Fusion of protein aggregates facilitates asymmetric damage segregation.

Asymmetric segregation of damaged proteins at cell division generates a cell that retains damage and a clean cell that supports population survival. In cells that divide asymmetrically, such as Saccharomyces cerevisiae, segregation of damaged proteins is achieved by retention and active transport. W...

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Autores principales:	Miguel Coelho, Steven J Lade, Simon Alberti, Thilo Gross, Iva M Tolić
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/991e0c3fd5474dab9fbd13522abee3be
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spelling	oai:doaj.org-article:991e0c3fd5474dab9fbd13522abee3be2021-11-11T05:37:08ZFusion of protein aggregates facilitates asymmetric damage segregation.1544-91731545-788510.1371/journal.pbio.1001886https://doaj.org/article/991e0c3fd5474dab9fbd13522abee3be2014-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24936793/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Asymmetric segregation of damaged proteins at cell division generates a cell that retains damage and a clean cell that supports population survival. In cells that divide asymmetrically, such as Saccharomyces cerevisiae, segregation of damaged proteins is achieved by retention and active transport. We have previously shown that in the symmetrically dividing Schizosaccharomyces pombe there is a transition between symmetric and asymmetric segregation of damaged proteins. Yet how this transition and generation of damage-free cells are achieved remained unknown. Here, by combining in vivo imaging of Hsp104-associated aggregates, a form of damage, with mathematical modeling, we find that fusion of protein aggregates facilitates asymmetric segregation. Our model predicts that, after stress, the increased number of aggregates fuse into a single large unit, which is inherited asymmetrically by one daughter cell, whereas the other one is born clean. We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor. Our work shows that fusion of protein aggregates promotes the formation of damage-free cells. Fusion of cellular factors may represent a general mechanism for their asymmetric segregation at division.Miguel CoelhoSteven J LadeSimon AlbertiThilo GrossIva M TolićPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 6, p e1001886 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Miguel Coelho Steven J Lade Simon Alberti Thilo Gross Iva M Tolić Fusion of protein aggregates facilitates asymmetric damage segregation.
description	Asymmetric segregation of damaged proteins at cell division generates a cell that retains damage and a clean cell that supports population survival. In cells that divide asymmetrically, such as Saccharomyces cerevisiae, segregation of damaged proteins is achieved by retention and active transport. We have previously shown that in the symmetrically dividing Schizosaccharomyces pombe there is a transition between symmetric and asymmetric segregation of damaged proteins. Yet how this transition and generation of damage-free cells are achieved remained unknown. Here, by combining in vivo imaging of Hsp104-associated aggregates, a form of damage, with mathematical modeling, we find that fusion of protein aggregates facilitates asymmetric segregation. Our model predicts that, after stress, the increased number of aggregates fuse into a single large unit, which is inherited asymmetrically by one daughter cell, whereas the other one is born clean. We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor. Our work shows that fusion of protein aggregates promotes the formation of damage-free cells. Fusion of cellular factors may represent a general mechanism for their asymmetric segregation at division.
format	article
author	Miguel Coelho Steven J Lade Simon Alberti Thilo Gross Iva M Tolić
author_facet	Miguel Coelho Steven J Lade Simon Alberti Thilo Gross Iva M Tolić
author_sort	Miguel Coelho
title	Fusion of protein aggregates facilitates asymmetric damage segregation.
title_short	Fusion of protein aggregates facilitates asymmetric damage segregation.
title_full	Fusion of protein aggregates facilitates asymmetric damage segregation.
title_fullStr	Fusion of protein aggregates facilitates asymmetric damage segregation.
title_full_unstemmed	Fusion of protein aggregates facilitates asymmetric damage segregation.
title_sort	fusion of protein aggregates facilitates asymmetric damage segregation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/991e0c3fd5474dab9fbd13522abee3be
work_keys_str_mv	AT miguelcoelho fusionofproteinaggregatesfacilitatesasymmetricdamagesegregation AT stevenjlade fusionofproteinaggregatesfacilitatesasymmetricdamagesegregation AT simonalberti fusionofproteinaggregatesfacilitatesasymmetricdamagesegregation AT thilogross fusionofproteinaggregatesfacilitatesasymmetricdamagesegregation AT ivamtolic fusionofproteinaggregatesfacilitatesasymmetricdamagesegregation
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Fusion of protein aggregates facilitates asymmetric damage segregation.

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