoptotic proteins was measured by Western blot. Results: Our results showed that curdione could significantly reduce the infarct size and neurological deficits, promote cognitive function recovery and recover neuronal morphologic damages in MCAO rats. It also blocked the increase of MDA content and elevated the activities of SOD, CAT and GSH-PX. Moreover, curdione attenuated the expression of Cyt-C, c-caspase-3 and c-caspase-9 increased the Bcl-2/Bax ratio and hence decreased the cellular apoptosis. Conclusion: Curdione possessed potential neuroprotective effect on rats in the MCAO model. The anti-oxidative and anti-apoptotic properties may be involved in the underlying mechanisms. Keywords: curdione, cerebral ischemia reperfusion, oxidative stress, apoptosis
format	article
author	Li XJ Liang L Shi HX Sun XP Wang J Zhang LS
author_facet	Li XJ Liang L Shi HX Sun XP Wang J Zhang LS
author_sort	Li XJ
title	Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
title_short	Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
title_full	Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
title_fullStr	Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
title_full_unstemmed	Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
title_sort	neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/99147b2674a7456987dc766b8d54ff77
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Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats

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