Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo

Masamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the prese...

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Autores principales:	Fukuda M, Sasaki H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/9944ce4db6ba440e9c680234c36412a3
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spelling	oai:doaj.org-article:9944ce4db6ba440e9c680234c36412a32021-12-02T04:20:21ZQuantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo1177-54671177-5483https://doaj.org/article/9944ce4db6ba440e9c680234c36412a32012-04-01T00:00:00Zhttp://www.dovepress.com/quantitative-evaluation-of-corneal-epithelial-injury-caused-by-n-hepta-a9707https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Masamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo.Methods: The central area of an albino rabbit&#39;s cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 &micro;L) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed.Results: As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR.Conclusion: The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.Keywords: eyes in vivo, corneal injury, corneal contact lens electrode corneal resistance device, n-heptanolFukuda MSasaki HDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 585-593 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Fukuda M Sasaki H Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
description	Masamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo.Methods: The central area of an albino rabbit&#39;s cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 &micro;L) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed.Results: As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR.Conclusion: The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.Keywords: eyes in vivo, corneal injury, corneal contact lens electrode corneal resistance device, n-heptanol
format	article
author	Fukuda M Sasaki H
author_facet	Fukuda M Sasaki H
author_sort	Fukuda M
title	Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
title_short	Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
title_full	Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
title_fullStr	Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
title_full_unstemmed	Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
title_sort	quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9944ce4db6ba440e9c680234c36412a3
work_keys_str_mv	AT fukudam quantitativeevaluationofcornealepithelialinjurycausedbynheptanolusingacornealresistancemeasuringdeviceinvivo AT sasakih quantitativeevaluationofcornealepithelialinjurycausedbynheptanolusingacornealresistancemeasuringdeviceinvivo
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Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo

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