The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.

The mucus that coats the gastrointestinal tract of all mammals is a dynamic and sticky gel layer and represents the first protective barrier between the host and the hostile environment. There is, however, a lack of detailed knowledge about the mucus gel organization because of the high water conten...

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Autores principales:	Valérie Gouyer, Frédéric Gottrand, Jean-Luc Desseyn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9941e66095034f029f2f01e67a942d7f
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spelling	oai:doaj.org-article:9941e66095034f029f2f01e67a942d7f2021-11-18T06:55:52ZThe extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.1932-620310.1371/journal.pone.0018761https://doaj.org/article/9941e66095034f029f2f01e67a942d7f2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533274/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The mucus that coats the gastrointestinal tract of all mammals is a dynamic and sticky gel layer and represents the first protective barrier between the host and the hostile environment. There is, however, a lack of detailed knowledge about the mucus gel organization because of the high water content and the complexity of MUC2, the main gel-forming molecule in the intestine. Histological staining and a multilabel immunofluorescence method were used to examine mucus blankets and Muc2 in mouse colon and ileum samples fixed in Carnoy's solution, unveiling an extraordinarily complex but highly structured mucus gel organization. The inner firmly adherent mucus blanket consists of alternating layers. The thicker outer loosely adherent mucus blanket in the colon is made of alternating laminated layers and loose curl-like structures. The layers consist of Muc2 molecules with different fucosylation states and glycoforms remain unmixed in the mucus. Importantly, distinct goblet cell subpopulations throughout the ileum along the crypt-to-villus axis with an alternation of goblet cells secreting fucosylated and non-fucosylated Muc2 are observed. A better understanding of the mucus structure should contribute to improve the efficiency of DNA and drug delivery and will allow for a better understanding and treatment of inflammatory and infectious intestinal diseases.Valérie GouyerFrédéric GottrandJean-Luc DesseynPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18761 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
description	The mucus that coats the gastrointestinal tract of all mammals is a dynamic and sticky gel layer and represents the first protective barrier between the host and the hostile environment. There is, however, a lack of detailed knowledge about the mucus gel organization because of the high water content and the complexity of MUC2, the main gel-forming molecule in the intestine. Histological staining and a multilabel immunofluorescence method were used to examine mucus blankets and Muc2 in mouse colon and ileum samples fixed in Carnoy's solution, unveiling an extraordinarily complex but highly structured mucus gel organization. The inner firmly adherent mucus blanket consists of alternating layers. The thicker outer loosely adherent mucus blanket in the colon is made of alternating laminated layers and loose curl-like structures. The layers consist of Muc2 molecules with different fucosylation states and glycoforms remain unmixed in the mucus. Importantly, distinct goblet cell subpopulations throughout the ileum along the crypt-to-villus axis with an alternation of goblet cells secreting fucosylated and non-fucosylated Muc2 are observed. A better understanding of the mucus structure should contribute to improve the efficiency of DNA and drug delivery and will allow for a better understanding and treatment of inflammatory and infectious intestinal diseases.
format	article
author	Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn
author_facet	Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn
author_sort	Valérie Gouyer
title	The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
title_short	The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
title_full	The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
title_fullStr	The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
title_full_unstemmed	The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
title_sort	extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/9941e66095034f029f2f01e67a942d7f
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The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.

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