An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states

Crowding effects—important when considering cellular environments—greatly influence protein stability. Here the authors study the impact of macromolecular crowders on high and low temperature protein unfolding, and show that volume exclusion effects are larger when the protein and crowder volumes ar...

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Autores principales: Caterina Alfano, Domenico Sanfelice, Stephen R. Martin, Annalisa Pastore, Piero Andrea Temussi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/996b0550a8f049cd81c7f7723f6cdd5f
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