Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.

Amphiphilic block co-polymer nanoparticles are interesting candidates for drug delivery as a result of their unique properties such as the size, modularity, biocompatibility and drug loading capacity. They can be rapidly formulated in a nanoprecipitation process based on self-assembly, resulting in...

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Autores principales:	Adrianna Glinkowska Mares, Gaia Pacassoni, Josep Samitier Marti, Silvia Pujals, Lorenzo Albertazzi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/991caf3405aa4f0e86a81885e23d038d
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spelling	oai:doaj.org-article:991caf3405aa4f0e86a81885e23d038d2021-12-02T20:05:19ZFormulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.1932-620310.1371/journal.pone.0251821https://doaj.org/article/991caf3405aa4f0e86a81885e23d038d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251821https://doaj.org/toc/1932-6203Amphiphilic block co-polymer nanoparticles are interesting candidates for drug delivery as a result of their unique properties such as the size, modularity, biocompatibility and drug loading capacity. They can be rapidly formulated in a nanoprecipitation process based on self-assembly, resulting in kinetically locked nanostructures. The control over this step allows us to obtain nanoparticles with tailor-made properties without modification of the co-polymer building blocks. Furthermore, a reproducible and controlled formulation supports better predictability of a batch effectiveness in preclinical tests. Herein, we compared the formulation of PLGA-PEG nanoparticles using the typical manual bulk mixing and a microfluidic chip-assisted nanoprecipitation. The particle size tunability and controllability in a hydrodynamic flow focusing device was demonstrated to be greater than in the manual dropwise addition method. We also analyzed particle size and encapsulation of fluorescent compounds, using the common bulk analysis and advanced microscopy techniques: Transmission Electron Microscopy and Total Internal Reflection Microscopy, to reveal the heterogeneities occurred in the formulated nanoparticles. Finally, we performed in vitro evaluation of obtained NPs using MCF-7 cell line. Our results show how the microfluidic formulation improves the fine control over the resulting nanoparticles, without compromising any appealing property of PLGA nanoparticle. The combination of microfluidic formulation with advanced analysis methods, looking at the single particle level, can improve the understanding of the NP properties, heterogeneities and performance.Adrianna Glinkowska MaresGaia PacassoniJosep Samitier MartiSilvia PujalsLorenzo AlbertazziPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251821 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrianna Glinkowska Mares Gaia Pacassoni Josep Samitier Marti Silvia Pujals Lorenzo Albertazzi Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
description	Amphiphilic block co-polymer nanoparticles are interesting candidates for drug delivery as a result of their unique properties such as the size, modularity, biocompatibility and drug loading capacity. They can be rapidly formulated in a nanoprecipitation process based on self-assembly, resulting in kinetically locked nanostructures. The control over this step allows us to obtain nanoparticles with tailor-made properties without modification of the co-polymer building blocks. Furthermore, a reproducible and controlled formulation supports better predictability of a batch effectiveness in preclinical tests. Herein, we compared the formulation of PLGA-PEG nanoparticles using the typical manual bulk mixing and a microfluidic chip-assisted nanoprecipitation. The particle size tunability and controllability in a hydrodynamic flow focusing device was demonstrated to be greater than in the manual dropwise addition method. We also analyzed particle size and encapsulation of fluorescent compounds, using the common bulk analysis and advanced microscopy techniques: Transmission Electron Microscopy and Total Internal Reflection Microscopy, to reveal the heterogeneities occurred in the formulated nanoparticles. Finally, we performed in vitro evaluation of obtained NPs using MCF-7 cell line. Our results show how the microfluidic formulation improves the fine control over the resulting nanoparticles, without compromising any appealing property of PLGA nanoparticle. The combination of microfluidic formulation with advanced analysis methods, looking at the single particle level, can improve the understanding of the NP properties, heterogeneities and performance.
format	article
author	Adrianna Glinkowska Mares Gaia Pacassoni Josep Samitier Marti Silvia Pujals Lorenzo Albertazzi
author_facet	Adrianna Glinkowska Mares Gaia Pacassoni Josep Samitier Marti Silvia Pujals Lorenzo Albertazzi
author_sort	Adrianna Glinkowska Mares
title	Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
title_short	Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
title_full	Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
title_fullStr	Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
title_full_unstemmed	Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.
title_sort	formulation of tunable size plga-peg nanoparticles for drug delivery using microfluidic technology.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/991caf3405aa4f0e86a81885e23d038d
work_keys_str_mv	AT adriannaglinkowskamares formulationoftunablesizeplgapegnanoparticlesfordrugdeliveryusingmicrofluidictechnology AT gaiapacassoni formulationoftunablesizeplgapegnanoparticlesfordrugdeliveryusingmicrofluidictechnology AT josepsamitiermarti formulationoftunablesizeplgapegnanoparticlesfordrugdeliveryusingmicrofluidictechnology AT silviapujals formulationoftunablesizeplgapegnanoparticlesfordrugdeliveryusingmicrofluidictechnology AT lorenzoalbertazzi formulationoftunablesizeplgapegnanoparticlesfordrugdeliveryusingmicrofluidictechnology
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Formulation of tunable size PLGA-PEG nanoparticles for drug delivery using microfluidic technology.

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