Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.

The liver is one of the most sex-dimorphic organs in both oviparous and viviparous animals. In order to understand the molecular basis of the difference between male and female livers, high-throughput RNA-SAGE (serial analysis of gene expression) sequencing was performed for zebrafish livers of both...

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Autores principales: Weiling Zheng, Hongyan Xu, Siew Hong Lam, Huaien Luo, R Krishna Murthy Karuturi, Zhiyuan Gong
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:992348a077344948a7e7e326145305dc2021-11-18T08:01:07ZTranscriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.1932-620310.1371/journal.pone.0053562https://doaj.org/article/992348a077344948a7e7e326145305dc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349717/?tool=EBIhttps://doaj.org/toc/1932-6203The liver is one of the most sex-dimorphic organs in both oviparous and viviparous animals. In order to understand the molecular basis of the difference between male and female livers, high-throughput RNA-SAGE (serial analysis of gene expression) sequencing was performed for zebrafish livers of both sexes and their transcriptomes were compared. Both sexes had abundantly expressed genes involved in translation, coagulation and lipid metabolism, consistent with the general function of the liver. For sex-biased transcripts, from in addition to the high enrichment of vitellogenin transcripts in spawning female livers, which constituted nearly 80% of total mRNA, it is apparent that the female-biased genes were mostly involved in ribosome/translation, estrogen pathway, lipid transport, etc, while the male-biased genes were enriched for oxidation reduction, carbohydrate metabolism, coagulation, protein transport and localization, etc. Sexual dimorphism on xenobiotic metabolism and anti-oxidation was also noted and it is likely that retinol x receptor (RXR) and liver x receptor (LXR) play central roles in regulating the sexual differences of lipid and cholesterol metabolisms. Consistent with high ribosomal/translational activities in the female liver, female-biased genes were significantly regulated by two important transcription factors, Myc and Mycn. In contrast, Male livers showed activation of transcription factors Ppargc1b, Hnf4a, and Stat4, which regulate lipid and glucose metabolisms and various cellular activities. The transcriptomic responses to sex hormones, 17β-estradiol (E2) or 11-keto testosterone (KT11), were also investigated in both male and female livers and we found that female livers were relatively insensitive to sex hormone disturbance, while the male livers were readily affected. E2 feminized male liver by up-regulating female-biased transcripts and down-regulating male-biased transcripts. The information obtained in this study provides comprehensive insights into the sexual dimorphism of zebrafish liver transcriptome and will facilitate further development of the zebrafish as a human liver disease model.Weiling ZhengHongyan XuSiew Hong LamHuaien LuoR Krishna Murthy KaruturiZhiyuan GongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53562 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Weiling Zheng
Hongyan Xu
Siew Hong Lam
Huaien Luo
R Krishna Murthy Karuturi
Zhiyuan Gong
Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
description The liver is one of the most sex-dimorphic organs in both oviparous and viviparous animals. In order to understand the molecular basis of the difference between male and female livers, high-throughput RNA-SAGE (serial analysis of gene expression) sequencing was performed for zebrafish livers of both sexes and their transcriptomes were compared. Both sexes had abundantly expressed genes involved in translation, coagulation and lipid metabolism, consistent with the general function of the liver. For sex-biased transcripts, from in addition to the high enrichment of vitellogenin transcripts in spawning female livers, which constituted nearly 80% of total mRNA, it is apparent that the female-biased genes were mostly involved in ribosome/translation, estrogen pathway, lipid transport, etc, while the male-biased genes were enriched for oxidation reduction, carbohydrate metabolism, coagulation, protein transport and localization, etc. Sexual dimorphism on xenobiotic metabolism and anti-oxidation was also noted and it is likely that retinol x receptor (RXR) and liver x receptor (LXR) play central roles in regulating the sexual differences of lipid and cholesterol metabolisms. Consistent with high ribosomal/translational activities in the female liver, female-biased genes were significantly regulated by two important transcription factors, Myc and Mycn. In contrast, Male livers showed activation of transcription factors Ppargc1b, Hnf4a, and Stat4, which regulate lipid and glucose metabolisms and various cellular activities. The transcriptomic responses to sex hormones, 17β-estradiol (E2) or 11-keto testosterone (KT11), were also investigated in both male and female livers and we found that female livers were relatively insensitive to sex hormone disturbance, while the male livers were readily affected. E2 feminized male liver by up-regulating female-biased transcripts and down-regulating male-biased transcripts. The information obtained in this study provides comprehensive insights into the sexual dimorphism of zebrafish liver transcriptome and will facilitate further development of the zebrafish as a human liver disease model.
format article
author Weiling Zheng
Hongyan Xu
Siew Hong Lam
Huaien Luo
R Krishna Murthy Karuturi
Zhiyuan Gong
author_facet Weiling Zheng
Hongyan Xu
Siew Hong Lam
Huaien Luo
R Krishna Murthy Karuturi
Zhiyuan Gong
author_sort Weiling Zheng
title Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
title_short Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
title_full Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
title_fullStr Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
title_full_unstemmed Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
title_sort transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/992348a077344948a7e7e326145305dc
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