First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil

Abstract Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microspori...

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Autores principales:	Carolina Jordan, Vanessa Rafaela de Carvalho, Gabriel Moura Mascarin, Leiliane Rodrigues dos Santos Oliveira, Christopher A. Dunlap, Carlos Frederico Wilcken
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/993bfd3f01bc452dbae564b576e21fe7
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spelling	oai:doaj.org-article:993bfd3f01bc452dbae564b576e21fe72021-12-02T16:53:11ZFirst record of a new microsporidium pathogenic to Gonipterus platensis in Brazil10.1038/s41598-021-90041-92045-2322https://doaj.org/article/993bfd3f01bc452dbae564b576e21fe72021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90041-9https://doaj.org/toc/2045-2322Abstract Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1 (± 0.4) × 107 spores/beetle. Symptoms in infected adults were identified by an abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~ 94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen of this invasive pest in Eucalyptus plantations in Brazil. Further studies are needed to determine potential of this microsporidium in the design of conservative or augmentative biological control programs for this invasive pest.Carolina JordanVanessa Rafaela de CarvalhoGabriel Moura MascarinLeiliane Rodrigues dos Santos OliveiraChristopher A. DunlapCarlos Frederico WilckenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Jordan Vanessa Rafaela de Carvalho Gabriel Moura Mascarin Leiliane Rodrigues dos Santos Oliveira Christopher A. Dunlap Carlos Frederico Wilcken First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
description	Abstract Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1 (± 0.4) × 107 spores/beetle. Symptoms in infected adults were identified by an abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~ 94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen of this invasive pest in Eucalyptus plantations in Brazil. Further studies are needed to determine potential of this microsporidium in the design of conservative or augmentative biological control programs for this invasive pest.
format	article
author	Carolina Jordan Vanessa Rafaela de Carvalho Gabriel Moura Mascarin Leiliane Rodrigues dos Santos Oliveira Christopher A. Dunlap Carlos Frederico Wilcken
author_facet	Carolina Jordan Vanessa Rafaela de Carvalho Gabriel Moura Mascarin Leiliane Rodrigues dos Santos Oliveira Christopher A. Dunlap Carlos Frederico Wilcken
author_sort	Carolina Jordan
title	First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
title_short	First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
title_full	First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
title_fullStr	First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
title_full_unstemmed	First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
title_sort	first record of a new microsporidium pathogenic to gonipterus platensis in brazil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/993bfd3f01bc452dbae564b576e21fe7
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First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil

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