Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice

Abstract Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative tranquilizing effects. However, the component of G. lucidum that promotes sleep has not been clearly identified. Here, the effect and mechanism of the acidic part of the alcohol extract of G. lucidu...

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Autores principales:	Chunyan Yao, Zhiyuan Wang, Huiyong Jiang, Ren Yan, Qianfei Huang, Yin Wang, Hui Xie, Ying Zou, Ying Yu, Longxian Lv
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/994d3828282144df86e7952819e3a0f2
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spelling	oai:doaj.org-article:994d3828282144df86e7952819e3a0f22021-12-02T16:32:02ZGanoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice10.1038/s41598-021-92913-62045-2322https://doaj.org/article/994d3828282144df86e7952819e3a0f22021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92913-6https://doaj.org/toc/2045-2322Abstract Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative tranquilizing effects. However, the component of G. lucidum that promotes sleep has not been clearly identified. Here, the effect and mechanism of the acidic part of the alcohol extract of G. lucidum mycelia (GLAA) on sleep were studied in mice. Administration of 25, 50 and 100 mg/kg GLAA for 28 days promoted sleep in pentobarbital-treated mice by shortening sleep latency and prolonging sleeping time. GLAA administration increased the levels of the sleep-promoting neurotransmitter 5-hydroxytryptamine and the Tph2, Iptr3 and Gng13 transcripts in the sleep-regulating serotonergic synapse pathway in the hypothalamus during this process. Moreover, GLAA administration reduced lipopolysaccharide and raised peptidoglycan levels in serum. GLAA-enriched gut bacteria and metabolites, including Bifidobacterium, Bifidobacterium animalis, indole-3-carboxylic acid and acetylphosphate were negatively correlated with sleep latency and positively correlated with sleeping time and the hypothalamus 5-hydroxytryptamine concentration. Both the GLAA sleep promotion effect and the altered faecal metabolites correlated with sleep behaviours disappeared after gut microbiota depletion with antibiotics. Our results showed that GLAA promotes sleep through a gut microbiota-dependent and serotonin-associated pathway in mice.Chunyan YaoZhiyuan WangHuiyong JiangRen YanQianfei HuangYin WangHui XieYing ZouYing YuLongxian LvNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunyan Yao Zhiyuan Wang Huiyong Jiang Ren Yan Qianfei Huang Yin Wang Hui Xie Ying Zou Ying Yu Longxian Lv Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
description	Abstract Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative tranquilizing effects. However, the component of G. lucidum that promotes sleep has not been clearly identified. Here, the effect and mechanism of the acidic part of the alcohol extract of G. lucidum mycelia (GLAA) on sleep were studied in mice. Administration of 25, 50 and 100 mg/kg GLAA for 28 days promoted sleep in pentobarbital-treated mice by shortening sleep latency and prolonging sleeping time. GLAA administration increased the levels of the sleep-promoting neurotransmitter 5-hydroxytryptamine and the Tph2, Iptr3 and Gng13 transcripts in the sleep-regulating serotonergic synapse pathway in the hypothalamus during this process. Moreover, GLAA administration reduced lipopolysaccharide and raised peptidoglycan levels in serum. GLAA-enriched gut bacteria and metabolites, including Bifidobacterium, Bifidobacterium animalis, indole-3-carboxylic acid and acetylphosphate were negatively correlated with sleep latency and positively correlated with sleeping time and the hypothalamus 5-hydroxytryptamine concentration. Both the GLAA sleep promotion effect and the altered faecal metabolites correlated with sleep behaviours disappeared after gut microbiota depletion with antibiotics. Our results showed that GLAA promotes sleep through a gut microbiota-dependent and serotonin-associated pathway in mice.
format	article
author	Chunyan Yao Zhiyuan Wang Huiyong Jiang Ren Yan Qianfei Huang Yin Wang Hui Xie Ying Zou Ying Yu Longxian Lv
author_facet	Chunyan Yao Zhiyuan Wang Huiyong Jiang Ren Yan Qianfei Huang Yin Wang Hui Xie Ying Zou Ying Yu Longxian Lv
author_sort	Chunyan Yao
title	Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
title_short	Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
title_full	Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
title_fullStr	Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
title_full_unstemmed	Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
title_sort	ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/994d3828282144df86e7952819e3a0f2
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Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice

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