Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Abstract We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sung-Hwan Choi, Won-Seok Jeong, Jung-Yul Cha, Jae-Hoon Lee, Kee-Joon Lee, Hyung-Seog Yu, Eun-Ha Choi, Kwang-Mahn Kim, Chung-Ju Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/992017047a69424f973b10c429fd9566
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:992017047a69424f973b10c429fd9566
record_format	dspace
spelling	oai:doaj.org-article:992017047a69424f973b10c429fd95662021-12-02T15:05:08ZOvercoming the biological aging of titanium using a wet storage method after ultraviolet treatment10.1038/s41598-017-04192-92045-2322https://doaj.org/article/992017047a69424f973b10c429fd95662017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04192-9https://doaj.org/toc/2045-2322Abstract We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H2O (dH2O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH2O for 28 days. We found that titanium stored in dH2O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH2O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.Sung-Hwan ChoiWon-Seok JeongJung-Yul ChaJae-Hoon LeeKee-Joon LeeHyung-Seog YuEun-Ha ChoiKwang-Mahn KimChung-Ju HwangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sung-Hwan Choi Won-Seok Jeong Jung-Yul Cha Jae-Hoon Lee Kee-Joon Lee Hyung-Seog Yu Eun-Ha Choi Kwang-Mahn Kim Chung-Ju Hwang Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
description	Abstract We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H2O (dH2O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH2O for 28 days. We found that titanium stored in dH2O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH2O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.
format	article
author	Sung-Hwan Choi Won-Seok Jeong Jung-Yul Cha Jae-Hoon Lee Kee-Joon Lee Hyung-Seog Yu Eun-Ha Choi Kwang-Mahn Kim Chung-Ju Hwang
author_facet	Sung-Hwan Choi Won-Seok Jeong Jung-Yul Cha Jae-Hoon Lee Kee-Joon Lee Hyung-Seog Yu Eun-Ha Choi Kwang-Mahn Kim Chung-Ju Hwang
author_sort	Sung-Hwan Choi
title	Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
title_short	Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
title_full	Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
title_fullStr	Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
title_full_unstemmed	Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
title_sort	overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/992017047a69424f973b10c429fd9566
work_keys_str_mv	AT sunghwanchoi overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT wonseokjeong overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT jungyulcha overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT jaehoonlee overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT keejoonlee overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT hyungseogyu overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT eunhachoi overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT kwangmahnkim overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment AT chungjuhwang overcomingthebiologicalagingoftitaniumusingawetstoragemethodafterultraviolettreatment
_version_	1718388908151013376

Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Ejemplares similares