The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine

The hypotensive and antihypertensive activities of the aqueous extract (AE) and butanolic fraction (ButF) isolated from Cecropia glaziovii Sneth have been demonstrated in previous studies in animal models. This study aimed to evaluate the molecular mechanism of action responsible for the vasodilator...

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Autores principales:	Gabriella Trettel, Clelia Rejane Antonio Bertoncini, Maria Teresa Lima-Landman
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Antihypertensive, Ca2+ channels Contractile antagonists Herbal medicines Hypertension Rat Respiratory diseases Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/99389ec851c3464fbbffc9881f0e5cec
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spelling	oai:doaj.org-article:99389ec851c3464fbbffc9881f0e5cec2021-11-14T04:28:43ZThe mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine0753-332210.1016/j.biopha.2021.112231https://doaj.org/article/99389ec851c3464fbbffc9881f0e5cec2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221010155https://doaj.org/toc/0753-3322The hypotensive and antihypertensive activities of the aqueous extract (AE) and butanolic fraction (ButF) isolated from Cecropia glaziovii Sneth have been demonstrated in previous studies in animal models. This study aimed to evaluate the molecular mechanism of action responsible for the vasodilatory effect of procyanidins, flavanols, and flavonoids found in C. glaziovii in endothelial cell culture. For this purpose, we analyzed the effect of procyanidin B2 and B3 compounds, catechin, epicatechin, orientin, isoorientin, and isovitexin in the mobilization of Ca2+ in rat endothelial cell cultures. Parallel associations with different antagonists were examined by considering the following in vivo hypotensive mechanisms: blockage of L-type calcium channels, action on β-2 adrenergic receptors, and vasodilation via the nitric oxide pathway. All measurements of calcium mobilization were carried out by using the fluorescence measurement methodology in a Flexstation M3 spectrophotometer. The results indicate that some of the compounds have mixed actions, acting through different calcium mobilization pathways. The mobilization induced by such compounds significantly decreased when they were incubated with their corresponding antagonists. Taken together, our data suggest that the beneficial effects seen with the popular use of Cecropia glaziovii Sneth in pathological conditions, such as systemic arterial hypertension, seem to be related to the plant's hypotensive effect, very probably promoted by the actions of flavonols, flavonoids, and procyanidins, by different pathways of calcium mobilization.Gabriella TrettelClelia Rejane Antonio BertonciniMaria Teresa Lima-LandmanElsevierarticleAntihypertensive, Ca2+ channelsContractile antagonistsHerbal medicinesHypertensionRatRespiratory diseasesTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112231- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antihypertensive, Ca2+ channels Contractile antagonists Herbal medicines Hypertension Rat Respiratory diseases Therapeutics. Pharmacology RM1-950
spellingShingle	Antihypertensive, Ca2+ channels Contractile antagonists Herbal medicines Hypertension Rat Respiratory diseases Therapeutics. Pharmacology RM1-950 Gabriella Trettel Clelia Rejane Antonio Bertoncini Maria Teresa Lima-Landman The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
description	The hypotensive and antihypertensive activities of the aqueous extract (AE) and butanolic fraction (ButF) isolated from Cecropia glaziovii Sneth have been demonstrated in previous studies in animal models. This study aimed to evaluate the molecular mechanism of action responsible for the vasodilatory effect of procyanidins, flavanols, and flavonoids found in C. glaziovii in endothelial cell culture. For this purpose, we analyzed the effect of procyanidin B2 and B3 compounds, catechin, epicatechin, orientin, isoorientin, and isovitexin in the mobilization of Ca2+ in rat endothelial cell cultures. Parallel associations with different antagonists were examined by considering the following in vivo hypotensive mechanisms: blockage of L-type calcium channels, action on β-2 adrenergic receptors, and vasodilation via the nitric oxide pathway. All measurements of calcium mobilization were carried out by using the fluorescence measurement methodology in a Flexstation M3 spectrophotometer. The results indicate that some of the compounds have mixed actions, acting through different calcium mobilization pathways. The mobilization induced by such compounds significantly decreased when they were incubated with their corresponding antagonists. Taken together, our data suggest that the beneficial effects seen with the popular use of Cecropia glaziovii Sneth in pathological conditions, such as systemic arterial hypertension, seem to be related to the plant's hypotensive effect, very probably promoted by the actions of flavonols, flavonoids, and procyanidins, by different pathways of calcium mobilization.
format	article
author	Gabriella Trettel Clelia Rejane Antonio Bertoncini Maria Teresa Lima-Landman
author_facet	Gabriella Trettel Clelia Rejane Antonio Bertoncini Maria Teresa Lima-Landman
author_sort	Gabriella Trettel
title	The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
title_short	The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
title_full	The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
title_fullStr	The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
title_full_unstemmed	The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
title_sort	mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from cecropia glaziovii sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/99389ec851c3464fbbffc9881f0e5cec
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The mechanisms of calcium mobilization by procyanidins, flavonols and flavonoids from Cecropia glaziovii Sneth in pulmonary endothelial cell cultures endorse its popular use as vasodilator phytomedicine

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