Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice

Abstract The place conditioning paradigm is an efficient, widely-used method to study mechanisms that underlie appetitive or aversive learning and memory processes. However, pharmacological agents used to induce conditioned place preference (CPP) or aversion (CPA) can per se interfere with learning...

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Autores principales:	Koral Goltseker, Segev Barak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99474aad5c384539a99b7eaf0bfc7174
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spelling	oai:doaj.org-article:99474aad5c384539a99b7eaf0bfc71742021-12-02T11:40:26ZFlood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice10.1038/s41598-018-25568-52045-2322https://doaj.org/article/99474aad5c384539a99b7eaf0bfc71742018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25568-5https://doaj.org/toc/2045-2322Abstract The place conditioning paradigm is an efficient, widely-used method to study mechanisms that underlie appetitive or aversive learning and memory processes. However, pharmacological agents used to induce conditioned place preference (CPP) or aversion (CPA) can per se interfere with learning and memory processing, hence confounding the results. Therefore, non-pharmacological place conditioning procedures are of high importance. Here, we introduce a novel procedure for induction of CPA in mice, by water flooding. We found that pairing a context with immersion in moderately cold shallow water resulted in aversion and avoidance of that context during a place preference test. Importantly, place aversion emerged only when mice experienced the onset of flood during conditioning training, but not when mice were placed in a compartment pre-filled with water. We also found that warm water was not sufficiently aversive to induce CPA. Moreover, CPA was observed after two or three context-flood pairings but not after one or four pairings, suggesting that moderate conditioning intensity produces optimal CPA expression. Thus, flood-induced CPA is a simple, cheap, and efficient procedure to form and measure place aversion memories in mice, using an ethologically-relevant threat.Koral GoltsekerSegev BarakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koral Goltseker Segev Barak Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
description	Abstract The place conditioning paradigm is an efficient, widely-used method to study mechanisms that underlie appetitive or aversive learning and memory processes. However, pharmacological agents used to induce conditioned place preference (CPP) or aversion (CPA) can per se interfere with learning and memory processing, hence confounding the results. Therefore, non-pharmacological place conditioning procedures are of high importance. Here, we introduce a novel procedure for induction of CPA in mice, by water flooding. We found that pairing a context with immersion in moderately cold shallow water resulted in aversion and avoidance of that context during a place preference test. Importantly, place aversion emerged only when mice experienced the onset of flood during conditioning training, but not when mice were placed in a compartment pre-filled with water. We also found that warm water was not sufficiently aversive to induce CPA. Moreover, CPA was observed after two or three context-flood pairings but not after one or four pairings, suggesting that moderate conditioning intensity produces optimal CPA expression. Thus, flood-induced CPA is a simple, cheap, and efficient procedure to form and measure place aversion memories in mice, using an ethologically-relevant threat.
format	article
author	Koral Goltseker Segev Barak
author_facet	Koral Goltseker Segev Barak
author_sort	Koral Goltseker
title	Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
title_short	Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
title_full	Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
title_fullStr	Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
title_full_unstemmed	Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
title_sort	flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/99474aad5c384539a99b7eaf0bfc7174
work_keys_str_mv	AT koralgoltseker floodconditionedplaceaversionasanovelnonpharmacologicalaversivelearningprocedureinmice AT segevbarak floodconditionedplaceaversionasanovelnonpharmacologicalaversivelearningprocedureinmice
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Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice

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