Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro

The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the format...

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Autores principales:	Takahiro Hosoya, Ikumi Tsuchiya, Toshiro Ohta, Mokhtar Benhanifia, Shigenori Kumazawa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	propolis antiangiogenic ferulenol plant origin Algeria Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/997959202c2b4d1484d34fc8fd900f6b
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spelling	oai:doaj.org-article:997959202c2b4d1484d34fc8fd900f6b2021-11-11T18:30:32ZComposition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro10.3390/molecules262165101420-3049https://doaj.org/article/997959202c2b4d1484d34fc8fd900f6b2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6510https://doaj.org/toc/1420-3049The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the formation of capillary networks in a dose-dependent manner (6.25–50 μg/mL) within 12 h and induced cell fragmentation of HUVECs at 50 μg/mL after treatment for 24 h. To identify the active compounds in EEAP1, a high-performance liquid chromatography (HPLC) analysis was performed, revealing that EEAP1 contains two major compounds. Both compounds were isolated by repeated column chromatography and identified as ω-hydroxyferulenol (<b>1</b>) and ferulenol (<b>2</b>), which have a coumarin structure conjugated with a farnesyl group according to NMR, high-resolution electrospray ionization mass spectroscopy, and chemical modification. Compounds <b>1</b> and <b>2</b> inhibited the tube-forming activity of HUVECs, especially <b>2</b>, which exhibited a stronger antiangiogenic effect even at a low concentration of 3.31 μg/mL. Moreover, <b>2</b> suppressed the elongation and induced cell fragmentation at the same dose. The molecular changes in tube-forming HUVECs induced by <b>2</b> were found to be related to the activation of the caspase signals. To confirm the plant origin of propolis, an HPLC comparative analysis of the ethanol extracts of some plants near beekeeping areas and that of Algerian propolis (EEAP1) was performed, and similar chromatographic patterns were observed. This result suggests that the plant origin of this Algerian propolis is the resin of <i>Ferula communis</i>.Takahiro HosoyaIkumi TsuchiyaToshiro OhtaMokhtar BenhanifiaShigenori KumazawaMDPI AGarticlepropolisantiangiogenicferulenolplant originAlgeriaOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6510, p 6510 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis antiangiogenic ferulenol plant origin Algeria Organic chemistry QD241-441
spellingShingle	propolis antiangiogenic ferulenol plant origin Algeria Organic chemistry QD241-441 Takahiro Hosoya Ikumi Tsuchiya Toshiro Ohta Mokhtar Benhanifia Shigenori Kumazawa Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
description	The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the formation of capillary networks in a dose-dependent manner (6.25–50 μg/mL) within 12 h and induced cell fragmentation of HUVECs at 50 μg/mL after treatment for 24 h. To identify the active compounds in EEAP1, a high-performance liquid chromatography (HPLC) analysis was performed, revealing that EEAP1 contains two major compounds. Both compounds were isolated by repeated column chromatography and identified as ω-hydroxyferulenol (<b>1</b>) and ferulenol (<b>2</b>), which have a coumarin structure conjugated with a farnesyl group according to NMR, high-resolution electrospray ionization mass spectroscopy, and chemical modification. Compounds <b>1</b> and <b>2</b> inhibited the tube-forming activity of HUVECs, especially <b>2</b>, which exhibited a stronger antiangiogenic effect even at a low concentration of 3.31 μg/mL. Moreover, <b>2</b> suppressed the elongation and induced cell fragmentation at the same dose. The molecular changes in tube-forming HUVECs induced by <b>2</b> were found to be related to the activation of the caspase signals. To confirm the plant origin of propolis, an HPLC comparative analysis of the ethanol extracts of some plants near beekeeping areas and that of Algerian propolis (EEAP1) was performed, and similar chromatographic patterns were observed. This result suggests that the plant origin of this Algerian propolis is the resin of <i>Ferula communis</i>.
format	article
author	Takahiro Hosoya Ikumi Tsuchiya Toshiro Ohta Mokhtar Benhanifia Shigenori Kumazawa
author_facet	Takahiro Hosoya Ikumi Tsuchiya Toshiro Ohta Mokhtar Benhanifia Shigenori Kumazawa
author_sort	Takahiro Hosoya
title	Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_short	Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_full	Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_fullStr	Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_full_unstemmed	Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_sort	composition of algerian propolis, plant origin, and its antiangiogenic activity in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/997959202c2b4d1484d34fc8fd900f6b
work_keys_str_mv	AT takahirohosoya compositionofalgerianpropolisplantoriginanditsantiangiogenicactivityinvitro AT ikumitsuchiya compositionofalgerianpropolisplantoriginanditsantiangiogenicactivityinvitro AT toshiroohta compositionofalgerianpropolisplantoriginanditsantiangiogenicactivityinvitro AT mokhtarbenhanifia compositionofalgerianpropolisplantoriginanditsantiangiogenicactivityinvitro AT shigenorikumazawa compositionofalgerianpropolisplantoriginanditsantiangiogenicactivityinvitro
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Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro

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