Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.

Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ergosterol, on the metabolome of cultured tobacco cells. Cel...

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Autores principales:	Fidele Tugizimana, Paul A Steenkamp, Lizelle A Piater, Ian A Dubery
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99336450a5464f3fae5206b3f666cbee
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spelling	oai:doaj.org-article:99336450a5464f3fae5206b3f666cbee2021-11-18T08:34:26ZMulti-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.1932-620310.1371/journal.pone.0087846https://doaj.org/article/99336450a5464f3fae5206b3f666cbee2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498209/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ergosterol, on the metabolome of cultured tobacco cells. Cell suspensions were treated with different concentrations (0-1000 nM) of ergosterol and incubated for different time periods (0-24 h). Intracellular metabolites were extracted with two methods: a selective dispersive liquid-liquid micro-extraction and a general methanol extraction. Chromatographic techniques (GC-FID, GC-MS, GC × GC-TOF-MS, UHPLC-MS) and (1)H NMR spectroscopy were used for quantitative and qualitative analyses. Multivariate data analyses (PCA and OPLS-DA models) were used to extract interpretable information from the multidimensional data generated from the analytical techniques. The results showed that ergosterol triggered differential changes in the metabolome of the cells, leading to variation in the biosynthesis of secondary metabolites. PCA scores plots revealed dose- and time-dependent metabolic variations, with optimal treatment conditions being found to be 300 nM ergosterol and an 18 h incubation period. The observed ergosterol-induced metabolic changes were correlated with changes in defence-related metabolites. The 'defensome' involved increases in terpenoid metabolites with five antimicrobial compounds (the bicyclic sesquiterpenoid phytoalexins: phytuberin, solavetivone, capsidiol, lubimin and rishitin) and other metabolites (abscisic acid and phytosterols) putatively identified. In addition, various phenylpropanoid precursors, cinnamic acid derivatives and - conjugates, coumarins and lignin monomers were annotated. These annotated metabolites revealed a dynamic reprogramming of metabolic networks that are functionally correlated, with a high complexity in their regulation.Fidele TugizimanaPaul A SteenkampLizelle A PiaterIan A DuberyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87846 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fidele Tugizimana Paul A Steenkamp Lizelle A Piater Ian A Dubery Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
description	Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ergosterol, on the metabolome of cultured tobacco cells. Cell suspensions were treated with different concentrations (0-1000 nM) of ergosterol and incubated for different time periods (0-24 h). Intracellular metabolites were extracted with two methods: a selective dispersive liquid-liquid micro-extraction and a general methanol extraction. Chromatographic techniques (GC-FID, GC-MS, GC × GC-TOF-MS, UHPLC-MS) and (1)H NMR spectroscopy were used for quantitative and qualitative analyses. Multivariate data analyses (PCA and OPLS-DA models) were used to extract interpretable information from the multidimensional data generated from the analytical techniques. The results showed that ergosterol triggered differential changes in the metabolome of the cells, leading to variation in the biosynthesis of secondary metabolites. PCA scores plots revealed dose- and time-dependent metabolic variations, with optimal treatment conditions being found to be 300 nM ergosterol and an 18 h incubation period. The observed ergosterol-induced metabolic changes were correlated with changes in defence-related metabolites. The 'defensome' involved increases in terpenoid metabolites with five antimicrobial compounds (the bicyclic sesquiterpenoid phytoalexins: phytuberin, solavetivone, capsidiol, lubimin and rishitin) and other metabolites (abscisic acid and phytosterols) putatively identified. In addition, various phenylpropanoid precursors, cinnamic acid derivatives and - conjugates, coumarins and lignin monomers were annotated. These annotated metabolites revealed a dynamic reprogramming of metabolic networks that are functionally correlated, with a high complexity in their regulation.
format	article
author	Fidele Tugizimana Paul A Steenkamp Lizelle A Piater Ian A Dubery
author_facet	Fidele Tugizimana Paul A Steenkamp Lizelle A Piater Ian A Dubery
author_sort	Fidele Tugizimana
title	Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
title_short	Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
title_full	Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
title_fullStr	Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
title_full_unstemmed	Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.
title_sort	multi-platform metabolomic analyses of ergosterol-induced dynamic changes in nicotiana tabacum cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/99336450a5464f3fae5206b3f666cbee
work_keys_str_mv	AT fideletugizimana multiplatformmetabolomicanalysesofergosterolinduceddynamicchangesinnicotianatabacumcells AT paulasteenkamp multiplatformmetabolomicanalysesofergosterolinduceddynamicchangesinnicotianatabacumcells AT lizelleapiater multiplatformmetabolomicanalysesofergosterolinduceddynamicchangesinnicotianatabacumcells AT ianadubery multiplatformmetabolomicanalysesofergosterolinduceddynamicchangesinnicotianatabacumcells
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Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells.

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