Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide

Abstract RNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function in diverse taxa including arthropods for both basic biological research and application in pest control. The conservation of the RNAi mechanism in eukaryotes suggested that it should—in principle—be appli...

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Autores principales:	Sonja Mehlhorn, Vera S. Hunnekuhl, Sven Geibel, Ralf Nauen, Gregor Bucher
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	RNA interference RNAi Establishment of RNAi Off target control Controls Variability Zoology QL1-991
Acceso en línea:	https://doaj.org/article/99412be0fb8843278e62e1a3e7d56559
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spelling	oai:doaj.org-article:99412be0fb8843278e62e1a3e7d565592021-12-05T12:18:43ZEstablishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide10.1186/s12983-021-00444-71742-9994https://doaj.org/article/99412be0fb8843278e62e1a3e7d565592021-12-01T00:00:00Zhttps://doi.org/10.1186/s12983-021-00444-7https://doaj.org/toc/1742-9994Abstract RNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function in diverse taxa including arthropods for both basic biological research and application in pest control. The conservation of the RNAi mechanism in eukaryotes suggested that it should—in principle—be applicable to most arthropods. However, practical hurdles have been limiting the application in many taxa. For instance, species differ considerably with respect to efficiency of dsRNA uptake from the hemolymph or the gut. Here, we review some of the most frequently encountered technical obstacles when establishing RNAi and suggest a robust procedure for establishing this technique in insect species with special reference to pests. Finally, we present an approach to identify the most effective target genes for the potential control of agricultural and public health pests by RNAi.Sonja MehlhornVera S. HunnekuhlSven GeibelRalf NauenGregor BucherBMCarticleRNA interferenceRNAiEstablishment of RNAiOff target controlControlsVariabilityZoologyQL1-991ENFrontiers in Zoology, Vol 18, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA interference RNAi Establishment of RNAi Off target control Controls Variability Zoology QL1-991
spellingShingle	RNA interference RNAi Establishment of RNAi Off target control Controls Variability Zoology QL1-991 Sonja Mehlhorn Vera S. Hunnekuhl Sven Geibel Ralf Nauen Gregor Bucher Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
description	Abstract RNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function in diverse taxa including arthropods for both basic biological research and application in pest control. The conservation of the RNAi mechanism in eukaryotes suggested that it should—in principle—be applicable to most arthropods. However, practical hurdles have been limiting the application in many taxa. For instance, species differ considerably with respect to efficiency of dsRNA uptake from the hemolymph or the gut. Here, we review some of the most frequently encountered technical obstacles when establishing RNAi and suggest a robust procedure for establishing this technique in insect species with special reference to pests. Finally, we present an approach to identify the most effective target genes for the potential control of agricultural and public health pests by RNAi.
format	article
author	Sonja Mehlhorn Vera S. Hunnekuhl Sven Geibel Ralf Nauen Gregor Bucher
author_facet	Sonja Mehlhorn Vera S. Hunnekuhl Sven Geibel Ralf Nauen Gregor Bucher
author_sort	Sonja Mehlhorn
title	Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
title_short	Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
title_full	Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
title_fullStr	Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
title_full_unstemmed	Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
title_sort	establishing rnai for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide
publisher	BMC
publishDate	2021
url	https://doaj.org/article/99412be0fb8843278e62e1a3e7d56559
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Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide

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