A biomimetic engineered bone platform for advanced testing of prosthetic implants

Abstract Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advan...

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Autores principales:	Martina Sladkova-Faure, Michael Pujari-Palmer, Caroline Öhman-Mägi, Alejandro López, Hanbin Wang, Håkan Engqvist, Giuseppe Maria de Peppo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/992c43244ca644bbb1a7810e08b13767
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spelling	oai:doaj.org-article:992c43244ca644bbb1a7810e08b137672021-12-02T12:03:15ZA biomimetic engineered bone platform for advanced testing of prosthetic implants10.1038/s41598-020-78416-w2045-2322https://doaj.org/article/992c43244ca644bbb1a7810e08b137672020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78416-whttps://doaj.org/toc/2045-2322Abstract Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advanced testing of implants in vitro, and demonstrate the scientific validity and predictive value of this approach using an assortment of complementary evaluation methods. We anchored titanium (Ti) and stainless steel (SS) implants into biomimetic scaffolds, seeded with human induced mesenchymal stem cells, to recapitulate the osseointegration process in vitro. We show distinct patterns of gene expression, matrix deposition, and mineralization in response to the two materials, with Ti implants ultimately resulting in stronger integration strength, as seen in other preclinical and clinical studies. Interestingly, RNAseq analysis reveals that the TGF-beta and the FGF2 pathways are overexpressed in response to Ti implants, while the Wnt, BMP, and IGF pathways are overexpressed in response to SS implants. High-resolution imaging shows significantly increased tissue mineralization and calcium deposition at the tissue-implant interface in response to Ti implants, contributing to a twofold increase in pullout strength compared to SS implants. Our technology creates unprecedented research opportunities towards the design of implants and biomaterials that can be personalized, and exhibit enhanced osseointegration potential, with reduced need for animal testing.Martina Sladkova-FaureMichael Pujari-PalmerCaroline Öhman-MägiAlejandro LópezHanbin WangHåkan EngqvistGiuseppe Maria de PeppoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Sladkova-Faure Michael Pujari-Palmer Caroline Öhman-Mägi Alejandro López Hanbin Wang Håkan Engqvist Giuseppe Maria de Peppo A biomimetic engineered bone platform for advanced testing of prosthetic implants
description	Abstract Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advanced testing of implants in vitro, and demonstrate the scientific validity and predictive value of this approach using an assortment of complementary evaluation methods. We anchored titanium (Ti) and stainless steel (SS) implants into biomimetic scaffolds, seeded with human induced mesenchymal stem cells, to recapitulate the osseointegration process in vitro. We show distinct patterns of gene expression, matrix deposition, and mineralization in response to the two materials, with Ti implants ultimately resulting in stronger integration strength, as seen in other preclinical and clinical studies. Interestingly, RNAseq analysis reveals that the TGF-beta and the FGF2 pathways are overexpressed in response to Ti implants, while the Wnt, BMP, and IGF pathways are overexpressed in response to SS implants. High-resolution imaging shows significantly increased tissue mineralization and calcium deposition at the tissue-implant interface in response to Ti implants, contributing to a twofold increase in pullout strength compared to SS implants. Our technology creates unprecedented research opportunities towards the design of implants and biomaterials that can be personalized, and exhibit enhanced osseointegration potential, with reduced need for animal testing.
format	article
author	Martina Sladkova-Faure Michael Pujari-Palmer Caroline Öhman-Mägi Alejandro López Hanbin Wang Håkan Engqvist Giuseppe Maria de Peppo
author_facet	Martina Sladkova-Faure Michael Pujari-Palmer Caroline Öhman-Mägi Alejandro López Hanbin Wang Håkan Engqvist Giuseppe Maria de Peppo
author_sort	Martina Sladkova-Faure
title	A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_short	A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_full	A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_fullStr	A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_full_unstemmed	A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_sort	biomimetic engineered bone platform for advanced testing of prosthetic implants
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/992c43244ca644bbb1a7810e08b13767
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A biomimetic engineered bone platform for advanced testing of prosthetic implants

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