Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus

Abstract. Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of...

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Autores principales:	Muhammad Evy Prastiyanto, RIZAL MAARIF RUKMANA, DEWI KUNTHY SARASWATI, SRI DARMAWATI, ENDANG TRI WAHYUNI MAHARANI, YANUARITA TURSINAWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antibacterial activity, anticancer, mrsa, mushrooms, pleurotus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/991c2e314a78481ab1ef01a26260bc94
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spelling	oai:doaj.org-article:991c2e314a78481ab1ef01a26260bc942021-11-22T00:52:01ZAnticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus1412-033X2085-472210.13057/biodiv/d211221https://doaj.org/article/991c2e314a78481ab1ef01a26260bc942020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6957https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of this work is to identify the potential effects of methanolic extracts from four species of the Pleurotus genus cultivated in Indonesia on nasopharynx cancer (Raji cell line), and to investigate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). This study investigates four species members of Pleurotus (Pleurotus ostreatus, P. cystidiosus, P. flabellatus, and P. pulmonarius var. stechangii). Dry Samples were extracted with methanol to yield crude extracts. Cytotoxicity screening was conducted using MTT assay of dry extracts, while antibacterial activity was calculated based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Mueller–Hinton broth, via the microdilution method. Compounds were analyzed using thin-layer chromatography (TLC). P. flabellatus provided the highest yield of dry extract (20.8%) with the lowest value of IC50 (556.226 µg/mL) compared to the three other species investigated. Antibacterial activity was calculated as MIC and MBC values against MRSA by the P. flabellatus extract which reached 6.25 mg/mL and 250 mg/mL, respectively. The result of TLC of the dry extract of P. flabellatus revealed the presence of terpenoids. P. flabellatus has the potential to be developed as both an anti-cancer and an antibacterial agent, especially against Raji cells and MRSA strains. However, further in vivo research and discovery of the modes of action involved are still needed to shed light on these effects. Studies can provide new information about the benefits of Pleurotus as a source of natural anticancer and antibacterial compound.Muhammad Evy PrastiyantoRIZAL MAARIF RUKMANADEWI KUNTHY SARASWATISRI DARMAWATIENDANG TRI WAHYUNI MAHARANIYANUARITA TURSINAWATIMBI & UNS Soloarticleantibacterial activity, anticancer, mrsa, mushrooms, pleurotusBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial activity, anticancer, mrsa, mushrooms, pleurotus Biology (General) QH301-705.5
spellingShingle	antibacterial activity, anticancer, mrsa, mushrooms, pleurotus Biology (General) QH301-705.5 Muhammad Evy Prastiyanto RIZAL MAARIF RUKMANA DEWI KUNTHY SARASWATI SRI DARMAWATI ENDANG TRI WAHYUNI MAHARANI YANUARITA TURSINAWATI Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
description	Abstract. Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of this work is to identify the potential effects of methanolic extracts from four species of the Pleurotus genus cultivated in Indonesia on nasopharynx cancer (Raji cell line), and to investigate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). This study investigates four species members of Pleurotus (Pleurotus ostreatus, P. cystidiosus, P. flabellatus, and P. pulmonarius var. stechangii). Dry Samples were extracted with methanol to yield crude extracts. Cytotoxicity screening was conducted using MTT assay of dry extracts, while antibacterial activity was calculated based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Mueller–Hinton broth, via the microdilution method. Compounds were analyzed using thin-layer chromatography (TLC). P. flabellatus provided the highest yield of dry extract (20.8%) with the lowest value of IC50 (556.226 µg/mL) compared to the three other species investigated. Antibacterial activity was calculated as MIC and MBC values against MRSA by the P. flabellatus extract which reached 6.25 mg/mL and 250 mg/mL, respectively. The result of TLC of the dry extract of P. flabellatus revealed the presence of terpenoids. P. flabellatus has the potential to be developed as both an anti-cancer and an antibacterial agent, especially against Raji cells and MRSA strains. However, further in vivo research and discovery of the modes of action involved are still needed to shed light on these effects. Studies can provide new information about the benefits of Pleurotus as a source of natural anticancer and antibacterial compound.
format	article
author	Muhammad Evy Prastiyanto RIZAL MAARIF RUKMANA DEWI KUNTHY SARASWATI SRI DARMAWATI ENDANG TRI WAHYUNI MAHARANI YANUARITA TURSINAWATI
author_facet	Muhammad Evy Prastiyanto RIZAL MAARIF RUKMANA DEWI KUNTHY SARASWATI SRI DARMAWATI ENDANG TRI WAHYUNI MAHARANI YANUARITA TURSINAWATI
author_sort	Muhammad Evy Prastiyanto
title	Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
title_short	Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
title_full	Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
title_fullStr	Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed	Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
title_sort	anticancer potential of methanolic extracts from pleurotus species on raji cells and antibacterial activity against methicillin-resistant staphylococcus aureus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/991c2e314a78481ab1ef01a26260bc94
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Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus

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