Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor

COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, ea...

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Autores principales:	Tian-Rui Cui, Yan-Cong Qiao, Jian-Wei Gao, Chun-Hua Wang, Yu Zhang, Lin Han, Yi Yang, Tian-Ling Ren
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	LIG-FET biosensor COVID-19 SARS-CoV-2 flexible devices Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/9925b74b535e4cdda9cd4df47cef7f52
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spelling	oai:doaj.org-article:9925b74b535e4cdda9cd4df47cef7f522021-11-25T18:28:39ZUltrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor10.3390/molecules262269471420-3049https://doaj.org/article/9925b74b535e4cdda9cd4df47cef7f522021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6947https://doaj.org/toc/1420-3049COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, early diagnosis and isolation are essential to control the outbreak. Current nucleic acid testing methods require high sample quality and laboratory conditions, which cannot meet flexible applications. Here, we report a laser-induced graphene field-effect transistor (LIG-FET) for detecting SARS-CoV-2. The FET was manufactured by different reduction degree LIG, with an oyster reef-like porous graphene channel to enrich the binding point between the virus protein and sensing area. After immobilizing specific antibodies in the channel, the FET can detect the SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL in human serum. In addition, the sensor shows great specificity to the spike protein of SARS-CoV-2. Our sensors can realize fast production for COVID-19 rapid testing, as each LIG-FET can be fabricated by a laser platform in seconds. It is the first time that LIG has realized a virus sensing FET without any sample pretreatment or labeling, which paves the way for low-cost and rapid detection of COVID-19.Tian-Rui CuiYan-Cong QiaoJian-Wei GaoChun-Hua WangYu ZhangLin HanYi YangTian-Ling RenMDPI AGarticleLIG-FETbiosensorCOVID-19SARS-CoV-2flexible devicesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6947, p 6947 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	LIG-FET biosensor COVID-19 SARS-CoV-2 flexible devices Organic chemistry QD241-441
spellingShingle	LIG-FET biosensor COVID-19 SARS-CoV-2 flexible devices Organic chemistry QD241-441 Tian-Rui Cui Yan-Cong Qiao Jian-Wei Gao Chun-Hua Wang Yu Zhang Lin Han Yi Yang Tian-Ling Ren Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
description	COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, early diagnosis and isolation are essential to control the outbreak. Current nucleic acid testing methods require high sample quality and laboratory conditions, which cannot meet flexible applications. Here, we report a laser-induced graphene field-effect transistor (LIG-FET) for detecting SARS-CoV-2. The FET was manufactured by different reduction degree LIG, with an oyster reef-like porous graphene channel to enrich the binding point between the virus protein and sensing area. After immobilizing specific antibodies in the channel, the FET can detect the SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL in human serum. In addition, the sensor shows great specificity to the spike protein of SARS-CoV-2. Our sensors can realize fast production for COVID-19 rapid testing, as each LIG-FET can be fabricated by a laser platform in seconds. It is the first time that LIG has realized a virus sensing FET without any sample pretreatment or labeling, which paves the way for low-cost and rapid detection of COVID-19.
format	article
author	Tian-Rui Cui Yan-Cong Qiao Jian-Wei Gao Chun-Hua Wang Yu Zhang Lin Han Yi Yang Tian-Ling Ren
author_facet	Tian-Rui Cui Yan-Cong Qiao Jian-Wei Gao Chun-Hua Wang Yu Zhang Lin Han Yi Yang Tian-Ling Ren
author_sort	Tian-Rui Cui
title	Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
title_short	Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
title_full	Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
title_fullStr	Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
title_full_unstemmed	Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor
title_sort	ultrasensitive detection of covid-19 causative virus (sars-cov-2) spike protein using laser induced graphene field-effect transistor
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9925b74b535e4cdda9cd4df47cef7f52
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Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor

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