Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer

Hongmei Zheng,1 Xiang Li,1 Chuang Chen,2 Jian Chen,3 Jinzhong Sun,2 Si Sun,4 Liting Jin,1 Juanjuan Li,2 Shengrong Sun,2 Xinhong Wu1 1Department of Breast Surgery, Hubei Cancer Hospital, 2Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, 3Department of Head and Neck Surg...

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Autores principales:	Zheng H, Li X, Chen C, Chen J, Sun J, Sun S, Jin L, Li J, Wu X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	triple negative breast cancer quantum dot TOP2A disease free survival prognosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/99570acb25da4fb081423ecb3afaeb26
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spelling	oai:doaj.org-article:99570acb25da4fb081423ecb3afaeb262021-12-02T02:37:16ZQuantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer1178-2013https://doaj.org/article/99570acb25da4fb081423ecb3afaeb262016-10-01T00:00:00Zhttps://www.dovepress.com/quantum-dot-based-immunofluorescent-imaging-and-quantitative-detection-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hongmei Zheng,1 Xiang Li,1 Chuang Chen,2 Jian Chen,3 Jinzhong Sun,2 Si Sun,4 Liting Jin,1 Juanjuan Li,2 Shengrong Sun,2 Xinhong Wu1 1Department of Breast Surgery, Hubei Cancer Hospital, 2Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, 3Department of Head and Neck Surgery, Hubei Cancer Hospital, 4Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China Background: Topoisomerase 2 alpha (TOP2A) is a key enzyme in DNA replication and a target of various cytotoxic agents including anthracyclines. Previous studies evaluating the predictive and prognostic values of TOP2A in breast cancer are contradictory, likely secondary to the use of both different detection methods and different cutoff thresholds for positive status. Our own studies have previously confirmed the advantages of quantum dot-based nanotechnology for quantitative analysis of biomarkers relative to conventional immunohistochemistry (IHC). This study was designed to 1) assess the expression of TOP2A, 2) investigate the relationship between TOP2A expression and major clinical pathological parameters, and 3) evaluate the prognostic value of TOP2A by quantum dot-based immunofluorescent imaging and quantitative analytical system (QD-IIQAS) in triple-negative breast cancer (TNBC).Patients and methods: TOP2A expression in 145 TNBC specimens was detected using IHC and QD-IIQAS, and a comparative analysis of the two methods was conducted, including an exploration of the relationship between TOP2A expression and major clinical pathological parameters in TNBC. The prognostic value of TOP2A in TNBC was assessed.Results: A similar antigen localization, a high correlation of staining rates (r=0.79), and a high agreement of measurements (κ=0.763) of TOP2A expression in TNBC were found by QD-IIQAS and conventional IHC (cutoff: 45.0 and 0.45, respectively). TOP2A was significantly higher in larger tumors (P=0.002), higher grade tumors (P=0.005), and lymph node positive patients (P<0.001). The 5-year disease-free survival (5-DFS) of the high and low TOP2A subgroups was significantly different for both QD-IIQAS and IHC (P<0.001, log-rank test for both). TOP2A expression was an independent predictor of survival in TNBC (P=0.001).Conclusion: QD-IIQAS was an easy and accurate method for detecting and assessing TOP2A. The TOP2A expression was an independent prognostic indicator of 5-DFS in TNBC. Our study provides a good foundation for future studies exploring the relationship between TOP2A expression and response to anthracyclines. Keywords: triple-negative breast cancer, quantum dot, TOP2A, disease-free survival, prognosisZheng HLi XChen CChen JSun JSun SJin LLi JSun SWu XDove Medical Pressarticletriple negative breast cancerquantum dotTOP2Adisease free survivalprognosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5519-5529 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	triple negative breast cancer quantum dot TOP2A disease free survival prognosis Medicine (General) R5-920
spellingShingle	triple negative breast cancer quantum dot TOP2A disease free survival prognosis Medicine (General) R5-920 Zheng H Li X Chen C Chen J Sun J Sun S Jin L Li J Sun S Wu X Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
description	Hongmei Zheng,1 Xiang Li,1 Chuang Chen,2 Jian Chen,3 Jinzhong Sun,2 Si Sun,4 Liting Jin,1 Juanjuan Li,2 Shengrong Sun,2 Xinhong Wu1 1Department of Breast Surgery, Hubei Cancer Hospital, 2Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, 3Department of Head and Neck Surgery, Hubei Cancer Hospital, 4Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China Background: Topoisomerase 2 alpha (TOP2A) is a key enzyme in DNA replication and a target of various cytotoxic agents including anthracyclines. Previous studies evaluating the predictive and prognostic values of TOP2A in breast cancer are contradictory, likely secondary to the use of both different detection methods and different cutoff thresholds for positive status. Our own studies have previously confirmed the advantages of quantum dot-based nanotechnology for quantitative analysis of biomarkers relative to conventional immunohistochemistry (IHC). This study was designed to 1) assess the expression of TOP2A, 2) investigate the relationship between TOP2A expression and major clinical pathological parameters, and 3) evaluate the prognostic value of TOP2A by quantum dot-based immunofluorescent imaging and quantitative analytical system (QD-IIQAS) in triple-negative breast cancer (TNBC).Patients and methods: TOP2A expression in 145 TNBC specimens was detected using IHC and QD-IIQAS, and a comparative analysis of the two methods was conducted, including an exploration of the relationship between TOP2A expression and major clinical pathological parameters in TNBC. The prognostic value of TOP2A in TNBC was assessed.Results: A similar antigen localization, a high correlation of staining rates (r=0.79), and a high agreement of measurements (κ=0.763) of TOP2A expression in TNBC were found by QD-IIQAS and conventional IHC (cutoff: 45.0 and 0.45, respectively). TOP2A was significantly higher in larger tumors (P=0.002), higher grade tumors (P=0.005), and lymph node positive patients (P<0.001). The 5-year disease-free survival (5-DFS) of the high and low TOP2A subgroups was significantly different for both QD-IIQAS and IHC (P<0.001, log-rank test for both). TOP2A expression was an independent predictor of survival in TNBC (P=0.001).Conclusion: QD-IIQAS was an easy and accurate method for detecting and assessing TOP2A. The TOP2A expression was an independent prognostic indicator of 5-DFS in TNBC. Our study provides a good foundation for future studies exploring the relationship between TOP2A expression and response to anthracyclines. Keywords: triple-negative breast cancer, quantum dot, TOP2A, disease-free survival, prognosis
format	article
author	Zheng H Li X Chen C Chen J Sun J Sun S Jin L Li J Sun S Wu X
author_facet	Zheng H Li X Chen C Chen J Sun J Sun S Jin L Li J Sun S Wu X
author_sort	Zheng H
title	Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
title_short	Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
title_full	Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
title_fullStr	Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
title_full_unstemmed	Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer
title_sort	quantum dot-based immunofluorescent imaging and quantitative detection of top2a and prognostic value in triple-negative breast cancer
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/99570acb25da4fb081423ecb3afaeb26
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Quantum dot-based immunofluorescent imaging and quantitative detection of TOP2A and prognostic value in triple-negative breast cancer

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