Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections

Xu Xu,* Zhaokang Xu,* Junyi Liu, Zhaoliang Zhang, Hao Chen, Xingyi Li, Shuai Shi Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China *These authors contributed equally...

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Autores principales:	Xu X, Xu ZK, Liu JY, Zhang ZL, Chen H, Li XY, Shi S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	photoacoustic imaging intravitreal injection amphiphilic cationic nanoparticle tracing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/99359971f0ac46b79a7ae2013fe525d2
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spelling	oai:doaj.org-article:99359971f0ac46b79a7ae2013fe525d22021-12-02T03:11:42ZVisual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections1178-2013https://doaj.org/article/99359971f0ac46b79a7ae2013fe525d22016-10-01T00:00:00Zhttps://www.dovepress.com/visual-tracing-of-diffusion-and-biodistribution-for-amphiphilic-cation-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xu Xu,* Zhaokang Xu,* Junyi Liu, Zhaoliang Zhang, Hao Chen, Xingyi Li, Shuai Shi Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China *These authors contributed equally to this work Abstract: To visually trace the diffusion and biodistribution of amphiphilic cation micelles after vitreous injection, various triblock copolymers of monomethoxy poly(ethylene glycol)–poly(ε-caprolactone)–polyethylenimine were synthesized with different structures of hydrophilic and hydrophobic segments, followed by labeling with near-infrared fluorescent dye Cyanine5 or Cyanine7. The micellar size, polydispersity index, and surface charge were measured by dynamic light scattering. The diffusion was monitored using photoacoustic imaging in real time after intravitreal injections. Moreover, the labeled nanoparticle distribution in the posterior segment of the eye was imaged histologically by confocal microscopy. The results showed that the hydrophilic segment increased vitreous diffusion, while a positive charge on the particle surface hindered diffusion. In addition, the particles diffused through the retinal layers and were enriched in the retinal pigment epithelial layer. This work tried to study the diffusion rate via a simple method by using visible images, and then provided basic data for the development of intraocular drug carriers. Keywords: visible tracing, cavum vitreum, biodistribution, diffusion rateXu XXu ZKLiu JYZhang ZLChen HLi XYShi SDove Medical Pressarticlephotoacoustic imagingintravitreal injectionamphiphilic cationic nanoparticletracingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5079-5086 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	photoacoustic imaging intravitreal injection amphiphilic cationic nanoparticle tracing Medicine (General) R5-920
spellingShingle	photoacoustic imaging intravitreal injection amphiphilic cationic nanoparticle tracing Medicine (General) R5-920 Xu X Xu ZK Liu JY Zhang ZL Chen H Li XY Shi S Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
description	Xu Xu,* Zhaokang Xu,* Junyi Liu, Zhaoliang Zhang, Hao Chen, Xingyi Li, Shuai Shi Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China *These authors contributed equally to this work Abstract: To visually trace the diffusion and biodistribution of amphiphilic cation micelles after vitreous injection, various triblock copolymers of monomethoxy poly(ethylene glycol)–poly(ε-caprolactone)–polyethylenimine were synthesized with different structures of hydrophilic and hydrophobic segments, followed by labeling with near-infrared fluorescent dye Cyanine5 or Cyanine7. The micellar size, polydispersity index, and surface charge were measured by dynamic light scattering. The diffusion was monitored using photoacoustic imaging in real time after intravitreal injections. Moreover, the labeled nanoparticle distribution in the posterior segment of the eye was imaged histologically by confocal microscopy. The results showed that the hydrophilic segment increased vitreous diffusion, while a positive charge on the particle surface hindered diffusion. In addition, the particles diffused through the retinal layers and were enriched in the retinal pigment epithelial layer. This work tried to study the diffusion rate via a simple method by using visible images, and then provided basic data for the development of intraocular drug carriers. Keywords: visible tracing, cavum vitreum, biodistribution, diffusion rate
format	article
author	Xu X Xu ZK Liu JY Zhang ZL Chen H Li XY Shi S
author_facet	Xu X Xu ZK Liu JY Zhang ZL Chen H Li XY Shi S
author_sort	Xu X
title	Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
title_short	Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
title_full	Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
title_fullStr	Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
title_full_unstemmed	Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
title_sort	visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/99359971f0ac46b79a7ae2013fe525d2
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_version_	1718401872605216768

Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections

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