MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1

Abstract Background This study was designed to investigate the mechanism and effects of miRNA-221-5p on the T-helper 17 (Th17)/T-regulatory (Treg) ratio in asthma. Methods BALB/c mice were intranasally challenged with 100 µg OVA on 14 and 21 day. Mice were rechallenged with 2.5% OVA-PBS on 22 and 28...

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Autores principales:	Yuanyuan Guan, Yuemei Ma, Yao Tang, Xiaolei Liu, Yan Zhao, Lixin An
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Asthma miRNA-221-5p RORγt SOCS1 Th17/Treg Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/991c18e374b7471587e245d0a7bbe662
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spelling	oai:doaj.org-article:991c18e374b7471587e245d0a7bbe6622021-12-05T12:23:20ZMiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS110.1186/s13223-021-00620-81710-1492https://doaj.org/article/991c18e374b7471587e245d0a7bbe6622021-12-01T00:00:00Zhttps://doi.org/10.1186/s13223-021-00620-8https://doaj.org/toc/1710-1492Abstract Background This study was designed to investigate the mechanism and effects of miRNA-221-5p on the T-helper 17 (Th17)/T-regulatory (Treg) ratio in asthma. Methods BALB/c mice were intranasally challenged with 100 µg OVA on 14 and 21 day. Mice were rechallenged with 2.5% OVA-PBS on 22 and 28 day. Mice were sacrificed using on day 30 under 35 mg/kg pentobarbital sodium. PBMCs were induced vitro model of asthma using 500 ng of lipopolysaccharides (LPS) for 4 h. Results The expression of miRNA-221-5p was reduced in in vivo model, compared sham group. The vitro model of asthma treated with miRNA-221-5p mimic resulted in the reduction of IL-6, IL-17, IL-21 and IL-22 levels, and induction of IL-10, IL-35 and TGF-β levels. In addition, down-regulation of miRNA-221-5p induced the protein expression of suppressor of cytokine signaling 1 (SOCS1) and receptor-related orphan receptor-gamma-t (RORγt) and suppressed that of FOXP3 in in vitro model of asthma. Over-expression of miRNA-221-5p induced the protein expression of FOXP3, and suppressed that of SOCS1 and RORγt in in vitro model of asthma. The inhibition of SOCS1 or RORγt attenuated the effects of anti-miRNA-221-5p on Th17/Treg ratio in asthma. Conclusion miRNA-221-5p may play critical roles in driving the differentiation of Th17/Treg ratio via RORγt/Foxp3 by Targeting SOCS1, reduced the function of Th17 cells by directly inhibiting RORγt/SOCS1 and promoted the function of Treg cells via Foxp3/ SOCS1 in asthma.Yuanyuan GuanYuemei MaYao TangXiaolei LiuYan ZhaoLixin AnBMCarticleAsthmamiRNA-221-5pRORγtSOCS1Th17/TregImmunologic diseases. AllergyRC581-607ENAllergy, Asthma & Clinical Immunology, Vol 17, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Asthma miRNA-221-5p RORγt SOCS1 Th17/Treg Immunologic diseases. Allergy RC581-607
spellingShingle	Asthma miRNA-221-5p RORγt SOCS1 Th17/Treg Immunologic diseases. Allergy RC581-607 Yuanyuan Guan Yuemei Ma Yao Tang Xiaolei Liu Yan Zhao Lixin An MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
description	Abstract Background This study was designed to investigate the mechanism and effects of miRNA-221-5p on the T-helper 17 (Th17)/T-regulatory (Treg) ratio in asthma. Methods BALB/c mice were intranasally challenged with 100 µg OVA on 14 and 21 day. Mice were rechallenged with 2.5% OVA-PBS on 22 and 28 day. Mice were sacrificed using on day 30 under 35 mg/kg pentobarbital sodium. PBMCs were induced vitro model of asthma using 500 ng of lipopolysaccharides (LPS) for 4 h. Results The expression of miRNA-221-5p was reduced in in vivo model, compared sham group. The vitro model of asthma treated with miRNA-221-5p mimic resulted in the reduction of IL-6, IL-17, IL-21 and IL-22 levels, and induction of IL-10, IL-35 and TGF-β levels. In addition, down-regulation of miRNA-221-5p induced the protein expression of suppressor of cytokine signaling 1 (SOCS1) and receptor-related orphan receptor-gamma-t (RORγt) and suppressed that of FOXP3 in in vitro model of asthma. Over-expression of miRNA-221-5p induced the protein expression of FOXP3, and suppressed that of SOCS1 and RORγt in in vitro model of asthma. The inhibition of SOCS1 or RORγt attenuated the effects of anti-miRNA-221-5p on Th17/Treg ratio in asthma. Conclusion miRNA-221-5p may play critical roles in driving the differentiation of Th17/Treg ratio via RORγt/Foxp3 by Targeting SOCS1, reduced the function of Th17 cells by directly inhibiting RORγt/SOCS1 and promoted the function of Treg cells via Foxp3/ SOCS1 in asthma.
format	article
author	Yuanyuan Guan Yuemei Ma Yao Tang Xiaolei Liu Yan Zhao Lixin An
author_facet	Yuanyuan Guan Yuemei Ma Yao Tang Xiaolei Liu Yan Zhao Lixin An
author_sort	Yuanyuan Guan
title	MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
title_short	MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
title_full	MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
title_fullStr	MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
title_full_unstemmed	MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
title_sort	mirna-221-5p suppressed the th17/treg ratio in asthma via rorγt/foxp3 by targeting socs1
publisher	BMC
publishDate	2021
url	https://doaj.org/article/991c18e374b7471587e245d0a7bbe662
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MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1

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