Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.

Gastric cancer is the second leading cause of cancer-related death worldwide. The identification of new cancer biomarkers is necessary to reduce the mortality rates through the development of new screening assays and early diagnosis, as well as new target therapies. In this study, we performed a pro...

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Autores principales:	Mariana Ferreira Leal, Janete Chung, Danielle Queiroz Calcagno, Paulo Pimentel Assumpção, Samia Demachki, Ismael Dale Cotrim Guerreiro da Silva, Roger Chammas, Rommel Rodríguez Burbano, Marília de Arruda Cardoso Smith
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99414cf4f7884f95bff806d2d113c9f6
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spelling	oai:doaj.org-article:99414cf4f7884f95bff806d2d113c9f62021-11-18T07:10:21ZDifferential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.1932-620310.1371/journal.pone.0042255https://doaj.org/article/99414cf4f7884f95bff806d2d113c9f62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22860099/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Gastric cancer is the second leading cause of cancer-related death worldwide. The identification of new cancer biomarkers is necessary to reduce the mortality rates through the development of new screening assays and early diagnosis, as well as new target therapies. In this study, we performed a proteomic analysis of noncardia gastric neoplasias of individuals from Northern Brazil. The proteins were analyzed by two-dimensional electrophoresis and mass spectrometry. For the identification of differentially expressed proteins, we used statistical tests with bootstrapping resampling to control the type I error in the multiple comparison analyses. We identified 111 proteins involved in gastric carcinogenesis. The computational analysis revealed several proteins involved in the energy production processes and reinforced the Warburg effect in gastric cancer. ENO1 and HSPB1 expression were further evaluated. ENO1 was selected due to its role in aerobic glycolysis that may contribute to the Warburg effect. Although we observed two up-regulated spots of ENO1 in the proteomic analysis, the mean expression of ENO1 was reduced in gastric tumors by western blot. However, mean ENO1 expression seems to increase in more invasive tumors. This lack of correlation between proteomic and western blot analyses may be due to the presence of other ENO1 spots that present a slightly reduced expression, but with a high impact in the mean protein expression. In neoplasias, HSPB1 is induced by cellular stress to protect cells against apoptosis. In the present study, HSPB1 presented an elevated protein and mRNA expression in a subset of gastric cancer samples. However, no association was observed between HSPB1 expression and clinicopathological characteristics. Here, we identified several possible biomarkers of gastric cancer in individuals from Northern Brazil. These biomarkers may be useful for the assessment of prognosis and stratification for therapy if validated in larger clinical study sets.Mariana Ferreira LealJanete ChungDanielle Queiroz CalcagnoPaulo Pimentel AssumpçãoSamia DemachkiIsmael Dale Cotrim Guerreiro da SilvaRoger ChammasRommel Rodríguez BurbanoMarília de Arruda Cardoso SmithPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e42255 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Ferreira Leal Janete Chung Danielle Queiroz Calcagno Paulo Pimentel Assumpção Samia Demachki Ismael Dale Cotrim Guerreiro da Silva Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
description	Gastric cancer is the second leading cause of cancer-related death worldwide. The identification of new cancer biomarkers is necessary to reduce the mortality rates through the development of new screening assays and early diagnosis, as well as new target therapies. In this study, we performed a proteomic analysis of noncardia gastric neoplasias of individuals from Northern Brazil. The proteins were analyzed by two-dimensional electrophoresis and mass spectrometry. For the identification of differentially expressed proteins, we used statistical tests with bootstrapping resampling to control the type I error in the multiple comparison analyses. We identified 111 proteins involved in gastric carcinogenesis. The computational analysis revealed several proteins involved in the energy production processes and reinforced the Warburg effect in gastric cancer. ENO1 and HSPB1 expression were further evaluated. ENO1 was selected due to its role in aerobic glycolysis that may contribute to the Warburg effect. Although we observed two up-regulated spots of ENO1 in the proteomic analysis, the mean expression of ENO1 was reduced in gastric tumors by western blot. However, mean ENO1 expression seems to increase in more invasive tumors. This lack of correlation between proteomic and western blot analyses may be due to the presence of other ENO1 spots that present a slightly reduced expression, but with a high impact in the mean protein expression. In neoplasias, HSPB1 is induced by cellular stress to protect cells against apoptosis. In the present study, HSPB1 presented an elevated protein and mRNA expression in a subset of gastric cancer samples. However, no association was observed between HSPB1 expression and clinicopathological characteristics. Here, we identified several possible biomarkers of gastric cancer in individuals from Northern Brazil. These biomarkers may be useful for the assessment of prognosis and stratification for therapy if validated in larger clinical study sets.
format	article
author	Mariana Ferreira Leal Janete Chung Danielle Queiroz Calcagno Paulo Pimentel Assumpção Samia Demachki Ismael Dale Cotrim Guerreiro da Silva Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith
author_facet	Mariana Ferreira Leal Janete Chung Danielle Queiroz Calcagno Paulo Pimentel Assumpção Samia Demachki Ismael Dale Cotrim Guerreiro da Silva Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith
author_sort	Mariana Ferreira Leal
title	Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
title_short	Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
title_full	Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
title_fullStr	Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
title_full_unstemmed	Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.
title_sort	differential proteomic analysis of noncardia gastric cancer from individuals of northern brazil.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/99414cf4f7884f95bff806d2d113c9f6
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_version_	1718423868776906752

Differential proteomic analysis of noncardia gastric cancer from individuals of northern Brazil.

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