Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.

Staphylococcus aureus RNAIII is the intracellular effector of the quorum sensing system that temporally controls a large number of virulence factors including exoproteins and cell-wall-associated proteins. Staphylocoagulase is one major virulence factor, which promotes clotting of human plasma. Like...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Clément Chevalier, Sandrine Boisset, Cédric Romilly, Benoit Masquida, Pierre Fechter, Thomas Geissmann, François Vandenesch, Pascale Romby
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/991d745a3ba84f7eb3eccac0f951b25f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:991d745a3ba84f7eb3eccac0f951b25f
record_format	dspace
spelling	oai:doaj.org-article:991d745a3ba84f7eb3eccac0f951b25f2021-11-25T05:48:14ZStaphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.1553-73661553-737410.1371/journal.ppat.1000809https://doaj.org/article/991d745a3ba84f7eb3eccac0f951b25f2010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20300607/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Staphylococcus aureus RNAIII is the intracellular effector of the quorum sensing system that temporally controls a large number of virulence factors including exoproteins and cell-wall-associated proteins. Staphylocoagulase is one major virulence factor, which promotes clotting of human plasma. Like the major cell surface protein A, the expression of staphylocoagulase is strongly repressed by the quorum sensing system at the post-exponential growth phase. Here we used a combination of approaches in vivo and in vitro to analyze the mechanism used by RNAIII to regulate the expression of staphylocoagulase. Our data show that RNAIII represses the synthesis of the protein through a direct binding with the mRNA. Structure mapping shows that two distant regions of RNAIII interact with coa mRNA and that the mRNA harbors a conserved signature as found in other RNAIII-target mRNAs. The resulting complex is composed of an imperfect duplex masking the Shine-Dalgarno sequence of coa mRNA and of a loop-loop interaction occurring downstream in the coding region. The imperfect duplex is sufficient to prevent the formation of the ribosomal initiation complex and to repress the expression of a reporter gene in vivo. In addition, the double-strand-specific endoribonuclease III cleaves the two regions of the mRNA bound to RNAIII that may contribute to the degradation of the repressed mRNA. This study validates another direct target of RNAIII that plays a role in virulence. It also illustrates the diversity of RNAIII-mRNA topologies and how these multiple RNAIII-mRNA interactions would mediate virulence regulation.Clément ChevalierSandrine BoissetCédric RomillyBenoit MasquidaPierre FechterThomas GeissmannFrançois VandeneschPascale RombyPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 3, p e1000809 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Clément Chevalier Sandrine Boisset Cédric Romilly Benoit Masquida Pierre Fechter Thomas Geissmann François Vandenesch Pascale Romby Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
description	Staphylococcus aureus RNAIII is the intracellular effector of the quorum sensing system that temporally controls a large number of virulence factors including exoproteins and cell-wall-associated proteins. Staphylocoagulase is one major virulence factor, which promotes clotting of human plasma. Like the major cell surface protein A, the expression of staphylocoagulase is strongly repressed by the quorum sensing system at the post-exponential growth phase. Here we used a combination of approaches in vivo and in vitro to analyze the mechanism used by RNAIII to regulate the expression of staphylocoagulase. Our data show that RNAIII represses the synthesis of the protein through a direct binding with the mRNA. Structure mapping shows that two distant regions of RNAIII interact with coa mRNA and that the mRNA harbors a conserved signature as found in other RNAIII-target mRNAs. The resulting complex is composed of an imperfect duplex masking the Shine-Dalgarno sequence of coa mRNA and of a loop-loop interaction occurring downstream in the coding region. The imperfect duplex is sufficient to prevent the formation of the ribosomal initiation complex and to repress the expression of a reporter gene in vivo. In addition, the double-strand-specific endoribonuclease III cleaves the two regions of the mRNA bound to RNAIII that may contribute to the degradation of the repressed mRNA. This study validates another direct target of RNAIII that plays a role in virulence. It also illustrates the diversity of RNAIII-mRNA topologies and how these multiple RNAIII-mRNA interactions would mediate virulence regulation.
format	article
author	Clément Chevalier Sandrine Boisset Cédric Romilly Benoit Masquida Pierre Fechter Thomas Geissmann François Vandenesch Pascale Romby
author_facet	Clément Chevalier Sandrine Boisset Cédric Romilly Benoit Masquida Pierre Fechter Thomas Geissmann François Vandenesch Pascale Romby
author_sort	Clément Chevalier
title	Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
title_short	Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
title_full	Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
title_fullStr	Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
title_full_unstemmed	Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.
title_sort	staphylococcus aureus rnaiii binds to two distant regions of coa mrna to arrest translation and promote mrna degradation.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/991d745a3ba84f7eb3eccac0f951b25f
work_keys_str_mv	AT clementchevalier staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT sandrineboisset staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT cedricromilly staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT benoitmasquida staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT pierrefechter staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT thomasgeissmann staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT francoisvandenesch staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation AT pascaleromby staphylococcusaureusrnaiiibindstotwodistantregionsofcoamrnatoarresttranslationandpromotemrnadegradation
_version_	1718414472549236736

Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation.

Ejemplares similares