Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii

Abstract Hyriopsis cumingii is an important species for freshwater pearl cultivation in China. In terms of pearl production, males have larger pearls and better glossiness than females, but there are few reports focusing on the sex of H. cumingii. In this study, six mRNA and six microRNA (miRNA) lib...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ya-Yu Wang, Sheng-Hua Duan, Gui-Ling Wang, Jia-Le Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9916add4c1cd4afda7fede2108927b94
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9916add4c1cd4afda7fede2108927b94
record_format	dspace
spelling	oai:doaj.org-article:9916add4c1cd4afda7fede2108927b942021-12-02T14:01:20ZIntegrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii10.1038/s41598-020-80264-72045-2322https://doaj.org/article/9916add4c1cd4afda7fede2108927b942021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80264-7https://doaj.org/toc/2045-2322Abstract Hyriopsis cumingii is an important species for freshwater pearl cultivation in China. In terms of pearl production, males have larger pearls and better glossiness than females, but there are few reports focusing on the sex of H. cumingii. In this study, six mRNA and six microRNA (miRNA) libraries were prepared from ovaries and testes. Additionally, 28,502 differentially expressed genes (DEGs) and 32 differentially expressed miRNAs (DEMs) were identified. Compared with testis, 14,360 mRNAs and 20 miRNAs were up-regulated in ovary, 14,142 mRNAs and 12 miRNAs were down-regulated. In DEGs, the known genes related to sex determinism and/or differentiation were also identified, such as DMRT1, SOX9, SF1 for males, FOXL2 for females, and other potentially significant candidate genes. Three sex-related pathways have also been identified, which are Wnt, Notch, and TGF-beta. In 32 DEMs, the three miRNAs (miR-9-5p, miR-92, miR-184) were paid more attention, they predicted 28 target genes, which may also be candidates for sex-related miRNAs and genes. Differential miRNAs target genes analysis reveals the pathway associated with oocyte meiosis and spermatogenesis. Overall, the findings of the study provide significant insights to enhance our understanding of sex differentiation and/or sex determination mechanisms for H. cumingii.Ya-Yu WangSheng-Hua DuanGui-Ling WangJia-Le LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ya-Yu Wang Sheng-Hua Duan Gui-Ling Wang Jia-Le Li Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
description	Abstract Hyriopsis cumingii is an important species for freshwater pearl cultivation in China. In terms of pearl production, males have larger pearls and better glossiness than females, but there are few reports focusing on the sex of H. cumingii. In this study, six mRNA and six microRNA (miRNA) libraries were prepared from ovaries and testes. Additionally, 28,502 differentially expressed genes (DEGs) and 32 differentially expressed miRNAs (DEMs) were identified. Compared with testis, 14,360 mRNAs and 20 miRNAs were up-regulated in ovary, 14,142 mRNAs and 12 miRNAs were down-regulated. In DEGs, the known genes related to sex determinism and/or differentiation were also identified, such as DMRT1, SOX9, SF1 for males, FOXL2 for females, and other potentially significant candidate genes. Three sex-related pathways have also been identified, which are Wnt, Notch, and TGF-beta. In 32 DEMs, the three miRNAs (miR-9-5p, miR-92, miR-184) were paid more attention, they predicted 28 target genes, which may also be candidates for sex-related miRNAs and genes. Differential miRNAs target genes analysis reveals the pathway associated with oocyte meiosis and spermatogenesis. Overall, the findings of the study provide significant insights to enhance our understanding of sex differentiation and/or sex determination mechanisms for H. cumingii.
format	article
author	Ya-Yu Wang Sheng-Hua Duan Gui-Ling Wang Jia-Le Li
author_facet	Ya-Yu Wang Sheng-Hua Duan Gui-Ling Wang Jia-Le Li
author_sort	Ya-Yu Wang
title	Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
title_short	Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
title_full	Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
title_fullStr	Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
title_full_unstemmed	Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii
title_sort	integrated mrna and mirna expression profile analysis of female and male gonads in hyriopsis cumingii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9916add4c1cd4afda7fede2108927b94
work_keys_str_mv	AT yayuwang integratedmrnaandmirnaexpressionprofileanalysisoffemaleandmalegonadsinhyriopsiscumingii AT shenghuaduan integratedmrnaandmirnaexpressionprofileanalysisoffemaleandmalegonadsinhyriopsiscumingii AT guilingwang integratedmrnaandmirnaexpressionprofileanalysisoffemaleandmalegonadsinhyriopsiscumingii AT jialeli integratedmrnaandmirnaexpressionprofileanalysisoffemaleandmalegonadsinhyriopsiscumingii
_version_	1718392215526440960

Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii

Ejemplares similares