Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.

<h4>Objective</h4>To evaluate the in situ antibacterial activity of a mouthrinse with 0.2% Chlorhexidine (M-0.2% CHX) on undisturbed de novo plaque-like biofilm (PL-biofilm) and on salivary flora up to 7 hours after its application.<h4>Methods</h4>A special acrylic appliance...

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Autores principales:	Lucía García-Caballero, Victor Quintas, Isabel Prada-López, Juan Seoane, Nikos Donos, Inmaculada Tomás
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9919c35f8f9f4e7f960ef417b951b219
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spelling	oai:doaj.org-article:9919c35f8f9f4e7f960ef417b951b2192021-11-18T08:40:09ZChlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.1932-620310.1371/journal.pone.0083522https://doaj.org/article/9919c35f8f9f4e7f960ef417b951b2192013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386220/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To evaluate the in situ antibacterial activity of a mouthrinse with 0.2% Chlorhexidine (M-0.2% CHX) on undisturbed de novo plaque-like biofilm (PL-biofilm) and on salivary flora up to 7 hours after its application.<h4>Methods</h4>A special acrylic appliance was designed, with 3 inserted glass disks on each buccal side, allowing for PL-biofilm growth. Fifteen healthy volunteers wore the appliance for 48 hours and then performed an M-0.2% CHX; disks were removed at 30 seconds and 1, 3, 5 and 7 hours after the mouth-rinsing. Applying a washout period, saliva samples were collected from each volunteer at 30 seconds and 1, 3, 5 and 7 hours after performing an M-0.2% CHX. The PL-biofilm and saliva samples were analysed by confocal laser scanning and epifluorescence microscopes, respectively.<h4>Results</h4>At 30 seconds after M-0.2% CHX, the levels of viable bacteria detected in saliva were significantly lower than those observed in PL-biofilm. The difference in the percentage of live bacteria detected in saliva was significantly higher than that observed in PL-biofilm at 5 and 7 hours after M-0.2% CHX.<h4>Conclusion</h4>After a single mouthrinse of the 0.2% CHX formulation tested in the present study, the 2-day PL-biofilm presented a significantly higher resistance to this antiseptic in situ than that observed in salivary flora. However, this 0.2% CHX formulation showed a higher substantivity on PL-biofilm than on salivary flora at 5 and 7 hours after mouth-rinsing, which could be related to the slower growth rate of PL-biofilm and the possible reservoir function for antimicrobial agents associated with the undisturbed de novo PL-biofilm.Lucía García-CaballeroVictor QuintasIsabel Prada-LópezJuan SeoaneNikos DonosInmaculada TomásPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83522 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucía García-Caballero Victor Quintas Isabel Prada-López Juan Seoane Nikos Donos Inmaculada Tomás Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
description	<h4>Objective</h4>To evaluate the in situ antibacterial activity of a mouthrinse with 0.2% Chlorhexidine (M-0.2% CHX) on undisturbed de novo plaque-like biofilm (PL-biofilm) and on salivary flora up to 7 hours after its application.<h4>Methods</h4>A special acrylic appliance was designed, with 3 inserted glass disks on each buccal side, allowing for PL-biofilm growth. Fifteen healthy volunteers wore the appliance for 48 hours and then performed an M-0.2% CHX; disks were removed at 30 seconds and 1, 3, 5 and 7 hours after the mouth-rinsing. Applying a washout period, saliva samples were collected from each volunteer at 30 seconds and 1, 3, 5 and 7 hours after performing an M-0.2% CHX. The PL-biofilm and saliva samples were analysed by confocal laser scanning and epifluorescence microscopes, respectively.<h4>Results</h4>At 30 seconds after M-0.2% CHX, the levels of viable bacteria detected in saliva were significantly lower than those observed in PL-biofilm. The difference in the percentage of live bacteria detected in saliva was significantly higher than that observed in PL-biofilm at 5 and 7 hours after M-0.2% CHX.<h4>Conclusion</h4>After a single mouthrinse of the 0.2% CHX formulation tested in the present study, the 2-day PL-biofilm presented a significantly higher resistance to this antiseptic in situ than that observed in salivary flora. However, this 0.2% CHX formulation showed a higher substantivity on PL-biofilm than on salivary flora at 5 and 7 hours after mouth-rinsing, which could be related to the slower growth rate of PL-biofilm and the possible reservoir function for antimicrobial agents associated with the undisturbed de novo PL-biofilm.
format	article
author	Lucía García-Caballero Victor Quintas Isabel Prada-López Juan Seoane Nikos Donos Inmaculada Tomás
author_facet	Lucía García-Caballero Victor Quintas Isabel Prada-López Juan Seoane Nikos Donos Inmaculada Tomás
author_sort	Lucía García-Caballero
title	Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
title_short	Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
title_full	Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
title_fullStr	Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
title_full_unstemmed	Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
title_sort	chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9919c35f8f9f4e7f960ef417b951b219
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Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model.

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