Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice

Wakako Yamada1, Sadatomo Tasaka1, Hidefumi Koh1, Mie Shimizu1, Yuko Ogawa1, Naoki Hasegawa1, Taku Miyasho2, Kazuhiro Yamaguchi1, Akitoshi Ishizaka11Division of Pulmonary Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan; 2Laboratory of Veterinary Biochemistry, School of Veterin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wakako Yamada, Sadatomo Tasaka, Hidefumi Koh, Mie Shimizu, Yuko Ogawa, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9948281033aa44e3af7038d4f2fa782a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9948281033aa44e3af7038d4f2fa782a
record_format	dspace
spelling	oai:doaj.org-article:9948281033aa44e3af7038d4f2fa782a2021-12-02T00:35:55ZRole of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice1178-7031https://doaj.org/article/9948281033aa44e3af7038d4f2fa782a2008-09-01T00:00:00Zhttp://www.dovepress.com/role-of-toll-like-receptor-4-in-acute-neutrophilic-lung-inflammation-i-a2156https://doaj.org/toc/1178-7031Wakako Yamada1, Sadatomo Tasaka1, Hidefumi Koh1, Mie Shimizu1, Yuko Ogawa1, Naoki Hasegawa1, Taku Miyasho2, Kazuhiro Yamaguchi1, Akitoshi Ishizaka11Division of Pulmonary Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan; 2Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanBackground: Toll-like receptors (TLRs) represent a conserved family of innate immune recognition receptors. Among TLRs, TLR4 is important for the recognition of Gram-negative bacteria, whereas TLR2 recognizes cell wall constituents of Gram-positive microorganisms, such as peptidoglycan (PGN).Methods: To evaluate the role of TLR4 in the pathogenesis of acute lung injury induced by Escherichia coli endotoxin (lipopolysaccharide; LPS) or PGN, we compared inflammatory cell accumulation in bronchoalveolar lavage (BAL) fluid and lung pathology between C3H/HeJ (TLR4 mutant) and wild-type C3H/HeN mice. The levels of proinflammatory cytokines and chemokines in plasma and BAL fluid and nuclear factor-&kappa;B (NF-&kappa;B) translocation in the lung were also evaluated.Results: In C3H/HeJ mice, LPS-induced neutrophil emigration was significantly decreased compared with C3H/HeN mice, whereas PGN-induced neutrophil emigration did not differ. Differential cell count in BAL fluid revealed comparable neutrophil recruitment in the alveolar space. In TLR4 mutant mice, LPS-induced upregulation of tumor necrosis factor-alpha (TNF-&alpha;), KC, and CXCL10 in plasma and BAL fluid was attenuate, which was not different after PGN. NF-&kappa;B translocation in the lung was significantly decreased in C3H/HeJ compared with C3H/HeN mice, whereas PGN-induced NF-&kappa;B translocation was not different.Conclusion: These results suggest that TLR4 mediates inflammatory cascade induced by Gram-negative bacteria that is locally administered.Keywords: rodent, TLR4, endotoxin, neutrophils, NF-&kappa;B Wakako YamadaSadatomo TasakaHidefumi KohMie ShimizuYuko Ogawaet alDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2008, Iss default, Pp 1-10 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wakako Yamada Sadatomo Tasaka Hidefumi Koh Mie Shimizu Yuko Ogawa et al Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
description	Wakako Yamada1, Sadatomo Tasaka1, Hidefumi Koh1, Mie Shimizu1, Yuko Ogawa1, Naoki Hasegawa1, Taku Miyasho2, Kazuhiro Yamaguchi1, Akitoshi Ishizaka11Division of Pulmonary Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan; 2Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanBackground: Toll-like receptors (TLRs) represent a conserved family of innate immune recognition receptors. Among TLRs, TLR4 is important for the recognition of Gram-negative bacteria, whereas TLR2 recognizes cell wall constituents of Gram-positive microorganisms, such as peptidoglycan (PGN).Methods: To evaluate the role of TLR4 in the pathogenesis of acute lung injury induced by Escherichia coli endotoxin (lipopolysaccharide; LPS) or PGN, we compared inflammatory cell accumulation in bronchoalveolar lavage (BAL) fluid and lung pathology between C3H/HeJ (TLR4 mutant) and wild-type C3H/HeN mice. The levels of proinflammatory cytokines and chemokines in plasma and BAL fluid and nuclear factor-&kappa;B (NF-&kappa;B) translocation in the lung were also evaluated.Results: In C3H/HeJ mice, LPS-induced neutrophil emigration was significantly decreased compared with C3H/HeN mice, whereas PGN-induced neutrophil emigration did not differ. Differential cell count in BAL fluid revealed comparable neutrophil recruitment in the alveolar space. In TLR4 mutant mice, LPS-induced upregulation of tumor necrosis factor-alpha (TNF-&alpha;), KC, and CXCL10 in plasma and BAL fluid was attenuate, which was not different after PGN. NF-&kappa;B translocation in the lung was significantly decreased in C3H/HeJ compared with C3H/HeN mice, whereas PGN-induced NF-&kappa;B translocation was not different.Conclusion: These results suggest that TLR4 mediates inflammatory cascade induced by Gram-negative bacteria that is locally administered.Keywords: rodent, TLR4, endotoxin, neutrophils, NF-&kappa;B
format	article
author	Wakako Yamada Sadatomo Tasaka Hidefumi Koh Mie Shimizu Yuko Ogawa et al
author_facet	Wakako Yamada Sadatomo Tasaka Hidefumi Koh Mie Shimizu Yuko Ogawa et al
author_sort	Wakako Yamada
title	Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
title_short	Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
title_full	Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
title_fullStr	Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
title_full_unstemmed	Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
title_sort	role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/9948281033aa44e3af7038d4f2fa782a
work_keys_str_mv	AT wakakoyamada roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice AT sadatomotasaka roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice AT hidefumikoh roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice AT mieshimizu roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice AT yukoogawa roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice AT etal roleoftolllikereceptor4inacuteneutrophiliclunginflammationinducedbyintratrachealbacterialproductsinmice
_version_	1718403633850089472

Role of toll-like receptor 4 in acute neutrophilic lung inflammation induced by intratracheal bacterial products in mice

Ejemplares similares