Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape

Sugar content directly affects grape (Vitis vinifera L.) berry quality and the resulting wine. Therefore, it is of great importance to study and explore novel genes that affect sugar accumulation in grapes. Glycosyl hydrolases (GHs) are key enzymes hydrolyzing polysaccharides into...

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Autores principales:	Guangzhao Xu, Yi Wang, Chong Ren, Peige Fan, Yangfu Kuang, Yue Wang, Zhenchang Liang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	grape sugar <i>VvGH9</i> transcriptomic analysis overexpression Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/996b3c5dc5054c469c81adb8a04765d1
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spelling	oai:doaj.org-article:996b3c5dc5054c469c81adb8a04765d12021-11-25T17:47:19ZGenome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape10.3390/horticulturae71104532311-7524https://doaj.org/article/996b3c5dc5054c469c81adb8a04765d12021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/453https://doaj.org/toc/2311-7524Sugar content directly affects grape (<i>Vitis vinifera</i> L.) berry quality and the resulting wine. Therefore, it is of great importance to study and explore novel genes that affect sugar accumulation in grapes. Glycosyl hydrolases (GHs) are key enzymes hydrolyzing polysaccharides into monosaccharides and play important roles in the regulation of carbohydrate metabolism. Nevertheless, the impact of GHs on the regulation of sugar accumulation in plants has rarely been investigated. In this study, we identified 11 putative <i>GH</i> genes in grapevines by phylogeny analysis. RNA-seq and quantitative real-time PCR results demonstrated that the expression level of <i>VvGH9</i> was higher during the fruit set stage, which had lower sugar content than the véraison and ripe stages. Treatment of grape berries with exogenous sugar two weeks before véraison revealed that <i>VvGH9</i> was rapidly induced by sucrose, fructose, and glucose. When ‘41B’ calli was treated with different concentrations of glucose, <i>VvGH9</i> expression increased at first and then decreased with the increase of glucose concentration. Overexpression of <i>VvGH9</i> in grape calli and tomatoes also confirmed that this gene could contribute to sugar accumulation. All the above results demonstrated that <i>VvGH9</i> promotes sugar accumulation under low sugar content in plants.Guangzhao XuYi WangChong RenPeige FanYangfu KuangYue WangZhenchang LiangMDPI AGarticlegrapesugar<i>VvGH9</i>transcriptomic analysisoverexpressionPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 453, p 453 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	grape sugar <i>VvGH9</i> transcriptomic analysis overexpression Plant culture SB1-1110
spellingShingle	grape sugar <i>VvGH9</i> transcriptomic analysis overexpression Plant culture SB1-1110 Guangzhao Xu Yi Wang Chong Ren Peige Fan Yangfu Kuang Yue Wang Zhenchang Liang Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
description	Sugar content directly affects grape (<i>Vitis vinifera</i> L.) berry quality and the resulting wine. Therefore, it is of great importance to study and explore novel genes that affect sugar accumulation in grapes. Glycosyl hydrolases (GHs) are key enzymes hydrolyzing polysaccharides into monosaccharides and play important roles in the regulation of carbohydrate metabolism. Nevertheless, the impact of GHs on the regulation of sugar accumulation in plants has rarely been investigated. In this study, we identified 11 putative <i>GH</i> genes in grapevines by phylogeny analysis. RNA-seq and quantitative real-time PCR results demonstrated that the expression level of <i>VvGH9</i> was higher during the fruit set stage, which had lower sugar content than the véraison and ripe stages. Treatment of grape berries with exogenous sugar two weeks before véraison revealed that <i>VvGH9</i> was rapidly induced by sucrose, fructose, and glucose. When ‘41B’ calli was treated with different concentrations of glucose, <i>VvGH9</i> expression increased at first and then decreased with the increase of glucose concentration. Overexpression of <i>VvGH9</i> in grape calli and tomatoes also confirmed that this gene could contribute to sugar accumulation. All the above results demonstrated that <i>VvGH9</i> promotes sugar accumulation under low sugar content in plants.
format	article
author	Guangzhao Xu Yi Wang Chong Ren Peige Fan Yangfu Kuang Yue Wang Zhenchang Liang
author_facet	Guangzhao Xu Yi Wang Chong Ren Peige Fan Yangfu Kuang Yue Wang Zhenchang Liang
author_sort	Guangzhao Xu
title	Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
title_short	Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
title_full	Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
title_fullStr	Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
title_full_unstemmed	Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
title_sort	genome wide analysis of <i>gh</i> gene family reveals <i>vvgh9</i> positively regulates sugar accumulation under low sugar content in grape
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/996b3c5dc5054c469c81adb8a04765d1
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Genome Wide Analysis of <i>GH</i> Gene Family Reveals <i>Vvgh9</i> Positively Regulates Sugar Accumulation under Low Sugar Content in Grape

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