Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae

Abstract Streptococcus pneumoniae invades the CNS and triggers a strong cellular response. To date, signaling events that occur in the human brain microvascular endothelial cells (hBMECs), in response to pneumococci or its surface adhesins are not mapped comprehensively. We evaluated the response of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Irene Jiménez-Munguía, Zuzana Tomečková, Evelína Mochnáčová, Katarína Bhide, Petra Majerová, Mangesh Bhide
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9929454e434e49b581ccbf346688fd2f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9929454e434e49b581ccbf346688fd2f
record_format	dspace
spelling	oai:doaj.org-article:9929454e434e49b581ccbf346688fd2f2021-12-02T18:02:57ZTranscriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae10.1038/s41598-021-87021-42045-2322https://doaj.org/article/9929454e434e49b581ccbf346688fd2f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87021-4https://doaj.org/toc/2045-2322Abstract Streptococcus pneumoniae invades the CNS and triggers a strong cellular response. To date, signaling events that occur in the human brain microvascular endothelial cells (hBMECs), in response to pneumococci or its surface adhesins are not mapped comprehensively. We evaluated the response of hBMECs to the adhesion lipoprotein (a laminin binding protein—Lbp) or live pneumococci. Lbp is a surface adhesin recently identified as a potential ligand, which binds to the hBMECs. Transcriptomic analysis was performed by RNA-seq of three independent biological replicates and validated with qRT-PCR using 11 genes. In total 350 differentially expressed genes (DEGs) were identified after infection with S. pneumoniae, whereas 443 DEGs when challenged with Lbp. Total 231 DEGs were common in both treatments. Integrative functional analysis revealed participation of DEGs in cytokine, chemokine, TNF signaling pathways and phagosome formation. Moreover, Lbp induced cell senescence and breakdown, and remodeling of ECM. This is the first report which maps complete picture of cell signaling events in the hBMECs triggered against S. pneumoniae and Lbp. The data obtained here could contribute in a better understanding of the invasion of pneumococci across BBB and underscores role of Lbp adhesin in evoking the gene expression in neurovascular unit.Irene Jiménez-MunguíaZuzana TomečkováEvelína MochnáčováKatarína BhidePetra MajerováMangesh BhideNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irene Jiménez-Munguía Zuzana Tomečková Evelína Mochnáčová Katarína Bhide Petra Majerová Mangesh Bhide Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
description	Abstract Streptococcus pneumoniae invades the CNS and triggers a strong cellular response. To date, signaling events that occur in the human brain microvascular endothelial cells (hBMECs), in response to pneumococci or its surface adhesins are not mapped comprehensively. We evaluated the response of hBMECs to the adhesion lipoprotein (a laminin binding protein—Lbp) or live pneumococci. Lbp is a surface adhesin recently identified as a potential ligand, which binds to the hBMECs. Transcriptomic analysis was performed by RNA-seq of three independent biological replicates and validated with qRT-PCR using 11 genes. In total 350 differentially expressed genes (DEGs) were identified after infection with S. pneumoniae, whereas 443 DEGs when challenged with Lbp. Total 231 DEGs were common in both treatments. Integrative functional analysis revealed participation of DEGs in cytokine, chemokine, TNF signaling pathways and phagosome formation. Moreover, Lbp induced cell senescence and breakdown, and remodeling of ECM. This is the first report which maps complete picture of cell signaling events in the hBMECs triggered against S. pneumoniae and Lbp. The data obtained here could contribute in a better understanding of the invasion of pneumococci across BBB and underscores role of Lbp adhesin in evoking the gene expression in neurovascular unit.
format	article
author	Irene Jiménez-Munguía Zuzana Tomečková Evelína Mochnáčová Katarína Bhide Petra Majerová Mangesh Bhide
author_facet	Irene Jiménez-Munguía Zuzana Tomečková Evelína Mochnáčová Katarína Bhide Petra Majerová Mangesh Bhide
author_sort	Irene Jiménez-Munguía
title	Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
title_short	Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
title_full	Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
title_fullStr	Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
title_full_unstemmed	Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae
title_sort	transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and streptococcus pneumoniae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9929454e434e49b581ccbf346688fd2f
work_keys_str_mv	AT irenejimenezmunguia transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae AT zuzanatomeckova transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae AT evelinamochnacova transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae AT katarinabhide transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae AT petramajerova transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae AT mangeshbhide transcriptomicanalysisofhumanbrainmicrovascularendothelialcellsexposedtolamininbindingproteinadhesionlipoproteinandstreptococcuspneumoniae
_version_	1718378823875035136

Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae

Ejemplares similares