Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage

Abstract Repetitive transorbital alternating current stimulation (rtACS) improves vision in patients with chronic visual impairments and an acute treatment increased survival of retinal neurons after optic nerve crush (ONC) in rodent models of visual system injury. However, despite this protection n...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Petra Henrich-Noack, Elena G. Sergeeva, Torben Eber, Qing You, Nadine Voigt, Jürgen Köhler, Sebastian Wagner, Stefanie Lazik, Christian Mawrin, Guihua Xu, Sayantan Biswas, Bernhard A. Sabel, Christopher Kai-Shun Leung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99340675faf845f2b6ab9a12b0e4221a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:99340675faf845f2b6ab9a12b0e4221a
record_format	dspace
spelling	oai:doaj.org-article:99340675faf845f2b6ab9a12b0e4221a2021-12-02T12:30:17ZElectrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage10.1038/s41598-017-00487-z2045-2322https://doaj.org/article/99340675faf845f2b6ab9a12b0e4221a2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00487-zhttps://doaj.org/toc/2045-2322Abstract Repetitive transorbital alternating current stimulation (rtACS) improves vision in patients with chronic visual impairments and an acute treatment increased survival of retinal neurons after optic nerve crush (ONC) in rodent models of visual system injury. However, despite this protection no functional recovery could be detected in rats, which was interpreted as evidence of “silent survivor” cells. We now analysed the mechanisms underlying this “silent survival” effect. Using in vivo microscopy of the retina we investigated the survival and morphology of fluorescent neurons before and after ONC in animals receiving rtACS or sham treatment. One week after the crush, more neurons survived in the rtACS-treated group compared to sham-treated controls. In vivo imaging further revealed that in the initial post-ONC period, rtACS induced dendritic pruning in surviving neurons. In contrast, dendrites in untreated retinae degenerated slowly after the axonal trauma and neurons died. The complete loss of visual evoked potentials supports the hypothesis that cell signalling is abolished in the surviving neurons. Despite this evidence of “silencing”, intracellular free calcium imaging showed that the cells were still viable. We propose that early after trauma, complete dendritic stripping following rtACS protects neurons from excitotoxic cell death by silencing them.Petra Henrich-NoackElena G. SergeevaTorben EberQing YouNadine VoigtJürgen KöhlerSebastian WagnerStefanie LazikChristian MawrinGuihua XuSayantan BiswasBernhard A. SabelChristopher Kai-Shun LeungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Petra Henrich-Noack Elena G. Sergeeva Torben Eber Qing You Nadine Voigt Jürgen Köhler Sebastian Wagner Stefanie Lazik Christian Mawrin Guihua Xu Sayantan Biswas Bernhard A. Sabel Christopher Kai-Shun Leung Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
description	Abstract Repetitive transorbital alternating current stimulation (rtACS) improves vision in patients with chronic visual impairments and an acute treatment increased survival of retinal neurons after optic nerve crush (ONC) in rodent models of visual system injury. However, despite this protection no functional recovery could be detected in rats, which was interpreted as evidence of “silent survivor” cells. We now analysed the mechanisms underlying this “silent survival” effect. Using in vivo microscopy of the retina we investigated the survival and morphology of fluorescent neurons before and after ONC in animals receiving rtACS or sham treatment. One week after the crush, more neurons survived in the rtACS-treated group compared to sham-treated controls. In vivo imaging further revealed that in the initial post-ONC period, rtACS induced dendritic pruning in surviving neurons. In contrast, dendrites in untreated retinae degenerated slowly after the axonal trauma and neurons died. The complete loss of visual evoked potentials supports the hypothesis that cell signalling is abolished in the surviving neurons. Despite this evidence of “silencing”, intracellular free calcium imaging showed that the cells were still viable. We propose that early after trauma, complete dendritic stripping following rtACS protects neurons from excitotoxic cell death by silencing them.
format	article
author	Petra Henrich-Noack Elena G. Sergeeva Torben Eber Qing You Nadine Voigt Jürgen Köhler Sebastian Wagner Stefanie Lazik Christian Mawrin Guihua Xu Sayantan Biswas Bernhard A. Sabel Christopher Kai-Shun Leung
author_facet	Petra Henrich-Noack Elena G. Sergeeva Torben Eber Qing You Nadine Voigt Jürgen Köhler Sebastian Wagner Stefanie Lazik Christian Mawrin Guihua Xu Sayantan Biswas Bernhard A. Sabel Christopher Kai-Shun Leung
author_sort	Petra Henrich-Noack
title	Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
title_short	Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
title_full	Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
title_fullStr	Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
title_full_unstemmed	Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
title_sort	electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/99340675faf845f2b6ab9a12b0e4221a
work_keys_str_mv	AT petrahenrichnoack electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT elenagsergeeva electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT torbeneber electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT qingyou electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT nadinevoigt electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT jurgenkohler electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT sebastianwagner electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT stefanielazik electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT christianmawrin electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT guihuaxu electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT sayantanbiswas electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT bernhardasabel electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage AT christopherkaishunleung electricalbrainstimulationinducesdendriticstrippingbutimprovessurvivalofsilentneuronsafteropticnervedamage
_version_	1718394411180621824

Electrical brain stimulation induces dendritic stripping but improves survival of silent neurons after optic nerve damage

Ejemplares similares